Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry

This study aims to evalúate the egg-granuloma system in hepatic tissues using lectin histochemistry in experimental Schistosomiasis. Eight Swiss mice were infected with a local strain of Schistosoma mansoni, being submitted forty days later to a perfusión after which slices of liver (4|J.m) were pre...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ribeiro Melo-Júnior,Mario, Bezerra Cavalcanti,Carmelita de Lima, Teles de Pontes-Filho,Nicodemos, Carvalho Jr,Luiz Bezerra de, Carneiro Beltrão,Eduardo Isidoro
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2008
Materias:	Lectins Egg-granuloma system Liver Schistosoma mansoni
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400030
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022008000400030
record_format	dspace
spelling	oai:scielo:S0717-950220080004000302009-05-25Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin HistochemistryRibeiro Melo-Júnior,MarioBezerra Cavalcanti,Carmelita de LimaTeles de Pontes-Filho,NicodemosCarvalho Jr,Luiz Bezerra deCarneiro Beltrão,Eduardo Isidoro Lectins Egg-granuloma system Liver Schistosoma mansoni This study aims to evalúate the egg-granuloma system in hepatic tissues using lectin histochemistry in experimental Schistosomiasis. Eight Swiss mice were infected with a local strain of Schistosoma mansoni, being submitted forty days later to a perfusión after which slices of liver (4\|J.m) were prepared. The tissue samples were incubated with the following peroxidase conjugated lectins: Peanut agglutinin (PNA), Wheat Germ agglutinin (WGA), and Concanavalin A (Con A). All lectins recognized the glycoconjugates in the adult worm tegument. In the hepatic tissue, WGA presented the highest staining followed by PNA and Con A. The PNA presented the most intense staining of the egg-granuloma system while WGA stained the hepatic sinusoid cells and Con A bound preferentially the fibrosis rings of granuloma and the surrounding hepatic parenquima. WGA and PNA indicated the presence of residues of N-acetyl-glucosamine and galactose in the surface of Schistosoma mansoni eggs in the hepatic granulomas. In conclusión, using PNA, Con A and WGA our study presented different aspects of the egg-granuloma and Tegument of Schistosoma mansoni as well as indicated differences in the peri-ovular granulomas indicating alterations in the cellular mechanism of expression of surface carbohydrates during progression of the Schistosomiasis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.26 n.4 20082008-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400030en10.4067/S0717-95022008000400030
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Lectins Egg-granuloma system Liver Schistosoma mansoni
spellingShingle	Lectins Egg-granuloma system Liver Schistosoma mansoni Ribeiro Melo-Júnior,Mario Bezerra Cavalcanti,Carmelita de Lima Teles de Pontes-Filho,Nicodemos Carvalho Jr,Luiz Bezerra de Carneiro Beltrão,Eduardo Isidoro Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry
description	This study aims to evalúate the egg-granuloma system in hepatic tissues using lectin histochemistry in experimental Schistosomiasis. Eight Swiss mice were infected with a local strain of Schistosoma mansoni, being submitted forty days later to a perfusión after which slices of liver (4\|J.m) were prepared. The tissue samples were incubated with the following peroxidase conjugated lectins: Peanut agglutinin (PNA), Wheat Germ agglutinin (WGA), and Concanavalin A (Con A). All lectins recognized the glycoconjugates in the adult worm tegument. In the hepatic tissue, WGA presented the highest staining followed by PNA and Con A. The PNA presented the most intense staining of the egg-granuloma system while WGA stained the hepatic sinusoid cells and Con A bound preferentially the fibrosis rings of granuloma and the surrounding hepatic parenquima. WGA and PNA indicated the presence of residues of N-acetyl-glucosamine and galactose in the surface of Schistosoma mansoni eggs in the hepatic granulomas. In conclusión, using PNA, Con A and WGA our study presented different aspects of the egg-granuloma and Tegument of Schistosoma mansoni as well as indicated differences in the peri-ovular granulomas indicating alterations in the cellular mechanism of expression of surface carbohydrates during progression of the Schistosomiasis.
author	Ribeiro Melo-Júnior,Mario Bezerra Cavalcanti,Carmelita de Lima Teles de Pontes-Filho,Nicodemos Carvalho Jr,Luiz Bezerra de Carneiro Beltrão,Eduardo Isidoro
author_facet	Ribeiro Melo-Júnior,Mario Bezerra Cavalcanti,Carmelita de Lima Teles de Pontes-Filho,Nicodemos Carvalho Jr,Luiz Bezerra de Carneiro Beltrão,Eduardo Isidoro
author_sort	Ribeiro Melo-Júnior,Mario
title	Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry
title_short	Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry
title_full	Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry
title_fullStr	Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry
title_full_unstemmed	Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry
title_sort	carbohydrates detection in the hepatic egg - granuloma system using lectin histochemistry
publisher	Sociedad Chilena de Anatomía
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400030
work_keys_str_mv	AT ribeiromelojuniormario carbohydratesdetectioninthehepaticegggranulomasystemusinglectinhistochemistry AT bezerracavalcanticarmelitadelima carbohydratesdetectioninthehepaticegggranulomasystemusinglectinhistochemistry AT telesdepontesfilhonicodemos carbohydratesdetectioninthehepaticegggranulomasystemusinglectinhistochemistry AT carvalhojrluizbezerrade carbohydratesdetectioninthehepaticegggranulomasystemusinglectinhistochemistry AT carneirobeltraoeduardoisidoro carbohydratesdetectioninthehepaticegggranulomasystemusinglectinhistochemistry
_version_	1718444641285570560

Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry

Ejemplares similares