Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin. II: Testicular Parameters

At present it is not clear if male fertility is affected by intermittent hypobaric hypoxia (IHH). This is an important issue since a large human population works over 3000 masl. This study analyzes testicular changes in adult Sprague Dawley rats after five cycles of IHH (7 day exposure to 4200 masl...

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Autores principales:	Bustos-Obregón,Eduardo, Castro-Sánchez,Rodrigo, Ramos-González,Benito, Torres-Díaz,Leandro
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2010
Materias:	Spermatogenesis intermittent hypobaric hypoxia melatonin protection
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200034
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spelling	oai:scielo:S0717-950220100002000342010-10-21Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin. II: Testicular ParametersBustos-Obregón,EduardoCastro-Sánchez,RodrigoRamos-González,BenitoTorres-Díaz,Leandro Spermatogenesis intermittent hypobaric hypoxia melatonin protection At present it is not clear if male fertility is affected by intermittent hypobaric hypoxia (IHH). This is an important issue since a large human population works over 3000 masl. This study analyzes testicular changes in adult Sprague Dawley rats after five cycles of IHH (7 day exposure to 4200 masl in a hypobaric chamber / 7 day at 500 masl). The animals were separated into groups of 8, one group was exposed to hypoxia (7 days), and the others to IHH for one to five cycles. Controls (500 masl) were examined at the beginning and at the end of the 70 experimental days. A duplicate set of rats treated with melatonin (supposedly protecting from hypoxia) was also examined, as were their controls, injected with 0,03% ethanol (melatonin solvent).Immunohistochemical and histometric analysis of testicular tissue were performed. Damage caused by IHH increases with time. Morphometry reveals an increase in tubular and luminal diameters and a reduction in epithelial height. Inmunohistochemistry for HIF-1alpha shows an increase with time, however the opposite happens with HSP-70. Spermatogenic cells submitted to comet assay present an increase of (+) cells. Melatonin counteracts all this damage, possibly due to its high efficiency as a reactive oxygen species scavenger. In conclusion, IHH exposure damages male reproductive function.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.28 n.2 20102010-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200034en10.4067/S0717-95022010000200034
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Spermatogenesis intermittent hypobaric hypoxia melatonin protection
spellingShingle	Spermatogenesis intermittent hypobaric hypoxia melatonin protection Bustos-Obregón,Eduardo Castro-Sánchez,Rodrigo Ramos-González,Benito Torres-Díaz,Leandro Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin. II: Testicular Parameters
description	At present it is not clear if male fertility is affected by intermittent hypobaric hypoxia (IHH). This is an important issue since a large human population works over 3000 masl. This study analyzes testicular changes in adult Sprague Dawley rats after five cycles of IHH (7 day exposure to 4200 masl in a hypobaric chamber / 7 day at 500 masl). The animals were separated into groups of 8, one group was exposed to hypoxia (7 days), and the others to IHH for one to five cycles. Controls (500 masl) were examined at the beginning and at the end of the 70 experimental days. A duplicate set of rats treated with melatonin (supposedly protecting from hypoxia) was also examined, as were their controls, injected with 0,03% ethanol (melatonin solvent).Immunohistochemical and histometric analysis of testicular tissue were performed. Damage caused by IHH increases with time. Morphometry reveals an increase in tubular and luminal diameters and a reduction in epithelial height. Inmunohistochemistry for HIF-1alpha shows an increase with time, however the opposite happens with HSP-70. Spermatogenic cells submitted to comet assay present an increase of (+) cells. Melatonin counteracts all this damage, possibly due to its high efficiency as a reactive oxygen species scavenger. In conclusion, IHH exposure damages male reproductive function.
author	Bustos-Obregón,Eduardo Castro-Sánchez,Rodrigo Ramos-González,Benito Torres-Díaz,Leandro
author_facet	Bustos-Obregón,Eduardo Castro-Sánchez,Rodrigo Ramos-González,Benito Torres-Díaz,Leandro
author_sort	Bustos-Obregón,Eduardo
title	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin. II: Testicular Parameters
title_short	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin. II: Testicular Parameters
title_full	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin. II: Testicular Parameters
title_fullStr	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin. II: Testicular Parameters
title_full_unstemmed	Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin. II: Testicular Parameters
title_sort	rat spermatogenesis damage in intermittent hypobaric hypoxia and the protective role of melatonin. ii: testicular parameters
publisher	Sociedad Chilena de Anatomía
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200034
work_keys_str_mv	AT bustosobregoneduardo ratspermatogenesisdamageinintermittenthypobarichypoxiaandtheprotectiveroleofmelatoniniitesticularparameters AT castrosanchezrodrigo ratspermatogenesisdamageinintermittenthypobarichypoxiaandtheprotectiveroleofmelatoniniitesticularparameters AT ramosgonzalezbenito ratspermatogenesisdamageinintermittenthypobarichypoxiaandtheprotectiveroleofmelatoniniitesticularparameters AT torresdiazleandro ratspermatogenesisdamageinintermittenthypobarichypoxiaandtheprotectiveroleofmelatoniniitesticularparameters
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Rat Spermatogenesis Damage in Intermittent Hypobaric Hypoxia and the Protective Role of Melatonin. II: Testicular Parameters

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