Correlation Between Function-Morphology of Effect of the Stress and Flunitrazepam Administered During Gestation in Adult Mouse Ovary

Previously we report long lasting effects on ovary of mice prenatally exposed to flunitrazepam (FNZ), a benzodiazepine with tranquilized action. In this work we find that the FNZ don't prevent the effects on ovary prenatally exposure to stress in mice. We studied adult females born from mothers...

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Autores principales:	Aguirre-Samudio,Ana Julia, de la Fuente-Juáre,Graciela, Márquez-Orozco,Amalia, Márquez-Orozco,María Cristina
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2010
Materias:	Benzodiazepine 3 b-HSD Flunitrazepam Mouse Ovaries Atretics follicles Prenatal stress
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100045
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spelling	oai:scielo:S0717-950220100001000452010-07-05Correlation Between Function-Morphology of Effect of the Stress and Flunitrazepam Administered During Gestation in Adult Mouse OvaryAguirre-Samudio,Ana Juliade la Fuente-Juáre,GracielaMárquez-Orozco,AmaliaMárquez-Orozco,María Cristina Benzodiazepine 3 b-HSD Flunitrazepam Mouse Ovaries Atretics follicles Prenatal stress Previously we report long lasting effects on ovary of mice prenatally exposed to flunitrazepam (FNZ), a benzodiazepine with tranquilized action. In this work we find that the FNZ don't prevent the effects on ovary prenatally exposure to stress in mice. We studied adult females born from mothers that had been stressed by immobilization on day 6 ofgestation (GD-6) or group S, and from mothers stressed also by immobilization at GD-6, but which received a single oral dose of FNZ immediately after the stress group FNZS. The control groups were the SS that received the GD-6 saline solution and the group NT non-treated. Their ovaries were extracted for histology studies and to observe the activity of 3b hydroxysteroid dehydrogenase/isomerase (3 b-HSD). The histological analysis revealed high staining affinity ovarian cell of S and FNZS. Double oocytes and apoptotic bodies were found in the secondary atretic follicles, as well as abnormal primordial, primary and secondary follicle populations, as compared to SS and NT groups. The primordial, primary, and secondary follicles were significantly reduced in the experimental groups. But the primary and secondary atretic follicles were higher in both groups, and the number of corpora lutea was lower in both groups. The activity of 3 b-HSD was abnormally increased in both FNZS- and S-groups. These findings suggest that FNZ did not counteract the impairing effects of prenatal stress on adult offspring ovarian follicles, and could rather be responsible for long lasting changes occurring during embryonic programming.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.28 n.1 20102010-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100045en10.4067/S0717-95022010000100045
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Benzodiazepine 3 b-HSD Flunitrazepam Mouse Ovaries Atretics follicles Prenatal stress
spellingShingle	Benzodiazepine 3 b-HSD Flunitrazepam Mouse Ovaries Atretics follicles Prenatal stress Aguirre-Samudio,Ana Julia de la Fuente-Juáre,Graciela Márquez-Orozco,Amalia Márquez-Orozco,María Cristina Correlation Between Function-Morphology of Effect of the Stress and Flunitrazepam Administered During Gestation in Adult Mouse Ovary
description	Previously we report long lasting effects on ovary of mice prenatally exposed to flunitrazepam (FNZ), a benzodiazepine with tranquilized action. In this work we find that the FNZ don't prevent the effects on ovary prenatally exposure to stress in mice. We studied adult females born from mothers that had been stressed by immobilization on day 6 ofgestation (GD-6) or group S, and from mothers stressed also by immobilization at GD-6, but which received a single oral dose of FNZ immediately after the stress group FNZS. The control groups were the SS that received the GD-6 saline solution and the group NT non-treated. Their ovaries were extracted for histology studies and to observe the activity of 3b hydroxysteroid dehydrogenase/isomerase (3 b-HSD). The histological analysis revealed high staining affinity ovarian cell of S and FNZS. Double oocytes and apoptotic bodies were found in the secondary atretic follicles, as well as abnormal primordial, primary and secondary follicle populations, as compared to SS and NT groups. The primordial, primary, and secondary follicles were significantly reduced in the experimental groups. But the primary and secondary atretic follicles were higher in both groups, and the number of corpora lutea was lower in both groups. The activity of 3 b-HSD was abnormally increased in both FNZS- and S-groups. These findings suggest that FNZ did not counteract the impairing effects of prenatal stress on adult offspring ovarian follicles, and could rather be responsible for long lasting changes occurring during embryonic programming.
author	Aguirre-Samudio,Ana Julia de la Fuente-Juáre,Graciela Márquez-Orozco,Amalia Márquez-Orozco,María Cristina
author_facet	Aguirre-Samudio,Ana Julia de la Fuente-Juáre,Graciela Márquez-Orozco,Amalia Márquez-Orozco,María Cristina
author_sort	Aguirre-Samudio,Ana Julia
title	Correlation Between Function-Morphology of Effect of the Stress and Flunitrazepam Administered During Gestation in Adult Mouse Ovary
title_short	Correlation Between Function-Morphology of Effect of the Stress and Flunitrazepam Administered During Gestation in Adult Mouse Ovary
title_full	Correlation Between Function-Morphology of Effect of the Stress and Flunitrazepam Administered During Gestation in Adult Mouse Ovary
title_fullStr	Correlation Between Function-Morphology of Effect of the Stress and Flunitrazepam Administered During Gestation in Adult Mouse Ovary
title_full_unstemmed	Correlation Between Function-Morphology of Effect of the Stress and Flunitrazepam Administered During Gestation in Adult Mouse Ovary
title_sort	correlation between function-morphology of effect of the stress and flunitrazepam administered during gestation in adult mouse ovary
publisher	Sociedad Chilena de Anatomía
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100045
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Correlation Between Function-Morphology of Effect of the Stress and Flunitrazepam Administered During Gestation in Adult Mouse Ovary

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