Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells: First Successful Latin-American Report

Osteoarthritis is the more frequent cause of disability in adult people and it is associated to cartilage degeneration of affected joints. This cartilage has a limited ability to repair. Several treatments have been tested including the use of Mesenchymal Stem Cells. These cells are an attractive so...

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Autores principales:	Mardones,Rodrigo, Martínez,Rafael, Paredes,María Jose, Zalaquett,Eugenio
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2010
Materias:	Chondrogenic differentiation Mesenchymal Stem Cells Osteoarthritis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300014
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spelling	oai:scielo:S0717-950220100003000142010-10-25Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells: First Successful Latin-American ReportMardones,RodrigoMartínez,RafaelParedes,María JoseZalaquett,Eugenio Chondrogenic differentiation Mesenchymal Stem Cells Osteoarthritis Osteoarthritis is the more frequent cause of disability in adult people and it is associated to cartilage degeneration of affected joints. This cartilage has a limited ability to repair. Several treatments have been tested including the use of Mesenchymal Stem Cells. These cells are an attractive source for cartilage repair because of their availability to chodrogenic differentiation by progressing sequentially through the expression of cartilage specific extracelullar matrix molecules, as in the embryologic human development. The aim is to obtain, culture and differentiate rabbit Bone Marrow derived Mesenchymal Stem Cells in vitro to chondral lineage. By differential centrifugation the mononuclear cell level was obtained from rabbit bone marrow samples. This level was cultured until 70% confluence. Chondrogenic differentiation was performed in an aggregate culture system with TGF-b1. Sample quantity, culture efficiency, confluence time of cultures and differentiation quality were all evaluated. An average sample of 14.5 ml per side was obtained, culture efficiency was 80%, and average confluence time (70%) was 18 days. Differentiation culture had an 80% efficiency and optimal differentiation quality. Rabbit Bone Marrow derived Mesenchymal Stem Cells culture is a reproducible technique and by the use of an adequate methodology chondrogenic cells can be obtained in vitro. This model permits the study of chondral differentiation process and could have direct clinical application. This is the first successful Latin-American report in Mesenchymal Stem Cells culture and chondrogenic differentiation.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.28 n.3 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300014en10.4067/S0717-95022010000300014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Chondrogenic differentiation Mesenchymal Stem Cells Osteoarthritis
spellingShingle	Chondrogenic differentiation Mesenchymal Stem Cells Osteoarthritis Mardones,Rodrigo Martínez,Rafael Paredes,María Jose Zalaquett,Eugenio Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells: First Successful Latin-American Report
description	Osteoarthritis is the more frequent cause of disability in adult people and it is associated to cartilage degeneration of affected joints. This cartilage has a limited ability to repair. Several treatments have been tested including the use of Mesenchymal Stem Cells. These cells are an attractive source for cartilage repair because of their availability to chodrogenic differentiation by progressing sequentially through the expression of cartilage specific extracelullar matrix molecules, as in the embryologic human development. The aim is to obtain, culture and differentiate rabbit Bone Marrow derived Mesenchymal Stem Cells in vitro to chondral lineage. By differential centrifugation the mononuclear cell level was obtained from rabbit bone marrow samples. This level was cultured until 70% confluence. Chondrogenic differentiation was performed in an aggregate culture system with TGF-b1. Sample quantity, culture efficiency, confluence time of cultures and differentiation quality were all evaluated. An average sample of 14.5 ml per side was obtained, culture efficiency was 80%, and average confluence time (70%) was 18 days. Differentiation culture had an 80% efficiency and optimal differentiation quality. Rabbit Bone Marrow derived Mesenchymal Stem Cells culture is a reproducible technique and by the use of an adequate methodology chondrogenic cells can be obtained in vitro. This model permits the study of chondral differentiation process and could have direct clinical application. This is the first successful Latin-American report in Mesenchymal Stem Cells culture and chondrogenic differentiation.
author	Mardones,Rodrigo Martínez,Rafael Paredes,María Jose Zalaquett,Eugenio
author_facet	Mardones,Rodrigo Martínez,Rafael Paredes,María Jose Zalaquett,Eugenio
author_sort	Mardones,Rodrigo
title	Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells: First Successful Latin-American Report
title_short	Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells: First Successful Latin-American Report
title_full	Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells: First Successful Latin-American Report
title_fullStr	Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells: First Successful Latin-American Report
title_full_unstemmed	Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells: First Successful Latin-American Report
title_sort	chondrogenic differentiation of bone marrow mesenchymal stem cells: first successful latin-american report
publisher	Sociedad Chilena de Anatomía
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300014
work_keys_str_mv	AT mardonesrodrigo chondrogenicdifferentiationofbonemarrowmesenchymalstemcellsfirstsuccessfullatinamericanreport AT martinezrafael chondrogenicdifferentiationofbonemarrowmesenchymalstemcellsfirstsuccessfullatinamericanreport AT paredesmariajose chondrogenicdifferentiationofbonemarrowmesenchymalstemcellsfirstsuccessfullatinamericanreport AT zalaquetteugenio chondrogenicdifferentiationofbonemarrowmesenchymalstemcellsfirstsuccessfullatinamericanreport
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Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells: First Successful Latin-American Report

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