Histochemical Study of Early Embryo Implantation in Rats

Embryo implantation is the process that results in attachment of the conceptus to the uterine wall. In this histochemical study, we investigated the early stage of embryo implantation in rats by morphological analysis and by the detection of total proteins and glycosaminoglycans using hematoxylin-eo...

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Autores principales:	Spósito,Danilo Rodrigues, Santos Jr,Arnaldo Rodrigues
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Implantation window Endometrium Trophoblast Embryo
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100032
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spelling	oai:scielo:S0717-950220110001000322011-09-21Histochemical Study of Early Embryo Implantation in RatsSpósito,Danilo RodriguesSantos Jr,Arnaldo Rodrigues Implantation window Endometrium Trophoblast Embryo Embryo implantation is the process that results in attachment of the conceptus to the uterine wall. In this histochemical study, we investigated the early stage of embryo implantation in rats by morphological analysis and by the detection of total proteins and glycosaminoglycans using hematoxylin-eosin, toluidine blue at pH 4.0 (TB), and Xylidine Ponceau at pH 2.5 (XP). In non-pregnant females, the uterine layers could be clearly distinguished and showed the normal histology of the organ. In pregnant females, an increase in the number of cells and a reduction in the interstitial space were observed in the endometrium close to the implantation sites. The blastocyst was partially inserted in the endometrium, with the observation of the inner cells mass around the blastocyst cavity surrounded by trophoblastic cells. TB staining revealed mild metachromatic basophilia, which was more evident in the endometrial stroma around the implantation site. Histochemical staining with XP was also more intense in the stroma close to the site of implantation. On the other hand, histochemical staining with either TB or XP was more discrete at sites distant from the conceptus. This study demonstrated changes in the endometrial stroma in areas adjacent to the site of embryo implantation, with variations in glycosaminoglycans and proteins as demonstrated by the detection of anionic and cationic radicals, respectively.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.1 20112011-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100032en10.4067/S0717-95022011000100032
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Implantation window Endometrium Trophoblast Embryo
spellingShingle	Implantation window Endometrium Trophoblast Embryo Spósito,Danilo Rodrigues Santos Jr,Arnaldo Rodrigues Histochemical Study of Early Embryo Implantation in Rats
description	Embryo implantation is the process that results in attachment of the conceptus to the uterine wall. In this histochemical study, we investigated the early stage of embryo implantation in rats by morphological analysis and by the detection of total proteins and glycosaminoglycans using hematoxylin-eosin, toluidine blue at pH 4.0 (TB), and Xylidine Ponceau at pH 2.5 (XP). In non-pregnant females, the uterine layers could be clearly distinguished and showed the normal histology of the organ. In pregnant females, an increase in the number of cells and a reduction in the interstitial space were observed in the endometrium close to the implantation sites. The blastocyst was partially inserted in the endometrium, with the observation of the inner cells mass around the blastocyst cavity surrounded by trophoblastic cells. TB staining revealed mild metachromatic basophilia, which was more evident in the endometrial stroma around the implantation site. Histochemical staining with XP was also more intense in the stroma close to the site of implantation. On the other hand, histochemical staining with either TB or XP was more discrete at sites distant from the conceptus. This study demonstrated changes in the endometrial stroma in areas adjacent to the site of embryo implantation, with variations in glycosaminoglycans and proteins as demonstrated by the detection of anionic and cationic radicals, respectively.
author	Spósito,Danilo Rodrigues Santos Jr,Arnaldo Rodrigues
author_facet	Spósito,Danilo Rodrigues Santos Jr,Arnaldo Rodrigues
author_sort	Spósito,Danilo Rodrigues
title	Histochemical Study of Early Embryo Implantation in Rats
title_short	Histochemical Study of Early Embryo Implantation in Rats
title_full	Histochemical Study of Early Embryo Implantation in Rats
title_fullStr	Histochemical Study of Early Embryo Implantation in Rats
title_full_unstemmed	Histochemical Study of Early Embryo Implantation in Rats
title_sort	histochemical study of early embryo implantation in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100032
work_keys_str_mv	AT spositodanilorodrigues histochemicalstudyofearlyembryoimplantationinrats AT santosjrarnaldorodrigues histochemicalstudyofearlyembryoimplantationinrats
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Histochemical Study of Early Embryo Implantation in Rats

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