Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)

The aim of the study was to analyze the muscle fibers by histochemistry and morphometric methods from patients with Duchenne muscular dystrophy (DMD). Muscle biopsies were taken from the vastus lateralis muscle of five boys between 13 and 15-years of age, with clinical diagnosis of DMD. The histoche...

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Autores principales: Cavalcanti,George Maciel, Oliveira,André de Sá Braga, Assis,Thiago de Oliveira, Chimelli,Leila Maria Cardão, Medeiros,Paloma Lys de, Mota,Diógenes Luís da
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2011
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300046
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spelling oai:scielo:S0717-950220110003000462012-06-11Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)Cavalcanti,George MacielOliveira,André de Sá BragaAssis,Thiago de OliveiraChimelli,Leila Maria CardãoMedeiros,Paloma Lys deMota,Diógenes Luís da Histochemistry analysis Morphometric analysis Vastus lateralis muscle Duchenne muscular dystrophy The aim of the study was to analyze the muscle fibers by histochemistry and morphometric methods from patients with Duchenne muscular dystrophy (DMD). Muscle biopsies were taken from the vastus lateralis muscle of five boys between 13 and 15-years of age, with clinical diagnosis of DMD. The histochemistry was performed using myofibrillar ATPases (9.6, 4.6 and 4.3). To morphometrical analysis a computerized semiautomatic system and software Image-Lab was used. ATPase staining showed atrophy of muscle fibers. Fibrosis and adipose deposition occurred in variable degree depending of muscular involvement. The morphometrical analysis showed an increase of size (percentage) to type I fiber than other types in all patients. Furthermore, the type I fiber had a larger cross-sectional area and mean diameter than type IIa and IIb fibers. Both histochemistry and morphometric analysis could be important tools for qualitative and quantitative diagnostics of muscle fibers attacked in this type of disease.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.3 20112011-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300046en10.4067/S0717-95022011000300046
institution Scielo Chile
collection Scielo Chile
language English
topic Histochemistry analysis
Morphometric analysis
Vastus lateralis muscle
Duchenne muscular dystrophy
spellingShingle Histochemistry analysis
Morphometric analysis
Vastus lateralis muscle
Duchenne muscular dystrophy
Cavalcanti,George Maciel
Oliveira,André de Sá Braga
Assis,Thiago de Oliveira
Chimelli,Leila Maria Cardão
Medeiros,Paloma Lys de
Mota,Diógenes Luís da
Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)
description The aim of the study was to analyze the muscle fibers by histochemistry and morphometric methods from patients with Duchenne muscular dystrophy (DMD). Muscle biopsies were taken from the vastus lateralis muscle of five boys between 13 and 15-years of age, with clinical diagnosis of DMD. The histochemistry was performed using myofibrillar ATPases (9.6, 4.6 and 4.3). To morphometrical analysis a computerized semiautomatic system and software Image-Lab was used. ATPase staining showed atrophy of muscle fibers. Fibrosis and adipose deposition occurred in variable degree depending of muscular involvement. The morphometrical analysis showed an increase of size (percentage) to type I fiber than other types in all patients. Furthermore, the type I fiber had a larger cross-sectional area and mean diameter than type IIa and IIb fibers. Both histochemistry and morphometric analysis could be important tools for qualitative and quantitative diagnostics of muscle fibers attacked in this type of disease.
author Cavalcanti,George Maciel
Oliveira,André de Sá Braga
Assis,Thiago de Oliveira
Chimelli,Leila Maria Cardão
Medeiros,Paloma Lys de
Mota,Diógenes Luís da
author_facet Cavalcanti,George Maciel
Oliveira,André de Sá Braga
Assis,Thiago de Oliveira
Chimelli,Leila Maria Cardão
Medeiros,Paloma Lys de
Mota,Diógenes Luís da
author_sort Cavalcanti,George Maciel
title Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)
title_short Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)
title_full Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)
title_fullStr Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)
title_full_unstemmed Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)
title_sort histochemistry and morphometric analysis of muscle fibers from patients with duchenne muscular dystrophy (dmd)
publisher Sociedad Chilena de Anatomía
publishDate 2011
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300046
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