Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?

Formaldehyde is used traditionally for fixing the cadaver, and vaporized during dissection and practical studying on cadaver. This study was designed to determine the histopathologic and morphometric changes of rat kidney while all of the experiments were exposed to formaldehyde for 18 weeks. 28 mal...

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Autores principales:	Golalipour,Mohammad Jafar, Azarhoush,Ramin, Ghafari,Soraya, Davarian,Ali, Fazeli,Seyyed Amirhossein
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2009
Materias:	Formaldehyde Exposure Histology Kidney Rat Morphometry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400037
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spelling	oai:scielo:S0717-950220090004000372010-05-18Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?Golalipour,Mohammad JafarAzarhoush,RaminGhafari,SorayaDavarian,AliFazeli,Seyyed Amirhossein Formaldehyde Exposure Histology Kidney Rat Morphometry Formaldehyde is used traditionally for fixing the cadaver, and vaporized during dissection and practical studying on cadaver. This study was designed to determine the histopathologic and morphometric changes of rat kidney while all of the experiments were exposed to formaldehyde for 18 weeks. 28 male albino Wistar rats were divided into the following three experimental groups (E1: 2hrs/d, 2d/w; E2: 2hrs/d, 4d/w; E3: 4hrs/d, 4d/w) and one control group (C). when the exposure period was expired the animals were anaesthetized with chloroform. After cervical dislocation, the abdomen was dissected and the kidneys were taken. The kidney specimens were sectioned and stained with Haematoxylin and Eosin technique for histologic and morphometric study. Data were obtained from an Olympus light microscope and the analyzed with spss (version 11.5) and ANOVA test. In all histopathology sections of groups E1, E2 and E3, these similar changes were seen: mild glumerolar congestion, focal congestion, and vacuolar degeneration of tubular cells. There were no evidences of inflammatory cells infiltration or fibrotic changes of interstitial tissue. Only mild, non-specific congestion was seen in cortical vessels. Also there were not any abnormalities in the staining of nucleus and cytoplasm. According to Morphometric study, Mean ±SD of glomerulus's area in control, E1, E2 and E3 group were 10802.66±1038.18, 10759.50±1971.88, 10434.73±1763.76 and 10077.64±2068.78 micrometer, respectively. Mean ±SD inner proximal tubule diameter in control, E1, E2 and E3 group were 16.16±2.49, 16.92±2.90, 16.31±2.79 and15.66±4.11 µm, respectively. Mean ±SD of inner distal tubule diameter in control, E1, E2 and E3 group were 15.96±4.47, 16.20±1.66, 16.96±1.63 and17.45±3.26 µm, respectively. These differences were not significant between cases and control. This study showed that formaldehyde inhalation in 1.5 ppm can not make specific Histopathologic and Morphometric changes in rat kidney.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.27 n.4 20092009-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400037en10.4067/S0717-95022009000400037
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Formaldehyde Exposure Histology Kidney Rat Morphometry
spellingShingle	Formaldehyde Exposure Histology Kidney Rat Morphometry Golalipour,Mohammad Jafar Azarhoush,Ramin Ghafari,Soraya Davarian,Ali Fazeli,Seyyed Amirhossein Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?
description	Formaldehyde is used traditionally for fixing the cadaver, and vaporized during dissection and practical studying on cadaver. This study was designed to determine the histopathologic and morphometric changes of rat kidney while all of the experiments were exposed to formaldehyde for 18 weeks. 28 male albino Wistar rats were divided into the following three experimental groups (E1: 2hrs/d, 2d/w; E2: 2hrs/d, 4d/w; E3: 4hrs/d, 4d/w) and one control group (C). when the exposure period was expired the animals were anaesthetized with chloroform. After cervical dislocation, the abdomen was dissected and the kidneys were taken. The kidney specimens were sectioned and stained with Haematoxylin and Eosin technique for histologic and morphometric study. Data were obtained from an Olympus light microscope and the analyzed with spss (version 11.5) and ANOVA test. In all histopathology sections of groups E1, E2 and E3, these similar changes were seen: mild glumerolar congestion, focal congestion, and vacuolar degeneration of tubular cells. There were no evidences of inflammatory cells infiltration or fibrotic changes of interstitial tissue. Only mild, non-specific congestion was seen in cortical vessels. Also there were not any abnormalities in the staining of nucleus and cytoplasm. According to Morphometric study, Mean ±SD of glomerulus's area in control, E1, E2 and E3 group were 10802.66±1038.18, 10759.50±1971.88, 10434.73±1763.76 and 10077.64±2068.78 micrometer, respectively. Mean ±SD inner proximal tubule diameter in control, E1, E2 and E3 group were 16.16±2.49, 16.92±2.90, 16.31±2.79 and15.66±4.11 µm, respectively. Mean ±SD of inner distal tubule diameter in control, E1, E2 and E3 group were 15.96±4.47, 16.20±1.66, 16.96±1.63 and17.45±3.26 µm, respectively. These differences were not significant between cases and control. This study showed that formaldehyde inhalation in 1.5 ppm can not make specific Histopathologic and Morphometric changes in rat kidney.
author	Golalipour,Mohammad Jafar Azarhoush,Ramin Ghafari,Soraya Davarian,Ali Fazeli,Seyyed Amirhossein
author_facet	Golalipour,Mohammad Jafar Azarhoush,Ramin Ghafari,Soraya Davarian,Ali Fazeli,Seyyed Amirhossein
author_sort	Golalipour,Mohammad Jafar
title	Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?
title_short	Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?
title_full	Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?
title_fullStr	Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?
title_full_unstemmed	Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?
title_sort	can formaldehyde exposure induce histopathologic and morphometric changes on rat kidney?
publisher	Sociedad Chilena de Anatomía
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400037
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Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?

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