Mixture of Kerosene and Xylene: a Contribution to Clearing Agents

The aim of this study was to substitute costly and hazardous compound- xylene, used as clearing agent, with less costly compounds (mixture of xylene and kerosene) having less toxicity and without compromising the cellular integrity and staining characteristics of the sections. Tissues (liver and kid...

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Autores principales:	Ofusori,David A, Ayoka,Abiodun O, Adeeyo,Olusola A, Adewole,Stephen O
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2009
Materias:	Xylene Kerosene Clearing agent Liver Kidney
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100036
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spelling	oai:scielo:S0717-950220090001000362009-06-24Mixture of Kerosene and Xylene: a Contribution to Clearing AgentsOfusori,David AAyoka,Abiodun OAdeeyo,Olusola AAdewole,Stephen O Xylene Kerosene Clearing agent Liver Kidney The aim of this study was to substitute costly and hazardous compound- xylene, used as clearing agent, with less costly compounds (mixture of xylene and kerosene) having less toxicity and without compromising the cellular integrity and staining characteristics of the sections. Tissues (liver and kidney) obtained from a presumable healthy adult Wistar rat, were fixed in 10% formol saline, separated in to five groups (A, B, C, D and E) and processed for light microscopic study adopting H & E staining procedure. During the clearing section, groups A, B, C, D and E were respectively cleared in solvent 1 (xylene only), solvent 2 (70ml xylene : 30ml kerosene), solvent 3 (50ml xylene : 50ml kerosene), solvent 4 (30ml xylene : 70ml kerosene) and solvent 5 (kerosene only). Our result revealed that tissues in groups A, B and C were properly cleared without any morphological impairment. The staining characteristics were also observed to be very bright. Groups D and E however presented poor staining intensity with reduced cellular details. Semi-stained transparent patches were also noticed. It is inferred from the present investigation that a mixture of xylene and kerosene could be employed in the clearing of tissues only at the prescribed ratio i.e. solvent 2 and solvent 3 without posing any health risk or compromising the cellular integrity.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.27 n.1 20092009-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100036en10.4067/S0717-95022009000100036
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Xylene Kerosene Clearing agent Liver Kidney
spellingShingle	Xylene Kerosene Clearing agent Liver Kidney Ofusori,David A Ayoka,Abiodun O Adeeyo,Olusola A Adewole,Stephen O Mixture of Kerosene and Xylene: a Contribution to Clearing Agents
description	The aim of this study was to substitute costly and hazardous compound- xylene, used as clearing agent, with less costly compounds (mixture of xylene and kerosene) having less toxicity and without compromising the cellular integrity and staining characteristics of the sections. Tissues (liver and kidney) obtained from a presumable healthy adult Wistar rat, were fixed in 10% formol saline, separated in to five groups (A, B, C, D and E) and processed for light microscopic study adopting H & E staining procedure. During the clearing section, groups A, B, C, D and E were respectively cleared in solvent 1 (xylene only), solvent 2 (70ml xylene : 30ml kerosene), solvent 3 (50ml xylene : 50ml kerosene), solvent 4 (30ml xylene : 70ml kerosene) and solvent 5 (kerosene only). Our result revealed that tissues in groups A, B and C were properly cleared without any morphological impairment. The staining characteristics were also observed to be very bright. Groups D and E however presented poor staining intensity with reduced cellular details. Semi-stained transparent patches were also noticed. It is inferred from the present investigation that a mixture of xylene and kerosene could be employed in the clearing of tissues only at the prescribed ratio i.e. solvent 2 and solvent 3 without posing any health risk or compromising the cellular integrity.
author	Ofusori,David A Ayoka,Abiodun O Adeeyo,Olusola A Adewole,Stephen O
author_facet	Ofusori,David A Ayoka,Abiodun O Adeeyo,Olusola A Adewole,Stephen O
author_sort	Ofusori,David A
title	Mixture of Kerosene and Xylene: a Contribution to Clearing Agents
title_short	Mixture of Kerosene and Xylene: a Contribution to Clearing Agents
title_full	Mixture of Kerosene and Xylene: a Contribution to Clearing Agents
title_fullStr	Mixture of Kerosene and Xylene: a Contribution to Clearing Agents
title_full_unstemmed	Mixture of Kerosene and Xylene: a Contribution to Clearing Agents
title_sort	mixture of kerosene and xylene: a contribution to clearing agents
publisher	Sociedad Chilena de Anatomía
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100036
work_keys_str_mv	AT ofusoridavida mixtureofkeroseneandxyleneacontributiontoclearingagents AT ayokaabioduno mixtureofkeroseneandxyleneacontributiontoclearingagents AT adeeyoolusolaa mixtureofkeroseneandxyleneacontributiontoclearingagents AT adewolestepheno mixtureofkeroseneandxyleneacontributiontoclearingagents
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Mixture of Kerosene and Xylene: a Contribution to Clearing Agents

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