Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, Brazil

The myxomatosis is a contagious worldwide disease caused by poxvirus which infects domestic and wild rabbits. In the present study we present two distinct outbreaks of myxomatosis when raising rabbits, one for commercial purpose of production of meat and skins and, another one for the commercializat...

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Autores principales:	Catroxo,M. H. B, Bersano,J. G, Martins,A. M. C. P. F, Petrella,S, Portugal,M. A. S. C, Souza,O. S
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2009
Materias:	Myxomatosis Poxvirus Rabbits Transmission electron microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200039
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spelling	oai:scielo:S0717-950220090002000392009-09-10Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, BrazilCatroxo,M. H. BBersano,J. GMartins,A. M. C. P. FPetrella,SPortugal,M. A. S. CSouza,O. S Myxomatosis Poxvirus Rabbits Transmission electron microscopy The myxomatosis is a contagious worldwide disease caused by poxvirus which infects domestic and wild rabbits. In the present study we present two distinct outbreaks of myxomatosis when raising rabbits, one for commercial purpose of production of meat and skins and, another one for the commercialization of ornamental rabbits. The observed signs were ocular, auricular, nasal, testis lesions and many times scattered throughout the body of the animals. The lesions were characterized by formation of nodules that by palpation disclosed gummy or gelatinous aspect. At the transmission electron microscopy, all the skin and crust samples were analyzed by negative staining technique. A great number of particles with morphology similar to the poxvirus, some enveloped in a brick-shaped and irregular disposition of tubules on the external membrane, measuring 300x240 nm on the average were visualized. Ultrathin sections revealed the presence of intracytoplasmic inclusion bodies surrounded by membrane containing oval particles, measuring 270 x 130 nm, containing nucleus or an internal biconcave (dumbbell-shaped) core. Immature particles (empty), surrounded by membrane were also observed. In addition, intracytoplasmic electron dense inclusion bodies containing viral particles budding of dense amorphous material and intranuclear fibrillar or "digital" inclusions showing a regular striation and arranged in groups were found in the middle of granular material. The nuclei were deformed with densely condensed chromatin forming amorphous and electron dense inclusion bodies. In the immunocytochemistry technique, the antigen-antibody reaction was strongly marked by the particles of colloidal gold, emphasizing the viral particles. The techniques used in this study were important in the diagnosis of the affected animals.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.27 n.2 20092009-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200039en10.4067/S0717-95022009000200039
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Myxomatosis Poxvirus Rabbits Transmission electron microscopy
spellingShingle	Myxomatosis Poxvirus Rabbits Transmission electron microscopy Catroxo,M. H. B Bersano,J. G Martins,A. M. C. P. F Petrella,S Portugal,M. A. S. C Souza,O. S Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, Brazil
description	The myxomatosis is a contagious worldwide disease caused by poxvirus which infects domestic and wild rabbits. In the present study we present two distinct outbreaks of myxomatosis when raising rabbits, one for commercial purpose of production of meat and skins and, another one for the commercialization of ornamental rabbits. The observed signs were ocular, auricular, nasal, testis lesions and many times scattered throughout the body of the animals. The lesions were characterized by formation of nodules that by palpation disclosed gummy or gelatinous aspect. At the transmission electron microscopy, all the skin and crust samples were analyzed by negative staining technique. A great number of particles with morphology similar to the poxvirus, some enveloped in a brick-shaped and irregular disposition of tubules on the external membrane, measuring 300x240 nm on the average were visualized. Ultrathin sections revealed the presence of intracytoplasmic inclusion bodies surrounded by membrane containing oval particles, measuring 270 x 130 nm, containing nucleus or an internal biconcave (dumbbell-shaped) core. Immature particles (empty), surrounded by membrane were also observed. In addition, intracytoplasmic electron dense inclusion bodies containing viral particles budding of dense amorphous material and intranuclear fibrillar or "digital" inclusions showing a regular striation and arranged in groups were found in the middle of granular material. The nuclei were deformed with densely condensed chromatin forming amorphous and electron dense inclusion bodies. In the immunocytochemistry technique, the antigen-antibody reaction was strongly marked by the particles of colloidal gold, emphasizing the viral particles. The techniques used in this study were important in the diagnosis of the affected animals.
author	Catroxo,M. H. B Bersano,J. G Martins,A. M. C. P. F Petrella,S Portugal,M. A. S. C Souza,O. S
author_facet	Catroxo,M. H. B Bersano,J. G Martins,A. M. C. P. F Petrella,S Portugal,M. A. S. C Souza,O. S
author_sort	Catroxo,M. H. B
title	Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, Brazil
title_short	Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, Brazil
title_full	Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, Brazil
title_fullStr	Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, Brazil
title_full_unstemmed	Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, Brazil
title_sort	ultrastructural study of poxvirus causing myxomatosis in rabbits, in são paulo and santa catarina, brazil
publisher	Sociedad Chilena de Anatomía
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200039
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Ultrastructural Study of Poxvirus Causing Myxomatosis in Rabbits, in São Paulo and Santa Catarina, Brazil

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