Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents

Transparency technique to demonstrate cartilage and skeleton has been used from years 70. This consists basically of the muscle digestion through an enzyme (trypsine), and the staining of cartilage and skeleton using alcian blue and alizarin red on specimens previously fixed to formalin. This techni...

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Autores principales:	Cortés-Delgado,Natalia, Pérez-Torres,Jairo, Hoyos,Julio Mario
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2009
Materias:	Chiroptera Rodentia Cartilage Bone Morphology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400032
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spelling	oai:scielo:S0717-950220090004000322010-05-18Staining Procedure of Cartilage and Skeleton in Adult Bats and RodentsCortés-Delgado,NataliaPérez-Torres,JairoHoyos,Julio Mario Chiroptera Rodentia Cartilage Bone Morphology Transparency technique to demonstrate cartilage and skeleton has been used from years 70. This consists basically of the muscle digestion through an enzyme (trypsine), and the staining of cartilage and skeleton using alcian blue and alizarin red on specimens previously fixed to formalin. This technique can be used to study the state of the cartilage and the bones in different stages of the development and to observe abnormalitys. Also it can be used to demonstrate museum specimen skeleton preserved in liquid. In this article is showed the application of this technique for the first time in rodents and adult bats, besides some annotations to optimize the procedure.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.27 n.4 20092009-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400032en10.4067/S0717-95022009000400032
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Chiroptera Rodentia Cartilage Bone Morphology
spellingShingle	Chiroptera Rodentia Cartilage Bone Morphology Cortés-Delgado,Natalia Pérez-Torres,Jairo Hoyos,Julio Mario Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents
description	Transparency technique to demonstrate cartilage and skeleton has been used from years 70. This consists basically of the muscle digestion through an enzyme (trypsine), and the staining of cartilage and skeleton using alcian blue and alizarin red on specimens previously fixed to formalin. This technique can be used to study the state of the cartilage and the bones in different stages of the development and to observe abnormalitys. Also it can be used to demonstrate museum specimen skeleton preserved in liquid. In this article is showed the application of this technique for the first time in rodents and adult bats, besides some annotations to optimize the procedure.
author	Cortés-Delgado,Natalia Pérez-Torres,Jairo Hoyos,Julio Mario
author_facet	Cortés-Delgado,Natalia Pérez-Torres,Jairo Hoyos,Julio Mario
author_sort	Cortés-Delgado,Natalia
title	Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents
title_short	Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents
title_full	Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents
title_fullStr	Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents
title_full_unstemmed	Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents
title_sort	staining procedure of cartilage and skeleton in adult bats and rodents
publisher	Sociedad Chilena de Anatomía
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400032
work_keys_str_mv	AT cortesdelgadonatalia stainingprocedureofcartilageandskeletoninadultbatsandrodents AT pereztorresjairo stainingprocedureofcartilageandskeletoninadultbatsandrodents AT hoyosjuliomario stainingprocedureofcartilageandskeletoninadultbatsandrodents
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Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents

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