Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles

The administration of microencapsulated drug in a matrix acid poly(lactic-co-glycolic acid) (PLGA) by intramuscular (IM) in humans has been approved by the FDA for various applications though it is not clear what effect they have on the morphological parameters of muscle tissue. The aim of this stud...

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Autores principales:	Acuña,Luis, Suazo Galdames,Iván, Zavando,Daniela, Elgueta,Samanta, Velásquez,Luis, Vilos,Cristian, Cantín,Mario
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Skeletal muscle Drug delivery Polymeric microparticles PLGA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200016
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spelling	oai:scielo:S0717-950220110002000162011-07-22Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA MicroparticlesAcuña,LuisSuazo Galdames,IvánZavando,DanielaElgueta,SamantaVelásquez,LuisVilos,CristianCantín,Mario Skeletal muscle Drug delivery Polymeric microparticles PLGA The administration of microencapsulated drug in a matrix acid poly(lactic-co-glycolic acid) (PLGA) by intramuscular (IM) in humans has been approved by the FDA for various applications though it is not clear what effect they have on the morphological parameters of muscle tissue. The aim of this study was to analyze the morphological changes in the skeletal muscle tissue with their use. We used 12 adult female Sprague Dawley rats (Rattus novergicus) that were injected into their right gastrocnemius muscle belly with: sterile vehicle solution (G1, n = 4), 0.5 mg PLGA microparticle (G2, n = 4) and 0.75 mg PLGA microparticle (G3, n = 4), both dissolved in a sterile vehicle solution. At 14 days post injection the number and diameter of muscle fibers, the level of inflammation and histology appearance in terms of organization of muscle fibers, cellular distribution, tissue morphology and the presence of polymer waste were determined and the results between the groups compared. The administration of the compound in a single dose did not alter the morphometric parameters (number and diameter of muscle fibers) despite generating a mild inflammation in the tissue associated with the presence of polymeric residues, suggesting that the PLGA microparticles were well tolerated by the muscle tissue at concentrations tested (0.5 and 0.75 mg).info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.2 20112011-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200016en10.4067/S0717-95022011000200016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Skeletal muscle Drug delivery Polymeric microparticles PLGA
spellingShingle	Skeletal muscle Drug delivery Polymeric microparticles PLGA Acuña,Luis Suazo Galdames,Iván Zavando,Daniela Elgueta,Samanta Velásquez,Luis Vilos,Cristian Cantín,Mario Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles
description	The administration of microencapsulated drug in a matrix acid poly(lactic-co-glycolic acid) (PLGA) by intramuscular (IM) in humans has been approved by the FDA for various applications though it is not clear what effect they have on the morphological parameters of muscle tissue. The aim of this study was to analyze the morphological changes in the skeletal muscle tissue with their use. We used 12 adult female Sprague Dawley rats (Rattus novergicus) that were injected into their right gastrocnemius muscle belly with: sterile vehicle solution (G1, n = 4), 0.5 mg PLGA microparticle (G2, n = 4) and 0.75 mg PLGA microparticle (G3, n = 4), both dissolved in a sterile vehicle solution. At 14 days post injection the number and diameter of muscle fibers, the level of inflammation and histology appearance in terms of organization of muscle fibers, cellular distribution, tissue morphology and the presence of polymer waste were determined and the results between the groups compared. The administration of the compound in a single dose did not alter the morphometric parameters (number and diameter of muscle fibers) despite generating a mild inflammation in the tissue associated with the presence of polymeric residues, suggesting that the PLGA microparticles were well tolerated by the muscle tissue at concentrations tested (0.5 and 0.75 mg).
author	Acuña,Luis Suazo Galdames,Iván Zavando,Daniela Elgueta,Samanta Velásquez,Luis Vilos,Cristian Cantín,Mario
author_facet	Acuña,Luis Suazo Galdames,Iván Zavando,Daniela Elgueta,Samanta Velásquez,Luis Vilos,Cristian Cantín,Mario
author_sort	Acuña,Luis
title	Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles
title_short	Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles
title_full	Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles
title_fullStr	Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles
title_full_unstemmed	Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles
title_sort	morphometric and histopathologic changes in skeletal muscle induced for injectable plga microparticles
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200016
work_keys_str_mv	AT acunaluis morphometricandhistopathologicchangesinskeletalmuscleinducedforinjectableplgamicroparticles AT suazogaldamesivan morphometricandhistopathologicchangesinskeletalmuscleinducedforinjectableplgamicroparticles AT zavandodaniela morphometricandhistopathologicchangesinskeletalmuscleinducedforinjectableplgamicroparticles AT elguetasamanta morphometricandhistopathologicchangesinskeletalmuscleinducedforinjectableplgamicroparticles AT velasquezluis morphometricandhistopathologicchangesinskeletalmuscleinducedforinjectableplgamicroparticles AT viloscristian morphometricandhistopathologicchangesinskeletalmuscleinducedforinjectableplgamicroparticles AT cantinmario morphometricandhistopathologicchangesinskeletalmuscleinducedforinjectableplgamicroparticles
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Morphometric and Histopathologic Changes in Skeletal Muscle Induced for Injectable PLGA Microparticles

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