Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy

The pigeon feces are vehicle of diseases both for humans and other animal species. In these birds, the most important viral diseases of the digestive tract are transmitted by the paramyxovirus, adenovirus and coronavirus. Avian paramyxoviruses have been isolated from a variety of species of free liv...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Catroxo,M. H. B, Martins,A. M. C. R. P. F, Petrella,S, Curi,N. A, Melo,N.A
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Paramyxovirus Adenovirus Coronavirus Pigeons Transmission electron microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200055
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022011000200055
record_format	dspace
spelling	oai:scielo:S0717-950220110002000552011-07-22Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron MicroscopyCatroxo,M. H. BMartins,A. M. C. R. P. FPetrella,SCuri,N. AMelo,N.A Paramyxovirus Adenovirus Coronavirus Pigeons Transmission electron microscopy The pigeon feces are vehicle of diseases both for humans and other animal species. In these birds, the most important viral diseases of the digestive tract are transmitted by the paramyxovirus, adenovirus and coronavirus. Avian paramyxoviruses have been isolated from a variety of species of free living and domestic birds worldwide, with several symptoms and clinical signs and economic losses. Paramyxoviruses belong to the Paramyxoviridae family and Avulovirus genus that includes nine serotypes (APMV 1 to 9). Avian adenoviruses belong to the Adenoviridae family and Aviadenovirus genus. In pigeons, cause classical adenovirosis and necrotizing hepatitis. The respiratory and enteric tracts are common targets of coronavirus. They belong to the Coronaviridae family and to 3a and 3c groups. In this study, we described the presence of viral agents in free-living pigeon feces (Columba livia) from the city of São Paulo, Brazil. The feces were processed by negative staining technique (rapid preparation) for transmission electron microscopy. In this technique paramyxoviruses particles, pleomorphic, roughly spherical or filamentous, measuring 100 to 500 nm of diameter containing an envelope covered by spikes, with characteristic helical herring-bone-like nucleocapsid, measuring 15 to 20 nm in diameter, were visualized in 45 (79%) out of 57 feces samples. In 2 (3.5%) samples, paramyxovirus and adenovirus particles were simultaneously visualized. Adenovirus particles were isometric, spherical, characterized as "complete "or" empty ", measuring between 70 and 90 nm in diameter. Paramyxovirus and coronavirus particles were detected in 3 (5.2%) samples. Coronaviruses were pleomorphic with a diameter of 75-160 nm containing a solar corona-shaped envelope, with projections of approximately 20 nm of diameter. Seven (12.3%) samples were negative for viral particles.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.2 20112011-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200055en10.4067/S0717-95022011000200055
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Paramyxovirus Adenovirus Coronavirus Pigeons Transmission electron microscopy
spellingShingle	Paramyxovirus Adenovirus Coronavirus Pigeons Transmission electron microscopy Catroxo,M. H. B Martins,A. M. C. R. P. F Petrella,S Curi,N. A Melo,N.A Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy
description	The pigeon feces are vehicle of diseases both for humans and other animal species. In these birds, the most important viral diseases of the digestive tract are transmitted by the paramyxovirus, adenovirus and coronavirus. Avian paramyxoviruses have been isolated from a variety of species of free living and domestic birds worldwide, with several symptoms and clinical signs and economic losses. Paramyxoviruses belong to the Paramyxoviridae family and Avulovirus genus that includes nine serotypes (APMV 1 to 9). Avian adenoviruses belong to the Adenoviridae family and Aviadenovirus genus. In pigeons, cause classical adenovirosis and necrotizing hepatitis. The respiratory and enteric tracts are common targets of coronavirus. They belong to the Coronaviridae family and to 3a and 3c groups. In this study, we described the presence of viral agents in free-living pigeon feces (Columba livia) from the city of São Paulo, Brazil. The feces were processed by negative staining technique (rapid preparation) for transmission electron microscopy. In this technique paramyxoviruses particles, pleomorphic, roughly spherical or filamentous, measuring 100 to 500 nm of diameter containing an envelope covered by spikes, with characteristic helical herring-bone-like nucleocapsid, measuring 15 to 20 nm in diameter, were visualized in 45 (79%) out of 57 feces samples. In 2 (3.5%) samples, paramyxovirus and adenovirus particles were simultaneously visualized. Adenovirus particles were isometric, spherical, characterized as "complete "or" empty ", measuring between 70 and 90 nm in diameter. Paramyxovirus and coronavirus particles were detected in 3 (5.2%) samples. Coronaviruses were pleomorphic with a diameter of 75-160 nm containing a solar corona-shaped envelope, with projections of approximately 20 nm of diameter. Seven (12.3%) samples were negative for viral particles.
author	Catroxo,M. H. B Martins,A. M. C. R. P. F Petrella,S Curi,N. A Melo,N.A
author_facet	Catroxo,M. H. B Martins,A. M. C. R. P. F Petrella,S Curi,N. A Melo,N.A
author_sort	Catroxo,M. H. B
title	Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy
title_short	Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy
title_full	Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy
title_fullStr	Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy
title_full_unstemmed	Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy
title_sort	research of viral agent in free-living pigeon feces (columba livia) in the city of são paulo, sp, brazil, for transmission electron microscopy
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200055
work_keys_str_mv	AT catroxomhb researchofviralagentinfreelivingpigeonfecescolumbaliviainthecityofsaopaulospbrazilfortransmissionelectronmicroscopy AT martinsamcrpf researchofviralagentinfreelivingpigeonfecescolumbaliviainthecityofsaopaulospbrazilfortransmissionelectronmicroscopy AT petrellas researchofviralagentinfreelivingpigeonfecescolumbaliviainthecityofsaopaulospbrazilfortransmissionelectronmicroscopy AT curina researchofviralagentinfreelivingpigeonfecescolumbaliviainthecityofsaopaulospbrazilfortransmissionelectronmicroscopy AT melona researchofviralagentinfreelivingpigeonfecescolumbaliviainthecityofsaopaulospbrazilfortransmissionelectronmicroscopy
_version_	1718444736134512640

Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy

Ejemplares similares