Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus
The present study was planned to demonstrate the detailed immunoreactive (IR) distribution pattern of estrogen receptors (ER) in hippocampus of 15 female rats, adult, female Wistar rats in estrous phase. 30 µm thick setions of hippocampal region fixed (4% buffered paraformaldehyde) were obtained wit...
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Sociedad Chilena de Anatomía
2010
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oai:scielo:S0717-950220100002000242010-10-21Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat HippocampusRai,Anju LataJeswar,Usha Estrogen receptor-a Estrogen receptor-b Brain Hippocampus Interneurons Cornua ammonis The present study was planned to demonstrate the detailed immunoreactive (IR) distribution pattern of estrogen receptors (ER) in hippocampus of 15 female rats, adult, female Wistar rats in estrous phase. 30 µm thick setions of hippocampal region fixed (4% buffered paraformaldehyde) were obtained with cryostat. The sections were processed free- floating for immunolocalization of ER using, mouse monoclonal anti-ER-a antibody with PAP technique. The results showed presence of ER immunoreactive neurons in all the subfields of hippocampus with some variations. In cornua ammonis (CA) maximum ER positive (+ve) neurons were localized in CA3 region. Layer analysis showed maximum localization in the stratum oriens (SO) region. In other subfields and layers of CA the IR neurons were comparatively less in number. The morphological characters of all ER +ve neurons showed them to be interneurons both in CA as well as in Dentate gyrus (DG).info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.28 n.2 20102010-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200024en10.4067/S0717-95022010000200024 |
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Scielo Chile |
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Scielo Chile |
language |
English |
topic |
Estrogen receptor-a Estrogen receptor-b Brain Hippocampus Interneurons Cornua ammonis |
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Estrogen receptor-a Estrogen receptor-b Brain Hippocampus Interneurons Cornua ammonis Rai,Anju Lata Jeswar,Usha Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus |
description |
The present study was planned to demonstrate the detailed immunoreactive (IR) distribution pattern of estrogen receptors (ER) in hippocampus of 15 female rats, adult, female Wistar rats in estrous phase. 30 µm thick setions of hippocampal region fixed (4% buffered paraformaldehyde) were obtained with cryostat. The sections were processed free- floating for immunolocalization of ER using, mouse monoclonal anti-ER-a antibody with PAP technique. The results showed presence of ER immunoreactive neurons in all the subfields of hippocampus with some variations. In cornua ammonis (CA) maximum ER positive (+ve) neurons were localized in CA3 region. Layer analysis showed maximum localization in the stratum oriens (SO) region. In other subfields and layers of CA the IR neurons were comparatively less in number. The morphological characters of all ER +ve neurons showed them to be interneurons both in CA as well as in Dentate gyrus (DG). |
author |
Rai,Anju Lata Jeswar,Usha |
author_facet |
Rai,Anju Lata Jeswar,Usha |
author_sort |
Rai,Anju Lata |
title |
Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus |
title_short |
Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus |
title_full |
Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus |
title_fullStr |
Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus |
title_full_unstemmed |
Immunolocalization of Estrogen Receptor Alpha in Adult Female Rat Hippocampus |
title_sort |
immunolocalization of estrogen receptor alpha in adult female rat hippocampus |
publisher |
Sociedad Chilena de Anatomía |
publishDate |
2010 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200024 |
work_keys_str_mv |
AT raianjulata immunolocalizationofestrogenreceptoralphainadultfemalerathippocampus AT jeswarusha immunolocalizationofestrogenreceptoralphainadultfemalerathippocampus |
_version_ |
1718444693689204736 |