Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate karyometric and stereological alterations in the rat...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Sociedad Chilena de Anatomía
2010
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300038 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-95022010000300038 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-950220100003000382010-10-25Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological StudyMartins,Alex TadeuSantos,Fabiano de Sant´Ana dosScannavino,Fábio Luiz FerreiraPires,Juliana RicoZuza,Elizângela PartataJunior,João Armando PadovaniAzoubel,ReinaldoDi Mateo,Miguel Angel SalaLopes,Ruberval Armando Sodium cyclamate Exocrine pancreas Karyometry Stereology The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate karyometric and stereological alterations in the rat fetal pancreas resulting from the intraperitoneal administration of sodium cyclamate. The exocrine pancreas of ten fetuses of rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas; the length of the umbilical cords also were measured. After the laboratory processing, semi-seriated 6mm cuts stained with haematoxyline and eosine were performed. In seven karyometric parameters (major, minor, and medium diameters, volume, area, perimeter, and volume-area ratio), the increase was statistically significant in the treated group when compared with control group. Stereological parameters showed in the treated group a significant increase in the cellular volume and a significant reduction in the numerical cellular density. These results showed that the sodium cyclamate in pregnant rats led to retardation of fetal development and hypertrophy in the exocrine pancreas of the rat fetuses.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.28 n.3 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300038en10.4067/S0717-95022010000300038 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Sodium cyclamate Exocrine pancreas Karyometry Stereology |
| spellingShingle |
Sodium cyclamate Exocrine pancreas Karyometry Stereology Martins,Alex Tadeu Santos,Fabiano de Sant´Ana dos Scannavino,Fábio Luiz Ferreira Pires,Juliana Rico Zuza,Elizângela Partata Junior,João Armando Padovani Azoubel,Reinaldo Di Mateo,Miguel Angel Sala Lopes,Ruberval Armando Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study |
| description |
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate karyometric and stereological alterations in the rat fetal pancreas resulting from the intraperitoneal administration of sodium cyclamate. The exocrine pancreas of ten fetuses of rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas; the length of the umbilical cords also were measured. After the laboratory processing, semi-seriated 6mm cuts stained with haematoxyline and eosine were performed. In seven karyometric parameters (major, minor, and medium diameters, volume, area, perimeter, and volume-area ratio), the increase was statistically significant in the treated group when compared with control group. Stereological parameters showed in the treated group a significant increase in the cellular volume and a significant reduction in the numerical cellular density. These results showed that the sodium cyclamate in pregnant rats led to retardation of fetal development and hypertrophy in the exocrine pancreas of the rat fetuses. |
| author |
Martins,Alex Tadeu Santos,Fabiano de Sant´Ana dos Scannavino,Fábio Luiz Ferreira Pires,Juliana Rico Zuza,Elizângela Partata Junior,João Armando Padovani Azoubel,Reinaldo Di Mateo,Miguel Angel Sala Lopes,Ruberval Armando |
| author_facet |
Martins,Alex Tadeu Santos,Fabiano de Sant´Ana dos Scannavino,Fábio Luiz Ferreira Pires,Juliana Rico Zuza,Elizângela Partata Junior,João Armando Padovani Azoubel,Reinaldo Di Mateo,Miguel Angel Sala Lopes,Ruberval Armando |
| author_sort |
Martins,Alex Tadeu |
| title |
Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study |
| title_short |
Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study |
| title_full |
Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study |
| title_fullStr |
Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study |
| title_full_unstemmed |
Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study |
| title_sort |
effect of sodium cyclamate on the rat fetal exocrine pancreas: a karyometric and stereological study |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2010 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300038 |
| work_keys_str_mv |
AT martinsalextadeu effectofsodiumcyclamateontheratfetalexocrinepancreasakaryometricandstereologicalstudy AT santosfabianodesantanados effectofsodiumcyclamateontheratfetalexocrinepancreasakaryometricandstereologicalstudy AT scannavinofabioluizferreira effectofsodiumcyclamateontheratfetalexocrinepancreasakaryometricandstereologicalstudy AT piresjulianarico effectofsodiumcyclamateontheratfetalexocrinepancreasakaryometricandstereologicalstudy AT zuzaelizangelapartata effectofsodiumcyclamateontheratfetalexocrinepancreasakaryometricandstereologicalstudy AT juniorjoaoarmandopadovani effectofsodiumcyclamateontheratfetalexocrinepancreasakaryometricandstereologicalstudy AT azoubelreinaldo effectofsodiumcyclamateontheratfetalexocrinepancreasakaryometricandstereologicalstudy AT dimateomiguelangelsala effectofsodiumcyclamateontheratfetalexocrinepancreasakaryometricandstereologicalstudy AT lopesrubervalarmando effectofsodiumcyclamateontheratfetalexocrinepancreasakaryometricandstereologicalstudy |
| _version_ |
1718444705481490432 |