Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells

Although the structure and the functions of juxtaglomerular cells (JG) have been well defined, there is still a controversy about the secretory mechanisms of renin from these cells. It has been assumed that exocytosis is the main secretory mechanism in these cells in many studies, while others sugge...

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Autores principales:	Aktas,Ranan Gulhan, Karabay,Gulten, Taskinalp,Oguz, Kutlu,A. Kemal
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2010
Materias:	Juxtaglomerular cells Secretory granules Microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300011
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spelling	oai:scielo:S0717-950220100003000112010-10-25Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular CellsAktas,Ranan GulhanKarabay,GultenTaskinalp,OguzKutlu,A. Kemal Juxtaglomerular cells Secretory granules Microscopy Although the structure and the functions of juxtaglomerular cells (JG) have been well defined, there is still a controversy about the secretory mechanisms of renin from these cells. It has been assumed that exocytosis is the main secretory mechanism in these cells in many studies, while others suggest that secretion occurs in a quite different way in these cells. There are several studies suggesting that diacrine secretion, which is very difficult to visualize, might be the other mechanism for secretion of renin. This study is an attempt to find the answers of these questions by identifying the fine structural features of the secretory granules in juxtaglomerular cells. Cyclosporin A (CyA) has been used in the current experimental study since it has already been reported that this drug increases the number of JG cells and stimulates secretion of Renin. Twelve female Sprague-Dawley rats had daily intraperitoneal injections of CyA for ten weeks. Tissue specimens from the kidneys of these animals were examined by electron microscopy. Fine structural characteristics of the secretory granules of juxtaglomerular cells have been examined. Considerable amount of granules, which goes to the exocytotic process, have been observed. Additionally, several cells, which their granules had been secreting their contents in a different way, were found. This was interpreted as the secretion type of diacrine secretion. In conclusion, this in vivo study presents morphologic evidences demonstrating that both exocytosis and diacrine secretion might occur in JG cells. We also had a chance to observe secretory granule probably exhibiting "diacrine secretion", which is very difficult to visualize, at electron microscope level for the first time. This report also provides morphologic proof which shows that these two distinct secretory mechanisms might occur simultaneously in the same juxtaglomerular cell.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.28 n.3 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300011en10.4067/S0717-95022010000300011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Juxtaglomerular cells Secretory granules Microscopy
spellingShingle	Juxtaglomerular cells Secretory granules Microscopy Aktas,Ranan Gulhan Karabay,Gulten Taskinalp,Oguz Kutlu,A. Kemal Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells
description	Although the structure and the functions of juxtaglomerular cells (JG) have been well defined, there is still a controversy about the secretory mechanisms of renin from these cells. It has been assumed that exocytosis is the main secretory mechanism in these cells in many studies, while others suggest that secretion occurs in a quite different way in these cells. There are several studies suggesting that diacrine secretion, which is very difficult to visualize, might be the other mechanism for secretion of renin. This study is an attempt to find the answers of these questions by identifying the fine structural features of the secretory granules in juxtaglomerular cells. Cyclosporin A (CyA) has been used in the current experimental study since it has already been reported that this drug increases the number of JG cells and stimulates secretion of Renin. Twelve female Sprague-Dawley rats had daily intraperitoneal injections of CyA for ten weeks. Tissue specimens from the kidneys of these animals were examined by electron microscopy. Fine structural characteristics of the secretory granules of juxtaglomerular cells have been examined. Considerable amount of granules, which goes to the exocytotic process, have been observed. Additionally, several cells, which their granules had been secreting their contents in a different way, were found. This was interpreted as the secretion type of diacrine secretion. In conclusion, this in vivo study presents morphologic evidences demonstrating that both exocytosis and diacrine secretion might occur in JG cells. We also had a chance to observe secretory granule probably exhibiting "diacrine secretion", which is very difficult to visualize, at electron microscope level for the first time. This report also provides morphologic proof which shows that these two distinct secretory mechanisms might occur simultaneously in the same juxtaglomerular cell.
author	Aktas,Ranan Gulhan Karabay,Gulten Taskinalp,Oguz Kutlu,A. Kemal
author_facet	Aktas,Ranan Gulhan Karabay,Gulten Taskinalp,Oguz Kutlu,A. Kemal
author_sort	Aktas,Ranan Gulhan
title	Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells
title_short	Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells
title_full	Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells
title_fullStr	Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells
title_full_unstemmed	Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells
title_sort	electron microscopic evaluation of the secretory mechanisms of renin from juxtaglomerular cells
publisher	Sociedad Chilena de Anatomía
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300011
work_keys_str_mv	AT aktasranangulhan electronmicroscopicevaluationofthesecretorymechanismsofreninfromjuxtaglomerularcells AT karabaygulten electronmicroscopicevaluationofthesecretorymechanismsofreninfromjuxtaglomerularcells AT taskinalpoguz electronmicroscopicevaluationofthesecretorymechanismsofreninfromjuxtaglomerularcells AT kutluakemal electronmicroscopicevaluationofthesecretorymechanismsofreninfromjuxtaglomerularcells
_version_	1718444700440985600

Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells

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