Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)
The aim of the study was to analyze the muscle fibers by histochemistry and morphometric methods from patients with Duchenne muscular dystrophy (DMD). Muscle biopsies were taken from the vastus lateralis muscle of five boys between 13 and 15-years of age, with clinical diagnosis of DMD. The histoche...
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Sociedad Chilena de Anatomía
2011
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oai:scielo:S0717-950220110003000462012-06-11Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD)Cavalcanti,George MacielOliveira,André de Sá BragaAssis,Thiago de OliveiraChimelli,Leila Maria CardãoMedeiros,Paloma Lys deMota,Diógenes Luís da Histochemistry analysis Morphometric analysis Vastus lateralis muscle Duchenne muscular dystrophy The aim of the study was to analyze the muscle fibers by histochemistry and morphometric methods from patients with Duchenne muscular dystrophy (DMD). Muscle biopsies were taken from the vastus lateralis muscle of five boys between 13 and 15-years of age, with clinical diagnosis of DMD. The histochemistry was performed using myofibrillar ATPases (9.6, 4.6 and 4.3). To morphometrical analysis a computerized semiautomatic system and software Image-Lab was used. ATPase staining showed atrophy of muscle fibers. Fibrosis and adipose deposition occurred in variable degree depending of muscular involvement. The morphometrical analysis showed an increase of size (percentage) to type I fiber than other types in all patients. Furthermore, the type I fiber had a larger cross-sectional area and mean diameter than type IIa and IIb fibers. Both histochemistry and morphometric analysis could be important tools for qualitative and quantitative diagnostics of muscle fibers attacked in this type of disease.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.3 20112011-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300046en10.4067/S0717-95022011000300046 |
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Scielo Chile |
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language |
English |
topic |
Histochemistry analysis Morphometric analysis Vastus lateralis muscle Duchenne muscular dystrophy |
spellingShingle |
Histochemistry analysis Morphometric analysis Vastus lateralis muscle Duchenne muscular dystrophy Cavalcanti,George Maciel Oliveira,André de Sá Braga Assis,Thiago de Oliveira Chimelli,Leila Maria Cardão Medeiros,Paloma Lys de Mota,Diógenes Luís da Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD) |
description |
The aim of the study was to analyze the muscle fibers by histochemistry and morphometric methods from patients with Duchenne muscular dystrophy (DMD). Muscle biopsies were taken from the vastus lateralis muscle of five boys between 13 and 15-years of age, with clinical diagnosis of DMD. The histochemistry was performed using myofibrillar ATPases (9.6, 4.6 and 4.3). To morphometrical analysis a computerized semiautomatic system and software Image-Lab was used. ATPase staining showed atrophy of muscle fibers. Fibrosis and adipose deposition occurred in variable degree depending of muscular involvement. The morphometrical analysis showed an increase of size (percentage) to type I fiber than other types in all patients. Furthermore, the type I fiber had a larger cross-sectional area and mean diameter than type IIa and IIb fibers. Both histochemistry and morphometric analysis could be important tools for qualitative and quantitative diagnostics of muscle fibers attacked in this type of disease. |
author |
Cavalcanti,George Maciel Oliveira,André de Sá Braga Assis,Thiago de Oliveira Chimelli,Leila Maria Cardão Medeiros,Paloma Lys de Mota,Diógenes Luís da |
author_facet |
Cavalcanti,George Maciel Oliveira,André de Sá Braga Assis,Thiago de Oliveira Chimelli,Leila Maria Cardão Medeiros,Paloma Lys de Mota,Diógenes Luís da |
author_sort |
Cavalcanti,George Maciel |
title |
Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD) |
title_short |
Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD) |
title_full |
Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD) |
title_fullStr |
Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD) |
title_full_unstemmed |
Histochemistry and Morphometric Analysis of Muscle Fibers from Patients with Duchenne Muscular Dystrophy (DMD) |
title_sort |
histochemistry and morphometric analysis of muscle fibers from patients with duchenne muscular dystrophy (dmd) |
publisher |
Sociedad Chilena de Anatomía |
publishDate |
2011 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300046 |
work_keys_str_mv |
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