Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method

EGF family growth factors consists of growth factors, such as transforming growth factor (TGF)-a, heparin-binding EGF-like growth factor (HB-EGF), amphiregulin (AR) and epiregulin, autocrine growth factors in normal human keratinocytes. HB-EGF is mitogen for epithelial cells and like other members o...

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Autores principales:	Castrogiovanni,P, Mazzone,V, Imbesi,R
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Growth factors Keratinocytes Streptavidin-peroxidase conjugate
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400015
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spelling	oai:scielo:S0717-950220110004000152012-07-30Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate MethodCastrogiovanni,PMazzone,VImbesi,R Growth factors Keratinocytes Streptavidin-peroxidase conjugate EGF family growth factors consists of growth factors, such as transforming growth factor (TGF)-a, heparin-binding EGF-like growth factor (HB-EGF), amphiregulin (AR) and epiregulin, autocrine growth factors in normal human keratinocytes. HB-EGF is mitogen for epithelial cells and like other members of the EGF family, HB-EGF exerts its biological effects via interaction with the EGF receptor (EGFR). HB-EGF is an autocrine growth factor for human keratinocytes, and has a possible role as a paracrine growth factor for fibroblast. Our report concerning immunohistochemical localization of HB-EGF in normal skin by using the streptavidin-peroxidase (HRP) conjugate method, confirms previous data, revealing specific patterns of HB-EGF localization. Identification of HB-EGF in cells of epithelial origin suggests its autocrine and/or paracrine roles in epithelial cell maintenance. Our report especially wants to give a technical contribution, easy to manage and with evident results. A simple technique that does not require use of sophisticated equipment.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.4 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400015en10.4067/S0717-95022011000400015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Growth factors Keratinocytes Streptavidin-peroxidase conjugate
spellingShingle	Growth factors Keratinocytes Streptavidin-peroxidase conjugate Castrogiovanni,P Mazzone,V Imbesi,R Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method
description	EGF family growth factors consists of growth factors, such as transforming growth factor (TGF)-a, heparin-binding EGF-like growth factor (HB-EGF), amphiregulin (AR) and epiregulin, autocrine growth factors in normal human keratinocytes. HB-EGF is mitogen for epithelial cells and like other members of the EGF family, HB-EGF exerts its biological effects via interaction with the EGF receptor (EGFR). HB-EGF is an autocrine growth factor for human keratinocytes, and has a possible role as a paracrine growth factor for fibroblast. Our report concerning immunohistochemical localization of HB-EGF in normal skin by using the streptavidin-peroxidase (HRP) conjugate method, confirms previous data, revealing specific patterns of HB-EGF localization. Identification of HB-EGF in cells of epithelial origin suggests its autocrine and/or paracrine roles in epithelial cell maintenance. Our report especially wants to give a technical contribution, easy to manage and with evident results. A simple technique that does not require use of sophisticated equipment.
author	Castrogiovanni,P Mazzone,V Imbesi,R
author_facet	Castrogiovanni,P Mazzone,V Imbesi,R
author_sort	Castrogiovanni,P
title	Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method
title_short	Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method
title_full	Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method
title_fullStr	Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method
title_full_unstemmed	Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method
title_sort	immunolocalization of hb-egf in human skin by streptavidin-peroxidase (hrp) conjugate method
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400015
work_keys_str_mv	AT castrogiovannip immunolocalizationofhbegfinhumanskinbystreptavidinperoxidasehrpconjugatemethod AT mazzonev immunolocalizationofhbegfinhumanskinbystreptavidinperoxidasehrpconjugatemethod AT imbesir immunolocalizationofhbegfinhumanskinbystreptavidinperoxidasehrpconjugatemethod
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Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method

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