Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development

The aim of this work was to evaluate the effect of albendazole sulphoxide (ABZSO) administered to Balb C mice prior to mating on fertilization rate and preimplantational embryo development. Twenty four female mice 5-8 weeks of age were superovulated by intraperitoneal injection of 7.5 UI of equine c...

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Autores principales:	Teruel,M, Catalano,R, Salomón,L
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Albendazole Sulphoxide Reproductive toxicity Embryo development Mouse
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300024
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spelling	oai:scielo:S0717-950220110003000242012-06-11Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo DevelopmentTeruel,MCatalano,RSalomón,L Albendazole Sulphoxide Reproductive toxicity Embryo development Mouse The aim of this work was to evaluate the effect of albendazole sulphoxide (ABZSO) administered to Balb C mice prior to mating on fertilization rate and preimplantational embryo development. Twenty four female mice 5-8 weeks of age were superovulated by intraperitoneal injection of 7.5 UI of equine chorionic gonadotropin (eCG, Novormon®, Laboratorios Syntex S.A., Argentina); 48 h later they received 10 IU of human chorionic gonadotropin (hCG, Profasi®, Laboratorios Serono, Méjico) and were paired with males of proven fertility. Females received 100 mg/kg or 200 mg/kg of ABZSO orally at the time of hCG administration, prior to mating. The control group received carboxymethylcellulose, vehicle used to prepare the drug suspension. Pregnant females were killed by cervical dislocation at day 4 of pregnancy and non fertilized oocyte and embryos were flushed from uteri. The possible effects of ABZSO were evaluated considering the fertilization rate, the total number of collected embryos per female; the percentage of embryos morphologically normal; the differentiation rate (determined by the relation between the number of blastocyst and the total of morphologically normal embryos) and the cleavage rate determined by counting the nuclei. The variables were analyzed on a per litter basis using the Kruskal-Wallis test. The fertilization rate was lower in females administered ABZSO at a dose of 200 mg/kg (P<0.05). However, no statistically significant differences were found in the embryonic parameters after the administration of 100 mg/kg or 200 mg/kg of ABZSO compared to the untreated control group (P>0.05). In conclusion, a single acute exposure to ABZSO prior to mating at around the time of fertilization at a dose higher than the one usually administered in human and veterinary medicine affects the fertilization rate but it has no adverse effects on early embryo development.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.3 20112011-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300024en10.4067/S0717-95022011000300024
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Albendazole Sulphoxide Reproductive toxicity Embryo development Mouse
spellingShingle	Albendazole Sulphoxide Reproductive toxicity Embryo development Mouse Teruel,M Catalano,R Salomón,L Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development
description	The aim of this work was to evaluate the effect of albendazole sulphoxide (ABZSO) administered to Balb C mice prior to mating on fertilization rate and preimplantational embryo development. Twenty four female mice 5-8 weeks of age were superovulated by intraperitoneal injection of 7.5 UI of equine chorionic gonadotropin (eCG, Novormon®, Laboratorios Syntex S.A., Argentina); 48 h later they received 10 IU of human chorionic gonadotropin (hCG, Profasi®, Laboratorios Serono, Méjico) and were paired with males of proven fertility. Females received 100 mg/kg or 200 mg/kg of ABZSO orally at the time of hCG administration, prior to mating. The control group received carboxymethylcellulose, vehicle used to prepare the drug suspension. Pregnant females were killed by cervical dislocation at day 4 of pregnancy and non fertilized oocyte and embryos were flushed from uteri. The possible effects of ABZSO were evaluated considering the fertilization rate, the total number of collected embryos per female; the percentage of embryos morphologically normal; the differentiation rate (determined by the relation between the number of blastocyst and the total of morphologically normal embryos) and the cleavage rate determined by counting the nuclei. The variables were analyzed on a per litter basis using the Kruskal-Wallis test. The fertilization rate was lower in females administered ABZSO at a dose of 200 mg/kg (P<0.05). However, no statistically significant differences were found in the embryonic parameters after the administration of 100 mg/kg or 200 mg/kg of ABZSO compared to the untreated control group (P>0.05). In conclusion, a single acute exposure to ABZSO prior to mating at around the time of fertilization at a dose higher than the one usually administered in human and veterinary medicine affects the fertilization rate but it has no adverse effects on early embryo development.
author	Teruel,M Catalano,R Salomón,L
author_facet	Teruel,M Catalano,R Salomón,L
author_sort	Teruel,M
title	Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development
title_short	Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development
title_full	Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development
title_fullStr	Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development
title_full_unstemmed	Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development
title_sort	albendazole sulphoxide administered prior to mating andits relation with fertilization and mouse embryo development
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300024
work_keys_str_mv	AT teruelm albendazolesulphoxideadministeredpriortomatinganditsrelationwithfertilizationandmouseembryodevelopment AT catalanor albendazolesulphoxideadministeredpriortomatinganditsrelationwithfertilizationandmouseembryodevelopment AT salomonl albendazolesulphoxideadministeredpriortomatinganditsrelationwithfertilizationandmouseembryodevelopment
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Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development

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