Effects of Oral Nicotine on Rat Liver Stereology

Nicotine is the more abundant component in cigarette smoke. Because nicotine is first metabolized in the liver, our aim was to investígate the effects of nicotine on this organ by biochemical and stereological methods. Male Wistar rats were treated with oral nicotine (ON) diluted in drinking water d...

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Autores principales:	Valenca,Samuel Santos, Gouveia,Lucas, Alves Pimenta,Wagner, Porto,Luís Cristóvão
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2008
Materias:	Nicotine Rat Liver Stereology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400037
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spelling	oai:scielo:S0717-950220080004000372009-05-25Effects of Oral Nicotine on Rat Liver StereologyValenca,Samuel SantosGouveia,LucasAlves Pimenta,WagnerPorto,Luís Cristóvão Nicotine Rat Liver Stereology Nicotine is the more abundant component in cigarette smoke. Because nicotine is first metabolized in the liver, our aim was to investígate the effects of nicotine on this organ by biochemical and stereological methods. Male Wistar rats were treated with oral nicotine (ON) diluted in drinking water during 32 days. The control group was treated with drinking water in the same period. Rats were sacrificed 24 hours after last day, the blood was collected and the liver was removed. Lipidogram was performed by enzymatic method and collagen fibers, fat globules and hepatocytes were count in the liver by stereological methods. We observed in control group preserved hepatocytes, with no presence of inflammatory cells. However in the ON group was possible to observe varied size of hepatocytes with cloudy cellular limits and histoarchitecture loss. Capillaries were fully of red blood cells. We observed also an increase of fat globules with small size. We observed in leucogram a reduction of leukocytes number (lymphocytes, neutrophils and monocytes) in the ON group in comparison to control group. The lipidogram showed an increase of triglycerides and total cholesterol for ON group when compared to control group. Moreover, a reduction of HDL- cholesterol was observed in ON group when compared to control group. Numerical density of hepatocytes was lesser in ON group when compared to control group. We suggest harmful effects of oral nicotine in liver induced by toxic mechanism with reduction of hepatocytes number and disturbance in lipid metabolism.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.26 n.4 20082008-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400037en10.4067/S0717-95022008000400037
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Nicotine Rat Liver Stereology
spellingShingle	Nicotine Rat Liver Stereology Valenca,Samuel Santos Gouveia,Lucas Alves Pimenta,Wagner Porto,Luís Cristóvão Effects of Oral Nicotine on Rat Liver Stereology
description	Nicotine is the more abundant component in cigarette smoke. Because nicotine is first metabolized in the liver, our aim was to investígate the effects of nicotine on this organ by biochemical and stereological methods. Male Wistar rats were treated with oral nicotine (ON) diluted in drinking water during 32 days. The control group was treated with drinking water in the same period. Rats were sacrificed 24 hours after last day, the blood was collected and the liver was removed. Lipidogram was performed by enzymatic method and collagen fibers, fat globules and hepatocytes were count in the liver by stereological methods. We observed in control group preserved hepatocytes, with no presence of inflammatory cells. However in the ON group was possible to observe varied size of hepatocytes with cloudy cellular limits and histoarchitecture loss. Capillaries were fully of red blood cells. We observed also an increase of fat globules with small size. We observed in leucogram a reduction of leukocytes number (lymphocytes, neutrophils and monocytes) in the ON group in comparison to control group. The lipidogram showed an increase of triglycerides and total cholesterol for ON group when compared to control group. Moreover, a reduction of HDL- cholesterol was observed in ON group when compared to control group. Numerical density of hepatocytes was lesser in ON group when compared to control group. We suggest harmful effects of oral nicotine in liver induced by toxic mechanism with reduction of hepatocytes number and disturbance in lipid metabolism.
author	Valenca,Samuel Santos Gouveia,Lucas Alves Pimenta,Wagner Porto,Luís Cristóvão
author_facet	Valenca,Samuel Santos Gouveia,Lucas Alves Pimenta,Wagner Porto,Luís Cristóvão
author_sort	Valenca,Samuel Santos
title	Effects of Oral Nicotine on Rat Liver Stereology
title_short	Effects of Oral Nicotine on Rat Liver Stereology
title_full	Effects of Oral Nicotine on Rat Liver Stereology
title_fullStr	Effects of Oral Nicotine on Rat Liver Stereology
title_full_unstemmed	Effects of Oral Nicotine on Rat Liver Stereology
title_sort	effects of oral nicotine on rat liver stereology
publisher	Sociedad Chilena de Anatomía
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400037
work_keys_str_mv	AT valencasamuelsantos effectsoforalnicotineonratliverstereology AT gouveialucas effectsoforalnicotineonratliverstereology AT alvespimentawagner effectsoforalnicotineonratliverstereology AT portoluiscristovao effectsoforalnicotineonratliverstereology
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Effects of Oral Nicotine on Rat Liver Stereology

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