Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin: I Cauda Epididymal Spermatozoa

At present it is not clear if male fertility is affected by intermittent hypobaric hypoxia (IHH). This is an important issue since a large human population works over 3000 masl. This study analyzes epididymal sperm, in adult Sprague Dawley rats after five cycles of IHH (7 days exposure to 4200 masl...

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Autores principales:	Hartley,Ricardo, Castro-Sánchez,Rodrigo, Ramos-Gonzalez,Benito, Bustos-Obregón,Eduardo
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2009
Materias:	Sperm quality Intermittent hypobaric hypoxia Melatonin protection
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400049
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spelling	oai:scielo:S0717-950220090004000492010-05-18Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin: I Cauda Epididymal SpermatozoaHartley,RicardoCastro-Sánchez,RodrigoRamos-Gonzalez,BenitoBustos-Obregón,Eduardo Sperm quality Intermittent hypobaric hypoxia Melatonin protection At present it is not clear if male fertility is affected by intermittent hypobaric hypoxia (IHH). This is an important issue since a large human population works over 3000 masl. This study analyzes epididymal sperm, in adult Sprague Dawley rats after five cycles of IHH (7 days exposure to 4200 masl in a hypobaric chamber / 7 days at 500 masl). The animals were separated into groups of 8, one group was exposed to hypoxia (7 days), and the others to IHH for one to five cycles. Controls (500 masl) were examined at the beginning and at the end of the 70 experimental days. A duplicate set of rats treated with melatonin (supposedly protecting from hypoxia) was also examined, as were their controls, injected with 0,03% ethanol (melatonin solvent). Epididymal sperm parameters, were evaluated. Damage caused by IHH increases with time. Sperm counts drop, while sperm chromatin swelling, DNA instability (metachromasia with acridine orange epifluorescence) and comet (+) tests increase. Melatonin counteracts all this damage, possibly due to its high efficiency as a reactive oxygen species scavenger. In conclusion, IHH exposure damages sperm quality and therefore male reproductive function.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.27 n.4 20092009-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400049en10.4067/S0717-95022009000400049
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Sperm quality Intermittent hypobaric hypoxia Melatonin protection
spellingShingle	Sperm quality Intermittent hypobaric hypoxia Melatonin protection Hartley,Ricardo Castro-Sánchez,Rodrigo Ramos-Gonzalez,Benito Bustos-Obregón,Eduardo Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin: I Cauda Epididymal Spermatozoa
description	At present it is not clear if male fertility is affected by intermittent hypobaric hypoxia (IHH). This is an important issue since a large human population works over 3000 masl. This study analyzes epididymal sperm, in adult Sprague Dawley rats after five cycles of IHH (7 days exposure to 4200 masl in a hypobaric chamber / 7 days at 500 masl). The animals were separated into groups of 8, one group was exposed to hypoxia (7 days), and the others to IHH for one to five cycles. Controls (500 masl) were examined at the beginning and at the end of the 70 experimental days. A duplicate set of rats treated with melatonin (supposedly protecting from hypoxia) was also examined, as were their controls, injected with 0,03% ethanol (melatonin solvent). Epididymal sperm parameters, were evaluated. Damage caused by IHH increases with time. Sperm counts drop, while sperm chromatin swelling, DNA instability (metachromasia with acridine orange epifluorescence) and comet (+) tests increase. Melatonin counteracts all this damage, possibly due to its high efficiency as a reactive oxygen species scavenger. In conclusion, IHH exposure damages sperm quality and therefore male reproductive function.
author	Hartley,Ricardo Castro-Sánchez,Rodrigo Ramos-Gonzalez,Benito Bustos-Obregón,Eduardo
author_facet	Hartley,Ricardo Castro-Sánchez,Rodrigo Ramos-Gonzalez,Benito Bustos-Obregón,Eduardo
author_sort	Hartley,Ricardo
title	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin: I Cauda Epididymal Spermatozoa
title_short	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin: I Cauda Epididymal Spermatozoa
title_full	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin: I Cauda Epididymal Spermatozoa
title_fullStr	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin: I Cauda Epididymal Spermatozoa
title_full_unstemmed	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin: I Cauda Epididymal Spermatozoa
title_sort	rat spermatogenesis damage in intermittent hypobaric hypoxia and the protective role of melatonin: i cauda epididymal spermatozoa
publisher	Sociedad Chilena de Anatomía
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400049
work_keys_str_mv	AT hartleyricardo ratspermatogenesisdamageinintermittenthypobarichypoxiaandtheprotectiveroleofmelatoninicaudaepididymalspermatozoa AT castrosanchezrodrigo ratspermatogenesisdamageinintermittenthypobarichypoxiaandtheprotectiveroleofmelatoninicaudaepididymalspermatozoa AT ramosgonzalezbenito ratspermatogenesisdamageinintermittenthypobarichypoxiaandtheprotectiveroleofmelatoninicaudaepididymalspermatozoa AT bustosobregoneduardo ratspermatogenesisdamageinintermittenthypobarichypoxiaandtheprotectiveroleofmelatoninicaudaepididymalspermatozoa
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Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin: I Cauda Epididymal Spermatozoa

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