Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition

The effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. Six 90-day-old Wistar rats (Rattus norvegicus) were assigned into two groups: CG (Control Group, n = 3) and MG (Malnourished Group, n = 3). CG animals were...

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Autores principales:	Franco,Cássia Luiza de Marins, Sant'Ana,Débora de Mello Gonçales, Araújo,Eduardo José de Almeida
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2010
Materias:	Protein-energetic malnutrition Small intestine Morphometry Mucins
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200026
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spelling	oai:scielo:S0717-950220100002000262010-10-21Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein MalnutritionFranco,Cássia Luiza de MarinsSant'Ana,Débora de Mello GonçalesAraújo,Eduardo José de Almeida Protein-energetic malnutrition Small intestine Morphometry Mucins The effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. Six 90-day-old Wistar rats (Rattus norvegicus) were assigned into two groups: CG (Control Group, n = 3) and MG (Malnourished Group, n = 3). CG animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. After 90 days, part of the jejunum was collected and subjected to routine histology. HE-staining and histochemical techniques - Periodic Acid Schiff (PAS) and Alcian Blue (AB), pH 2.5/pH 1.0 - were used for the detection of glycoconjugates in 4µm -thick transverse cuts. Morphometric analysis of the HE-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the following layers: external muscle and mucosa - enterocyte height also decreased. Cuts stained by using histochemical techniques for the detection of glycoconjugates revealed maintenance of goblet cells reactive to PAS and AB pH 2.5, whereas the number of cells reactive to AB pH 1.0 increased. Protein malnutrition was concluded to provoke severe atrophy of the jejunal wall and an increase of sulphomucin secretion within the intestinal epithelium.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.28 n.2 20102010-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200026en10.4067/S0717-95022010000200026
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Protein-energetic malnutrition Small intestine Morphometry Mucins
spellingShingle	Protein-energetic malnutrition Small intestine Morphometry Mucins Franco,Cássia Luiza de Marins Sant'Ana,Débora de Mello Gonçales Araújo,Eduardo José de Almeida Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition
description	The effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. Six 90-day-old Wistar rats (Rattus norvegicus) were assigned into two groups: CG (Control Group, n = 3) and MG (Malnourished Group, n = 3). CG animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. After 90 days, part of the jejunum was collected and subjected to routine histology. HE-staining and histochemical techniques - Periodic Acid Schiff (PAS) and Alcian Blue (AB), pH 2.5/pH 1.0 - were used for the detection of glycoconjugates in 4µm -thick transverse cuts. Morphometric analysis of the HE-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the following layers: external muscle and mucosa - enterocyte height also decreased. Cuts stained by using histochemical techniques for the detection of glycoconjugates revealed maintenance of goblet cells reactive to PAS and AB pH 2.5, whereas the number of cells reactive to AB pH 1.0 increased. Protein malnutrition was concluded to provoke severe atrophy of the jejunal wall and an increase of sulphomucin secretion within the intestinal epithelium.
author	Franco,Cássia Luiza de Marins Sant'Ana,Débora de Mello Gonçales Araújo,Eduardo José de Almeida
author_facet	Franco,Cássia Luiza de Marins Sant'Ana,Débora de Mello Gonçales Araújo,Eduardo José de Almeida
author_sort	Franco,Cássia Luiza de Marins
title	Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition
title_short	Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition
title_full	Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition
title_fullStr	Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition
title_full_unstemmed	Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition
title_sort	intestinal wall atrophy and increase of sulphomucin secretion in the jejunal epithelium of rats submitted to severe protein malnutrition
publisher	Sociedad Chilena de Anatomía
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200026
work_keys_str_mv	AT francocassialuizademarins intestinalwallatrophyandincreaseofsulphomucinsecretioninthejejunalepitheliumofratssubmittedtosevereproteinmalnutrition AT santanadeborademellogoncales intestinalwallatrophyandincreaseofsulphomucinsecretioninthejejunalepitheliumofratssubmittedtosevereproteinmalnutrition AT araujoeduardojosedealmeida intestinalwallatrophyandincreaseofsulphomucinsecretioninthejejunalepitheliumofratssubmittedtosevereproteinmalnutrition
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Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition

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