Histochemical and Morphological Characteristics of the Levator Ani Muscle in Rats

Histochemical and Morphological Characteristics of the Levator Ani Muscle in Rats

The current study aimed to analyze the histochemical and morphological characteristics of the levator ani muscle in rats. For this, we used 10 Wistar rats (5 males and 5 females), weighing between 200 and 765g. The animals were dissected fresh and in formalin for the levator ani muscle anatomical ob...

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Autores principales: do Carmo,Edna Maria, Miyaoka,Tatiana Midori, da Silva,Natália Denipoti, de Camargo,Marcela Regina, Rocha,Ana Paula Rodrigues, Pelai,Eliza Bizetti, Figueira,Jéssica Ibde Jaquiel, Fregonesi,Cristina Elena Prado Teles, Sato,José Takaiti, de Machado,Roberto Gameiro, de Souza,Wilson Machado
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2011
Materias:
Pelvic floor
Muscle skeletal
Physical therapy
Model animals
Muscle fibers
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400021
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spelling oai:scielo:S0717-950220110004000212012-07-30Histochemical and Morphological Characteristics of the Levator Ani Muscle in Ratsdo Carmo,Edna MariaMiyaoka,Tatiana Midorida Silva,Natália Denipotide Camargo,Marcela ReginaRocha,Ana Paula RodriguesPelai,Eliza BizettiFigueira,Jéssica Ibde JaquielFregonesi,Cristina Elena Prado TelesSato,José Takaitide Machado,Roberto Gameirode Souza,Wilson Machado Pelvic floor Muscle skeletal Physical therapy Model animals Muscle fibers The current study aimed to analyze the histochemical and morphological characteristics of the levator ani muscle in rats. For this, we used 10 Wistar rats (5 males and 5 females), weighing between 200 and 765g. The animals were dissected fresh and in formalin for the levator ani muscle anatomical observation. Muscle fragments were collected and frozen in n-Hexane previously cooled in liquid nitrogen. Then, the muscles were transferred to a microtome cryostat (HM 505 E Microm), being fixed in metal mounts with the adhesive Tissue Freezing Medium. Histological sections of 6.0µm were removed and subjected to HE staining. Other sections were subjected to NADH-TR and SDH reactions. After being dissected and fixed, the architecture of the female pelvic floor revealed the presence of two muscles: iliocaudalis and pubocaudalis. The anatomical inspection in male rats revealed, pronouncedly, the presence of the levator ani muscle: ischiocavernosus and bulbocavernous. We therefore observed a marked anatomical difference between animals of the same species, which does not occur with humans. The HE staining revealed muscular fibers with preserved morphology, contours ranging from polygonal to rounded, acidophilic cytoplasm, one or more peripheral nuclei with rounded shape and dense chromatin aspect. The fibers were organized in fascicles arranged by a dense connective tissue, the perimysium, and each fiber surrounded by the endomysium, composed of loose connective tissue. The sections subjected to NADH-TH and SDH, whose reactions show the activity of oxidative or glycolytic muscle fibers, allowed the identification of the two types of fiber. The fast-twitch fiber shows weaker reactivity, whereas the slow-twitch fiber has small diameter and intense reactivity, especially in the subsarcolemmal, presenting a highly oxidative metabolism. It was found that the pelvic floor muscles in rats are composed primarily by fast-twitch fibers, while in humans they are predominantly formed by slow-twitch fibers. Thus, models with these animals and various kinds of protocols for muscular training should be used cautiously.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.4 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400021en10.4067/S0717-95022011000400021
institution Scielo Chile
collection Scielo Chile
language English
topic Pelvic floor
Muscle skeletal
Physical therapy
Model animals
Muscle fibers
spellingShingle Pelvic floor
Muscle skeletal
Physical therapy
Model animals
Muscle fibers
do Carmo,Edna Maria
Miyaoka,Tatiana Midori
da Silva,Natália Denipoti
de Camargo,Marcela Regina
Rocha,Ana Paula Rodrigues
Pelai,Eliza Bizetti
Figueira,Jéssica Ibde Jaquiel
Fregonesi,Cristina Elena Prado Teles
Sato,José Takaiti
de Machado,Roberto Gameiro
de Souza,Wilson Machado
Histochemical and Morphological Characteristics of the Levator Ani Muscle in Rats
description The current study aimed to analyze the histochemical and morphological characteristics of the levator ani muscle in rats. For this, we used 10 Wistar rats (5 males and 5 females), weighing between 200 and 765g. The animals were dissected fresh and in formalin for the levator ani muscle anatomical observation. Muscle fragments were collected and frozen in n-Hexane previously cooled in liquid nitrogen. Then, the muscles were transferred to a microtome cryostat (HM 505 E Microm), being fixed in metal mounts with the adhesive Tissue Freezing Medium. Histological sections of 6.0µm were removed and subjected to HE staining. Other sections were subjected to NADH-TR and SDH reactions. After being dissected and fixed, the architecture of the female pelvic floor revealed the presence of two muscles: iliocaudalis and pubocaudalis. The anatomical inspection in male rats revealed, pronouncedly, the presence of the levator ani muscle: ischiocavernosus and bulbocavernous. We therefore observed a marked anatomical difference between animals of the same species, which does not occur with humans. The HE staining revealed muscular fibers with preserved morphology, contours ranging from polygonal to rounded, acidophilic cytoplasm, one or more peripheral nuclei with rounded shape and dense chromatin aspect. The fibers were organized in fascicles arranged by a dense connective tissue, the perimysium, and each fiber surrounded by the endomysium, composed of loose connective tissue. The sections subjected to NADH-TH and SDH, whose reactions show the activity of oxidative or glycolytic muscle fibers, allowed the identification of the two types of fiber. The fast-twitch fiber shows weaker reactivity, whereas the slow-twitch fiber has small diameter and intense reactivity, especially in the subsarcolemmal, presenting a highly oxidative metabolism. It was found that the pelvic floor muscles in rats are composed primarily by fast-twitch fibers, while in humans they are predominantly formed by slow-twitch fibers. Thus, models with these animals and various kinds of protocols for muscular training should be used cautiously.
author do Carmo,Edna Maria
Miyaoka,Tatiana Midori
da Silva,Natália Denipoti
de Camargo,Marcela Regina
Rocha,Ana Paula Rodrigues
Pelai,Eliza Bizetti
Figueira,Jéssica Ibde Jaquiel
Fregonesi,Cristina Elena Prado Teles
Sato,José Takaiti
de Machado,Roberto Gameiro
de Souza,Wilson Machado
author_facet do Carmo,Edna Maria
Miyaoka,Tatiana Midori
da Silva,Natália Denipoti
de Camargo,Marcela Regina
Rocha,Ana Paula Rodrigues
Pelai,Eliza Bizetti
Figueira,Jéssica Ibde Jaquiel
Fregonesi,Cristina Elena Prado Teles
Sato,José Takaiti
de Machado,Roberto Gameiro
de Souza,Wilson Machado
author_sort do Carmo,Edna Maria
title Histochemical and Morphological Characteristics of the Levator Ani Muscle in Rats
title_short Histochemical and Morphological Characteristics of the Levator Ani Muscle in Rats
title_full Histochemical and Morphological Characteristics of the Levator Ani Muscle in Rats
title_fullStr Histochemical and Morphological Characteristics of the Levator Ani Muscle in Rats
title_full_unstemmed Histochemical and Morphological Characteristics of the Levator Ani Muscle in Rats
title_sort histochemical and morphological characteristics of the levator ani muscle in rats
publisher Sociedad Chilena de Anatomía
publishDate 2011
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400021
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