Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum

The ultimate goal of periodontal therapy is to repair the damaged periodontal supporting tissues, permitting regeneration of the periodontal ligament. However, the cell response, the supportive matrix and the bioactive molecules use have not yet been well established. Bone marrow mononuclear cells w...

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Autores principales:	Milagres,Débora, Rueff-Barroso,Carlos R, Carvalho,Simone N, Carvalho,Laís de, Monte-Alto-Costa,Andréa, Fischer,Ricardo G, Porto,Luís Cristóvão
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2009
Materias:	Stem cells Collagen membrane Bone graft Cementum Proliferation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400045
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spelling	oai:scielo:S0717-950220090004000452010-05-18Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth CementumMilagres,DéboraRueff-Barroso,Carlos RCarvalho,Simone NCarvalho,Laís deMonte-Alto-Costa,AndréaFischer,Ricardo GPorto,Luís Cristóvão Stem cells Collagen membrane Bone graft Cementum Proliferation The ultimate goal of periodontal therapy is to repair the damaged periodontal supporting tissues, permitting regeneration of the periodontal ligament. However, the cell response, the supportive matrix and the bioactive molecules use have not yet been well established. Bone marrow mononuclear cells were extracted from rat femurs and tibiae and cultured on a cross-linked collagen membrane, bone graft, or molar tooth to compare cell attachment and early proliferation on these materials. Cell attachment was quantified by light microscopy at 24, 48 and 72h, and cell proliferation was observed under a SEM after 72h. After 24h, the number of cells on bone graft was similar to that of the control and more than twice compared to collagen membrane (q=7.473 p<0.001) and 1.75 times greater than with tooth cementum (q=5.613 p<0.01). However, the number of cells close to bone graft decreased in the second day compared to the control. SEM examination revealed a significant decrease in the number of cells that attached and proliferated on tooth and bone graft when compared with membrane. The results showed that bone marrow mesenchymal cells offer great potential for colonize a collagen membrane.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.27 n.4 20092009-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400045en10.4067/S0717-95022009000400045
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Stem cells Collagen membrane Bone graft Cementum Proliferation
spellingShingle	Stem cells Collagen membrane Bone graft Cementum Proliferation Milagres,Débora Rueff-Barroso,Carlos R Carvalho,Simone N Carvalho,Laís de Monte-Alto-Costa,Andréa Fischer,Ricardo G Porto,Luís Cristóvão Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum
description	The ultimate goal of periodontal therapy is to repair the damaged periodontal supporting tissues, permitting regeneration of the periodontal ligament. However, the cell response, the supportive matrix and the bioactive molecules use have not yet been well established. Bone marrow mononuclear cells were extracted from rat femurs and tibiae and cultured on a cross-linked collagen membrane, bone graft, or molar tooth to compare cell attachment and early proliferation on these materials. Cell attachment was quantified by light microscopy at 24, 48 and 72h, and cell proliferation was observed under a SEM after 72h. After 24h, the number of cells on bone graft was similar to that of the control and more than twice compared to collagen membrane (q=7.473 p<0.001) and 1.75 times greater than with tooth cementum (q=5.613 p<0.01). However, the number of cells close to bone graft decreased in the second day compared to the control. SEM examination revealed a significant decrease in the number of cells that attached and proliferated on tooth and bone graft when compared with membrane. The results showed that bone marrow mesenchymal cells offer great potential for colonize a collagen membrane.
author	Milagres,Débora Rueff-Barroso,Carlos R Carvalho,Simone N Carvalho,Laís de Monte-Alto-Costa,Andréa Fischer,Ricardo G Porto,Luís Cristóvão
author_facet	Milagres,Débora Rueff-Barroso,Carlos R Carvalho,Simone N Carvalho,Laís de Monte-Alto-Costa,Andréa Fischer,Ricardo G Porto,Luís Cristóvão
author_sort	Milagres,Débora
title	Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum
title_short	Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum
title_full	Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum
title_fullStr	Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum
title_full_unstemmed	Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum
title_sort	early proliferation of bone marrow mononuclear cells on collagen membrane, bone graft and tooth cementum
publisher	Sociedad Chilena de Anatomía
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400045
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Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum

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