Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration

Leukocyte-endothelial cell interactions have a pivotal role in immune responses. In our study Ten adult wistar albino rats weighing 180-200 g were used to obtain tissue samples. The animals were sacrificed by decapitation under ether anesthesia. The lymph nodes of the animals were then quickly remov...

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Autores principales:	Deveci,Engin, Deveci,Ienay
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2010
Materias:	Leukocytes Margination Migration
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300037
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spelling	oai:scielo:S0717-950220100003000372010-10-25Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and MigrationDeveci,EnginDeveci,Ienay Leukocytes Margination Migration Leukocyte-endothelial cell interactions have a pivotal role in immune responses. In our study Ten adult wistar albino rats weighing 180-200 g were used to obtain tissue samples. The animals were sacrificed by decapitation under ether anesthesia. The lymph nodes of the animals were then quickly removed. Specimens were immersed in the zinc iodide-osmium tetroxide (ZIO) solutation by Niebauer et al. (1969) and kept in the dark for 2 hours in this solution from temperature for fixation/staining. Samples were processed according to routine plastic embedding tecnique. Semithin sections of 1µm thick were cut and stained with methilene blue-azure II for light microscopic examination. We used zinc iodide-osmium tetroxide staining tecnique to distinguish endothelial cell from leukocytes.Present data supports the understanding of this unique relationship as documented by figures.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.28 n.3 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300037en10.4067/S0717-95022010000300037
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Leukocytes Margination Migration
spellingShingle	Leukocytes Margination Migration Deveci,Engin Deveci,Ienay Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration
description	Leukocyte-endothelial cell interactions have a pivotal role in immune responses. In our study Ten adult wistar albino rats weighing 180-200 g were used to obtain tissue samples. The animals were sacrificed by decapitation under ether anesthesia. The lymph nodes of the animals were then quickly removed. Specimens were immersed in the zinc iodide-osmium tetroxide (ZIO) solutation by Niebauer et al. (1969) and kept in the dark for 2 hours in this solution from temperature for fixation/staining. Samples were processed according to routine plastic embedding tecnique. Semithin sections of 1µm thick were cut and stained with methilene blue-azure II for light microscopic examination. We used zinc iodide-osmium tetroxide staining tecnique to distinguish endothelial cell from leukocytes.Present data supports the understanding of this unique relationship as documented by figures.
author	Deveci,Engin Deveci,Ienay
author_facet	Deveci,Engin Deveci,Ienay
author_sort	Deveci,Engin
title	Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration
title_short	Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration
title_full	Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration
title_fullStr	Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration
title_full_unstemmed	Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration
title_sort	ultrastructural evidences for endothelial cell - leukocytes interactions: margination and migration
publisher	Sociedad Chilena de Anatomía
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300037
work_keys_str_mv	AT deveciengin ultrastructuralevidencesforendothelialcellleukocytesinteractionsmarginationandmigration AT deveciienay ultrastructuralevidencesforendothelialcellleukocytesinteractionsmarginationandmigration
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Ultrastructural Evidences for Endothelial Cell - Leukocytes Interactions: Margination and Migration

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