Structure and Concentration of Elastic System Fibers in the Trachea of the Rat

The extracellular matrix is a key element in the tracheal morphology and physiology, both in normal and pathological states, yet little is known regarding its composition and organization. Herein we carried out a detailed study of the morphological organization and volumetric density of elastic syst...

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Autores principales:	Andrade,Filipe Moreira de, Judice,Luiz Felippe, Cisne,Rafael, Félix,Bruno, Mourad,Omar Moté Abou, Cardoso,Gilberto Perez, Babinski,Marcio Antonio
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Rat Trachea Morphology Elastic fibers Quantitative evaluation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100002
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spelling	oai:scielo:S0717-950220110001000022011-09-21Structure and Concentration of Elastic System Fibers in the Trachea of the RatAndrade,Filipe Moreira deJudice,Luiz FelippeCisne,RafaelFélix,BrunoMourad,Omar Moté AbouCardoso,Gilberto PerezBabinski,Marcio Antonio Rat Trachea Morphology Elastic fibers Quantitative evaluation The extracellular matrix is a key element in the tracheal morphology and physiology, both in normal and pathological states, yet little is known regarding its composition and organization. Herein we carried out a detailed study of the morphological organization and volumetric density of elastic system fibers in the trachea of Wistar rats. Six Wistar rats at the age of 45 days were used. The trachea was excised following anesthesia with Thiopental and perfusion through the left ventricle with buffered saline followed by formalin solution. Samples were fixed in formaline, embedded in paraffin, and histologically processed. The elastic system fibers were evaluated at light microscopy by using Weigert's resorcin-fuchsin technique after oxidation with oxone. Morphometric studies were performed by the point-counting method. Quantities were expressed (%, mean) as volumetric densities and were determined on 25 random fields for each animal. Elastic system fibers were identified beneath the tracheal mucosa showing mainly a longitudinal profile. There were also some bundles of oblique fibers in this region, forming an irregular network of elastic tissue. Close to the inner surface of the cartilaginous ring, a well defined circular layer is present. The volumetric density of the elastic system fibers in the trachea of Wistar rats is 2.46 ± 0.99%. The data in the present study provides original information regarding the elastic system fibers of the rat trachea, which might be used as a model for studying human major airway morphology. The results reported herein provide the basis for continuous investigations on tracheal extracellular matrix by stereology.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.1 20112011-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100002en10.4067/S0717-95022011000100002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Rat Trachea Morphology Elastic fibers Quantitative evaluation
spellingShingle	Rat Trachea Morphology Elastic fibers Quantitative evaluation Andrade,Filipe Moreira de Judice,Luiz Felippe Cisne,Rafael Félix,Bruno Mourad,Omar Moté Abou Cardoso,Gilberto Perez Babinski,Marcio Antonio Structure and Concentration of Elastic System Fibers in the Trachea of the Rat
description	The extracellular matrix is a key element in the tracheal morphology and physiology, both in normal and pathological states, yet little is known regarding its composition and organization. Herein we carried out a detailed study of the morphological organization and volumetric density of elastic system fibers in the trachea of Wistar rats. Six Wistar rats at the age of 45 days were used. The trachea was excised following anesthesia with Thiopental and perfusion through the left ventricle with buffered saline followed by formalin solution. Samples were fixed in formaline, embedded in paraffin, and histologically processed. The elastic system fibers were evaluated at light microscopy by using Weigert's resorcin-fuchsin technique after oxidation with oxone. Morphometric studies were performed by the point-counting method. Quantities were expressed (%, mean) as volumetric densities and were determined on 25 random fields for each animal. Elastic system fibers were identified beneath the tracheal mucosa showing mainly a longitudinal profile. There were also some bundles of oblique fibers in this region, forming an irregular network of elastic tissue. Close to the inner surface of the cartilaginous ring, a well defined circular layer is present. The volumetric density of the elastic system fibers in the trachea of Wistar rats is 2.46 ± 0.99%. The data in the present study provides original information regarding the elastic system fibers of the rat trachea, which might be used as a model for studying human major airway morphology. The results reported herein provide the basis for continuous investigations on tracheal extracellular matrix by stereology.
author	Andrade,Filipe Moreira de Judice,Luiz Felippe Cisne,Rafael Félix,Bruno Mourad,Omar Moté Abou Cardoso,Gilberto Perez Babinski,Marcio Antonio
author_facet	Andrade,Filipe Moreira de Judice,Luiz Felippe Cisne,Rafael Félix,Bruno Mourad,Omar Moté Abou Cardoso,Gilberto Perez Babinski,Marcio Antonio
author_sort	Andrade,Filipe Moreira de
title	Structure and Concentration of Elastic System Fibers in the Trachea of the Rat
title_short	Structure and Concentration of Elastic System Fibers in the Trachea of the Rat
title_full	Structure and Concentration of Elastic System Fibers in the Trachea of the Rat
title_fullStr	Structure and Concentration of Elastic System Fibers in the Trachea of the Rat
title_full_unstemmed	Structure and Concentration of Elastic System Fibers in the Trachea of the Rat
title_sort	structure and concentration of elastic system fibers in the trachea of the rat
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100002
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Structure and Concentration of Elastic System Fibers in the Trachea of the Rat

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