A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histologic Section of the Testis

This study describes the preparation and use of Roselle (Hibiscus sábddariffa) for the differential staining of testicular tissue sections to find out its staining effect on nuclear, cytoplasmic and other structures. Various treatments using modifications of the plant extract in water were carried o...

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Autores principales:	Egbujo,E. C., Adisa,O. J, Yahaya,A. B
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2008
Materias:	Hibiscus sabdariffa Staining Testis Histologic section
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400022
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spelling	oai:scielo:S0717-950220080004000222009-05-25A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histologic Section of the TestisEgbujo,E. C.Adisa,O. JYahaya,A. B Hibiscus sabdariffa Staining Testis Histologic section This study describes the preparation and use of Roselle (Hibiscus sábddariffa) for the differential staining of testicular tissue sections to find out its staining effect on nuclear, cytoplasmic and other structures. Various treatments using modifications of the plant extract in water were carried out on sections of the rabbit testis. Various levéis of differentiation of nuclear and cytoplasmic structures as well as other structures of this organ was obtained especially when 1% eosin was applied as a counter stain. The best staining result was obtained when iron alum was used to mordant the extract and when the extract, mordanted with potassium alum was acidified using acetic acid and used to stain the sections. Modification of the aqueous extract to an alkaline pH using ammonia gave the poorest staining effect. Roselle extract therefore shows reasonable potential as a candidate nuclear stain especially when modanted with iron alum or mordanted with potassium alum and acidified with acetic acid.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.26 n.4 20082008-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400022en10.4067/S0717-95022008000400022
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Hibiscus sabdariffa Staining Testis Histologic section
spellingShingle	Hibiscus sabdariffa Staining Testis Histologic section Egbujo,E. C. Adisa,O. J Yahaya,A. B A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histologic Section of the Testis
description	This study describes the preparation and use of Roselle (Hibiscus sábddariffa) for the differential staining of testicular tissue sections to find out its staining effect on nuclear, cytoplasmic and other structures. Various treatments using modifications of the plant extract in water were carried out on sections of the rabbit testis. Various levéis of differentiation of nuclear and cytoplasmic structures as well as other structures of this organ was obtained especially when 1% eosin was applied as a counter stain. The best staining result was obtained when iron alum was used to mordant the extract and when the extract, mordanted with potassium alum was acidified using acetic acid and used to stain the sections. Modification of the aqueous extract to an alkaline pH using ammonia gave the poorest staining effect. Roselle extract therefore shows reasonable potential as a candidate nuclear stain especially when modanted with iron alum or mordanted with potassium alum and acidified with acetic acid.
author	Egbujo,E. C. Adisa,O. J Yahaya,A. B
author_facet	Egbujo,E. C. Adisa,O. J Yahaya,A. B
author_sort	Egbujo,E. C.
title	A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histologic Section of the Testis
title_short	A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histologic Section of the Testis
title_full	A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histologic Section of the Testis
title_fullStr	A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histologic Section of the Testis
title_full_unstemmed	A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histologic Section of the Testis
title_sort	study of the staining effect of roselle (hibiscus sabdariffa) on the histologic section of the testis
publisher	Sociedad Chilena de Anatomía
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400022
work_keys_str_mv	AT egbujoec astudyofthestainingeffectofrosellehibiscussabdariffaonthehistologicsectionofthetestis AT adisaoj astudyofthestainingeffectofrosellehibiscussabdariffaonthehistologicsectionofthetestis AT yahayaab astudyofthestainingeffectofrosellehibiscussabdariffaonthehistologicsectionofthetestis AT egbujoec studyofthestainingeffectofrosellehibiscussabdariffaonthehistologicsectionofthetestis AT adisaoj studyofthestainingeffectofrosellehibiscussabdariffaonthehistologicsectionofthetestis AT yahayaab studyofthestainingeffectofrosellehibiscussabdariffaonthehistologicsectionofthetestis
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A Study of the Staining Effect of Roselle (Hibiscus sabdariffa) on the Histologic Section of the Testis

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