Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats

In this study, we evaluated the ultrastructural findings of testis with systemic administration of different doses of melatonin during ischemic period in a rat model of testicular torsion/detorsion (T/D). Testis ischemia-reperfusion (I/R) injury was induced by torsion of the left testis, with a 720...

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Autores principales:	Aktas,Ayfer, Tuncer,M. Cudi, Yildirim,Ayse, Nergiz,Yusuf, Akkus,Murat
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Testis Torsion Detorsion Spraque-Dawley rat Melatonin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100001
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spelling	oai:scielo:S0717-950220110001000012011-09-21Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley RatsAktas,AyferTuncer,M. CudiYildirim,AyseNergiz,YusufAkkus,Murat Testis Torsion Detorsion Spraque-Dawley rat Melatonin In this study, we evaluated the ultrastructural findings of testis with systemic administration of different doses of melatonin during ischemic period in a rat model of testicular torsion/detorsion (T/D). Testis ischemia-reperfusion (I/R) injury was induced by torsion of the left testis, with a 720 degrees twisting of the spermatic cord so as to produce a total occlusion of testis for 2.5 hours. Subsequently, the same testis was then detorsioned. According to surgical procedure in each group, unilateral orchiectomies were performed for histopathologic examination. The groups were labelled as control group, torsion group (T), torsion and detorsion group (T/D), torsion-detorsion and melatonin group (T/D+20,50 and 100 mg/kg melatonin). For the histological examination, testicular tissues were fixed in 2.5% glutheraldehyde and postfixation 1% osmic acid solutions. They were examined under transmission electron microscopy after application of contrast stained. In torsion group testis cross-sections, cytoplasm residues of mature sperms and large vacuole-like structures were noticeable. In detorsion group testis cross-sections, dissociations in spermatocide nuclei, many vacuoles and residual particles resulting from organelle degeneration, local voids in cytoplasms of spermatogonia, dilatation in granulated endoplasmic reticulum, large lipid droplets, chromatid particles, along with mitochondrial crystalisis were determined. In the testis cross-sections of the group of T/D+50 mg/kg melatonin administration, sertoli and spermatogonia cells that showed membrane-like structures and cytoplasmic voids were observed. Testis cross-sections of rats that were administered with T/D+50 mg/kg melatonin showed small mitochondrions and vacuole-like structures placed on the edge. Testis cross-sections of rats that were administered with T/D+100 mg/kg melatonin resulted in views similar to those of controls in the microstructural level. As a result, the most effective dose of melatonin, which was used in different doses, for prevention of ischemia/reperfusion damage was found to be 100 mg/kg.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.1 20112011-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100001en10.4067/S0717-95022011000100001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Testis Torsion Detorsion Spraque-Dawley rat Melatonin
spellingShingle	Testis Torsion Detorsion Spraque-Dawley rat Melatonin Aktas,Ayfer Tuncer,M. Cudi Yildirim,Ayse Nergiz,Yusuf Akkus,Murat Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats
description	In this study, we evaluated the ultrastructural findings of testis with systemic administration of different doses of melatonin during ischemic period in a rat model of testicular torsion/detorsion (T/D). Testis ischemia-reperfusion (I/R) injury was induced by torsion of the left testis, with a 720 degrees twisting of the spermatic cord so as to produce a total occlusion of testis for 2.5 hours. Subsequently, the same testis was then detorsioned. According to surgical procedure in each group, unilateral orchiectomies were performed for histopathologic examination. The groups were labelled as control group, torsion group (T), torsion and detorsion group (T/D), torsion-detorsion and melatonin group (T/D+20,50 and 100 mg/kg melatonin). For the histological examination, testicular tissues were fixed in 2.5% glutheraldehyde and postfixation 1% osmic acid solutions. They were examined under transmission electron microscopy after application of contrast stained. In torsion group testis cross-sections, cytoplasm residues of mature sperms and large vacuole-like structures were noticeable. In detorsion group testis cross-sections, dissociations in spermatocide nuclei, many vacuoles and residual particles resulting from organelle degeneration, local voids in cytoplasms of spermatogonia, dilatation in granulated endoplasmic reticulum, large lipid droplets, chromatid particles, along with mitochondrial crystalisis were determined. In the testis cross-sections of the group of T/D+50 mg/kg melatonin administration, sertoli and spermatogonia cells that showed membrane-like structures and cytoplasmic voids were observed. Testis cross-sections of rats that were administered with T/D+50 mg/kg melatonin showed small mitochondrions and vacuole-like structures placed on the edge. Testis cross-sections of rats that were administered with T/D+100 mg/kg melatonin resulted in views similar to those of controls in the microstructural level. As a result, the most effective dose of melatonin, which was used in different doses, for prevention of ischemia/reperfusion damage was found to be 100 mg/kg.
author	Aktas,Ayfer Tuncer,M. Cudi Yildirim,Ayse Nergiz,Yusuf Akkus,Murat
author_facet	Aktas,Ayfer Tuncer,M. Cudi Yildirim,Ayse Nergiz,Yusuf Akkus,Murat
author_sort	Aktas,Ayfer
title	Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats
title_short	Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats
title_full	Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats
title_fullStr	Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats
title_full_unstemmed	Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats
title_sort	protective effects of melatonin on testicular torsion and detorsion damage in sprague-dawley rats
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100001
work_keys_str_mv	AT aktasayfer protectiveeffectsofmelatoninontesticulartorsionanddetorsiondamageinspraguedawleyrats AT tuncermcudi protectiveeffectsofmelatoninontesticulartorsionanddetorsiondamageinspraguedawleyrats AT yildirimayse protectiveeffectsofmelatoninontesticulartorsionanddetorsiondamageinspraguedawleyrats AT nergizyusuf protectiveeffectsofmelatoninontesticulartorsionanddetorsiondamageinspraguedawleyrats AT akkusmurat protectiveeffectsofmelatoninontesticulartorsionanddetorsiondamageinspraguedawleyrats
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Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats

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