Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus

The present study was planned to demonstrate the detailed immunoreactive (IR) distribution pattern of estrogen receptors (ER) in hippocampus of 15 female rats, adult, female Wistar rats in estrous phase. 30 µm thick setions of hippocampal region fixed (4% buffered paraformaldehyde) were obtained wit...

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Autores principales: Rai,Anju Lata, Jeswar,Usha
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2010
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200024
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spelling oai:scielo:S0717-950220100002000242010-10-21Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat HippocampusRai,Anju LataJeswar,Usha Estrogen receptor-a Estrogen receptor-b Brain Hippocampus Interneurons Cornua ammonis The present study was planned to demonstrate the detailed immunoreactive (IR) distribution pattern of estrogen receptors (ER) in hippocampus of 15 female rats, adult, female Wistar rats in estrous phase. 30 µm thick setions of hippocampal region fixed (4% buffered paraformaldehyde) were obtained with cryostat. The sections were processed free- floating for immunolocalization of ER using, mouse monoclonal anti-ER-a antibody with PAP technique. The results showed presence of ER immunoreactive neurons in all the subfields of hippocampus with some variations. In cornua ammonis (CA) maximum ER positive (+ve) neurons were localized in CA3 region. Layer analysis showed maximum localization in the stratum oriens (SO) region. In other subfields and layers of CA the IR neurons were comparatively less in number. The morphological characters of all ER +ve neurons showed them to be interneurons both in CA as well as in Dentate gyrus (DG).info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.28 n.2 20102010-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200024en10.4067/S0717-95022010000200024
institution Scielo Chile
collection Scielo Chile
language English
topic Estrogen receptor-a
Estrogen receptor-b
Brain
Hippocampus
Interneurons
Cornua ammonis
spellingShingle Estrogen receptor-a
Estrogen receptor-b
Brain
Hippocampus
Interneurons
Cornua ammonis
Rai,Anju Lata
Jeswar,Usha
Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus
description The present study was planned to demonstrate the detailed immunoreactive (IR) distribution pattern of estrogen receptors (ER) in hippocampus of 15 female rats, adult, female Wistar rats in estrous phase. 30 µm thick setions of hippocampal region fixed (4% buffered paraformaldehyde) were obtained with cryostat. The sections were processed free- floating for immunolocalization of ER using, mouse monoclonal anti-ER-a antibody with PAP technique. The results showed presence of ER immunoreactive neurons in all the subfields of hippocampus with some variations. In cornua ammonis (CA) maximum ER positive (+ve) neurons were localized in CA3 region. Layer analysis showed maximum localization in the stratum oriens (SO) region. In other subfields and layers of CA the IR neurons were comparatively less in number. The morphological characters of all ER +ve neurons showed them to be interneurons both in CA as well as in Dentate gyrus (DG).
author Rai,Anju Lata
Jeswar,Usha
author_facet Rai,Anju Lata
Jeswar,Usha
author_sort Rai,Anju Lata
title Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus
title_short Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus
title_full Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus
title_fullStr Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus
title_full_unstemmed Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus
title_sort immunolocalization of estrogen receptor alpha in adult female rat hippocampus
publisher Sociedad Chilena de Anatomía
publishDate 2010
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200024
work_keys_str_mv AT raianjulata immunolocalizationofestrogenreceptoralphainadultfemalerathippocampus
AT jeswarusha immunolocalizationofestrogenreceptoralphainadultfemalerathippocampus
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