Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic Rats

It is widely described in the literature that diabetic patients present hearing impairment. Despite the histological alterations of the internal ear structures in these patients as well as in experimental models of diabetes, to the best of our knowledge, an histological evaluation of the vestibuloco...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vasconcelos,Carlos Augusto Carvalho de, Fazan,Valéria Paula Sassoli, Moore,Kenneth Charles, Nessler,Randy Alan, Valença,Marcelo Moraes
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Experimental diabetes Vestibulocochlear nerve Ultrastructure Myelinated fibers Axonal atrophy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100045
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022011000100045
record_format	dspace
spelling	oai:scielo:S0717-950220110001000452011-09-21Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic RatsVasconcelos,Carlos Augusto Carvalho deFazan,Valéria Paula SassoliMoore,Kenneth CharlesNessler,Randy AlanValença,Marcelo Moraes Experimental diabetes Vestibulocochlear nerve Ultrastructure Myelinated fibers Axonal atrophy It is widely described in the literature that diabetic patients present hearing impairment. Despite the histological alterations of the internal ear structures in these patients as well as in experimental models of diabetes, to the best of our knowledge, an histological evaluation of the vestibulocochlear nerve have not been performed. In the present study, ultrastructural alterations are described and compared between a spinal nerves and a cranial nerve in rats with chronic induced diabetes. Male Wistar rats (n = 12), fed with standard diet from the animal care facility at 42 days of age were used. Induced diabetic animals (n=6) were fasted for 12 hours prior to being injected intraperitoneally with streptozotocin (STZ - 60mg/kg) in a single dose. Control animals (n=6) received (0.01 mol/l citrate buffer, pH 4.5) vehicle alone. Ten weeks after STZ injection the animals were perfused intracardially with Karnovsky solution. Right and left vestibulocochlear nerves were dissected and histologically processed for epoxy resin embedding. Samples were imaged with the transmission electron microscope. Large myelinated fibers with morphological signs of axonal atrophy in the vestibulocochlear nerves were readily observed. These results suggest that chronic STZ-induced diabetes in rats caused alterations in the myelinated fibers and Schwann cells, compatible to the classic diabetes signs and symptoms. Morphological alterations of the vestibulocochlear nerve in diabetes is described for the first time and contributes information for a better understanding of why there are changes in hearing observed in diabetic patients.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.1 20112011-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100045en10.4067/S0717-95022011000100045
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Experimental diabetes Vestibulocochlear nerve Ultrastructure Myelinated fibers Axonal atrophy
spellingShingle	Experimental diabetes Vestibulocochlear nerve Ultrastructure Myelinated fibers Axonal atrophy Vasconcelos,Carlos Augusto Carvalho de Fazan,Valéria Paula Sassoli Moore,Kenneth Charles Nessler,Randy Alan Valença,Marcelo Moraes Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic Rats
description	It is widely described in the literature that diabetic patients present hearing impairment. Despite the histological alterations of the internal ear structures in these patients as well as in experimental models of diabetes, to the best of our knowledge, an histological evaluation of the vestibulocochlear nerve have not been performed. In the present study, ultrastructural alterations are described and compared between a spinal nerves and a cranial nerve in rats with chronic induced diabetes. Male Wistar rats (n = 12), fed with standard diet from the animal care facility at 42 days of age were used. Induced diabetic animals (n=6) were fasted for 12 hours prior to being injected intraperitoneally with streptozotocin (STZ - 60mg/kg) in a single dose. Control animals (n=6) received (0.01 mol/l citrate buffer, pH 4.5) vehicle alone. Ten weeks after STZ injection the animals were perfused intracardially with Karnovsky solution. Right and left vestibulocochlear nerves were dissected and histologically processed for epoxy resin embedding. Samples were imaged with the transmission electron microscope. Large myelinated fibers with morphological signs of axonal atrophy in the vestibulocochlear nerves were readily observed. These results suggest that chronic STZ-induced diabetes in rats caused alterations in the myelinated fibers and Schwann cells, compatible to the classic diabetes signs and symptoms. Morphological alterations of the vestibulocochlear nerve in diabetes is described for the first time and contributes information for a better understanding of why there are changes in hearing observed in diabetic patients.
author	Vasconcelos,Carlos Augusto Carvalho de Fazan,Valéria Paula Sassoli Moore,Kenneth Charles Nessler,Randy Alan Valença,Marcelo Moraes
author_facet	Vasconcelos,Carlos Augusto Carvalho de Fazan,Valéria Paula Sassoli Moore,Kenneth Charles Nessler,Randy Alan Valença,Marcelo Moraes
author_sort	Vasconcelos,Carlos Augusto Carvalho de
title	Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic Rats
title_short	Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic Rats
title_full	Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic Rats
title_fullStr	Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic Rats
title_full_unstemmed	Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic Rats
title_sort	transmission electron microscopy studies of the vestibulocochlear nerve in chronic diabetic rats
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100045
work_keys_str_mv	AT vasconceloscarlosaugustocarvalhode transmissionelectronmicroscopystudiesofthevestibulocochlearnerveinchronicdiabeticrats AT fazanvaleriapaulasassoli transmissionelectronmicroscopystudiesofthevestibulocochlearnerveinchronicdiabeticrats AT moorekennethcharles transmissionelectronmicroscopystudiesofthevestibulocochlearnerveinchronicdiabeticrats AT nesslerrandyalan transmissionelectronmicroscopystudiesofthevestibulocochlearnerveinchronicdiabeticrats AT valencamarcelomoraes transmissionelectronmicroscopystudiesofthevestibulocochlearnerveinchronicdiabeticrats
_version_	1718444725598420992

Transmission Electron Microscopy Studies of the Vestibulocochlear Nerve in Chronic Diabetic Rats

Ejemplares similares