Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in Dogs
The histopathological effects of three levels of ischemia-reperfusion (IR) on healing of intestinal anastomotic wound were investigated in dogs. Three groups of dogs in which superior mesenteric artery (SMA) and collaterals were isolated but not occluded (A -control), isolated and occluded immediate...
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Sociedad Chilena de Anatomía
2007
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oai:scielo:S0717-950220070004000202008-04-15Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in DogsAkinrinmade,J. FOlatunji-Akioye,A. O Histopathology Ischemia-reperfusion injury Intestinal anastomoses Wound healing The histopathological effects of three levels of ischemia-reperfusion (IR) on healing of intestinal anastomotic wound were investigated in dogs. Three groups of dogs in which superior mesenteric artery (SMA) and collaterals were isolated but not occluded (A -control), isolated and occluded immediately distal to the aorta with collateral interruption using an atraumatic arterial clip for fifteen minutes (B) and for forty-five minutes (C) prior to ileal resection and anastomosis after return of normal perfusión in occluded groups was done. Within each group, animals were anaesthesized on third and seventh post-operative day. Grossly, impaired abdominal wound healing, intra-abdominal adhesions and partial and complete anastomotic dehiscence occurred in groups B and C commensurate with intensity of ischemic injury. Histologically, light cellular infiltration with loose granulation tissue was observed in the control group with moderate neovascularization and epithehalization by the seventh day. With mild IR, moderate cellular infiltration was observed four days post operation, but heamorrhage and oedema persisted with minimal mucosal regeneration at seven days post operation. With profound IR, sloughing of the mucosa with cellular infiltration at four days with absence of mucosal regeneration and persistence of inflammatory cellular infiltrates minimal neovascularization and dense granulation tissue. Neutrophilia in the immediate post operative period appears significant to impaired healing due to ischemia-reperfusioninfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.25 n.4 20072007-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400020en10.4067/S0717-95022007000400020 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Histopathology Ischemia-reperfusion injury Intestinal anastomoses Wound healing |
| spellingShingle |
Histopathology Ischemia-reperfusion injury Intestinal anastomoses Wound healing Akinrinmade,J. F Olatunji-Akioye,A. O Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in Dogs |
| description |
The histopathological effects of three levels of ischemia-reperfusion (IR) on healing of intestinal anastomotic wound were investigated in dogs. Three groups of dogs in which superior mesenteric artery (SMA) and collaterals were isolated but not occluded (A -control), isolated and occluded immediately distal to the aorta with collateral interruption using an atraumatic arterial clip for fifteen minutes (B) and for forty-five minutes (C) prior to ileal resection and anastomosis after return of normal perfusión in occluded groups was done. Within each group, animals were anaesthesized on third and seventh post-operative day. Grossly, impaired abdominal wound healing, intra-abdominal adhesions and partial and complete anastomotic dehiscence occurred in groups B and C commensurate with intensity of ischemic injury. Histologically, light cellular infiltration with loose granulation tissue was observed in the control group with moderate neovascularization and epithehalization by the seventh day. With mild IR, moderate cellular infiltration was observed four days post operation, but heamorrhage and oedema persisted with minimal mucosal regeneration at seven days post operation. With profound IR, sloughing of the mucosa with cellular infiltration at four days with absence of mucosal regeneration and persistence of inflammatory cellular infiltrates minimal neovascularization and dense granulation tissue. Neutrophilia in the immediate post operative period appears significant to impaired healing due to ischemia-reperfusion |
| author |
Akinrinmade,J. F Olatunji-Akioye,A. O |
| author_facet |
Akinrinmade,J. F Olatunji-Akioye,A. O |
| author_sort |
Akinrinmade,J. F |
| title |
Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in Dogs |
| title_short |
Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in Dogs |
| title_full |
Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in Dogs |
| title_fullStr |
Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in Dogs |
| title_full_unstemmed |
Histopathological Effects of Ischemia - Reperfusion on Intestinal Anastomotic Wound Healing in Dogs |
| title_sort |
histopathological effects of ischemia - reperfusion on intestinal anastomotic wound healing in dogs |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2007 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400020 |
| work_keys_str_mv |
AT akinrinmadejf histopathologicaleffectsofischemiareperfusiononintestinalanastomoticwoundhealingindogs AT olatunjiakioyeao histopathologicaleffectsofischemiareperfusiononintestinalanastomoticwoundhealingindogs |
| _version_ |
1718444613830705152 |