Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study

Glutaraldehyde is the fixative most commonly used in electron microscope studies of biological tissues, however it is often necessary to use samples which were not fixed in this fixative, even with the usual uncertainty of the results that may be obtained. The fixation is the more delicate step of t...

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Autores principales:	Guerrero,F, Cerutti,P, Marcaccini,A
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Scanning electron microscopy Fixation Fixative Haemal node Glutaraldehyde Formaldehyde
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200059
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spelling	oai:scielo:S0717-950220110002000592011-07-22Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic StudyGuerrero,FCerutti,PMarcaccini,A Scanning electron microscopy Fixation Fixative Haemal node Glutaraldehyde Formaldehyde Glutaraldehyde is the fixative most commonly used in electron microscope studies of biological tissues, however it is often necessary to use samples which were not fixed in this fixative, even with the usual uncertainty of the results that may be obtained. The fixation is the more delicate step of the sample processing. Therefore in this work, the quality of preservation of haemal nodes fixed with two classic aldehyde fixatives: formaldehyde and glutaraldehyde we have compared under the scanning electron microscope. Our results showed that both fixatives were successful in preserving the morphology of haemal nodes components; however glutaraldehyde conferred satisfactory results mainly in the preservation of parenchymal cells, whereas formaldehyde was better for preservation of stromal fibres.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.2 20112011-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200059en10.4067/S0717-95022011000200059
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Scanning electron microscopy Fixation Fixative Haemal node Glutaraldehyde Formaldehyde
spellingShingle	Scanning electron microscopy Fixation Fixative Haemal node Glutaraldehyde Formaldehyde Guerrero,F Cerutti,P Marcaccini,A Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study
description	Glutaraldehyde is the fixative most commonly used in electron microscope studies of biological tissues, however it is often necessary to use samples which were not fixed in this fixative, even with the usual uncertainty of the results that may be obtained. The fixation is the more delicate step of the sample processing. Therefore in this work, the quality of preservation of haemal nodes fixed with two classic aldehyde fixatives: formaldehyde and glutaraldehyde we have compared under the scanning electron microscope. Our results showed that both fixatives were successful in preserving the morphology of haemal nodes components; however glutaraldehyde conferred satisfactory results mainly in the preservation of parenchymal cells, whereas formaldehyde was better for preservation of stromal fibres.
author	Guerrero,F Cerutti,P Marcaccini,A
author_facet	Guerrero,F Cerutti,P Marcaccini,A
author_sort	Guerrero,F
title	Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study
title_short	Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study
title_full	Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study
title_fullStr	Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study
title_full_unstemmed	Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study
title_sort	preservation of haemal nodes fixed by means of two different aldehyde fixatives: a scanning electron microscopic study
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200059
work_keys_str_mv	AT guerrerof preservationofhaemalnodesfixedbymeansoftwodifferentaldehydefixativesascanningelectronmicroscopicstudy AT ceruttip preservationofhaemalnodesfixedbymeansoftwodifferentaldehydefixativesascanningelectronmicroscopicstudy AT marcaccinia preservationofhaemalnodesfixedbymeansoftwodifferentaldehydefixativesascanningelectronmicroscopicstudy
_version_	1718444736847544320

Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study

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