Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy

The molecules that constitute the extracellular matrix are important in several functions related to tissue support and cell-cell, cell-extracellular matrix interaction. Among the macromolecules that constitute the mentioned matrix we find osteopontin, fibrinogen and collagen. The present study was...

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Autores principales:	Sanchis,E. G, Cristofolini,A. L, Taglialegna,A, Merkis,C. I
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2011
Materias:	Pig Placenta Osteopontin Fibrinogen Collagen
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400062
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spelling	oai:scielo:S0717-950220110004000622012-07-30Porcine Uterine and Placental Extracellular Matrix Molecules Throughout PregnancySanchis,E. GCristofolini,A. LTaglialegna,AMerkis,C. I Pig Placenta Osteopontin Fibrinogen Collagen The molecules that constitute the extracellular matrix are important in several functions related to tissue support and cell-cell, cell-extracellular matrix interaction. Among the macromolecules that constitute the mentioned matrix we find osteopontin, fibrinogen and collagen. The present study was undertaken to analyze the rol of osteopontin, fibrinogen and collagen in uterine-placental interface during normal porcine gestation. Uterine and placental tissues from crossbred gilts of 30 (n=5), 60 (n=5), 70 (n=5) and 114 (at term, n=5) days of gestation were used. Macroscopic analysis of the embryos/fetuses allowed us to determine their gestational age by means of the crown-rump lenght. Haematoxylin-Eosin and Masson's Trichrome dyes along with light microscopy were used to structure analysis of every selected period of gestation. A spacial and temporal study of osteopontin and fibrinogen was performed through immunohistochemical technique. Determination of collagen fibers was carried out through Picrosirius red technique and polarizing microscopy. Results were expressed as semi-quantitative. Higher expression of osteopontin was observed at early periods of gestation, mainly in uterine and placental villi, endometrial gland epithelium and histotroph. Fibrinogen expressed abundantly in fetal mesenchyme in every period analyzed and in fetal and maternal vessels at Day 70. Negative expression of collagen fibers was observed in villi, however increasing expression of thick fibers throughout pregnancy was detected in uterine stroma and myometrium. These results confirm the importance of osteopontin, fibrinogen and collagen in the support of uterine and placental structures and in the suitable maintenance of pregnancy.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.29 n.4 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400062en10.4067/S0717-95022011000400062
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pig Placenta Osteopontin Fibrinogen Collagen
spellingShingle	Pig Placenta Osteopontin Fibrinogen Collagen Sanchis,E. G Cristofolini,A. L Taglialegna,A Merkis,C. I Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy
description	The molecules that constitute the extracellular matrix are important in several functions related to tissue support and cell-cell, cell-extracellular matrix interaction. Among the macromolecules that constitute the mentioned matrix we find osteopontin, fibrinogen and collagen. The present study was undertaken to analyze the rol of osteopontin, fibrinogen and collagen in uterine-placental interface during normal porcine gestation. Uterine and placental tissues from crossbred gilts of 30 (n=5), 60 (n=5), 70 (n=5) and 114 (at term, n=5) days of gestation were used. Macroscopic analysis of the embryos/fetuses allowed us to determine their gestational age by means of the crown-rump lenght. Haematoxylin-Eosin and Masson's Trichrome dyes along with light microscopy were used to structure analysis of every selected period of gestation. A spacial and temporal study of osteopontin and fibrinogen was performed through immunohistochemical technique. Determination of collagen fibers was carried out through Picrosirius red technique and polarizing microscopy. Results were expressed as semi-quantitative. Higher expression of osteopontin was observed at early periods of gestation, mainly in uterine and placental villi, endometrial gland epithelium and histotroph. Fibrinogen expressed abundantly in fetal mesenchyme in every period analyzed and in fetal and maternal vessels at Day 70. Negative expression of collagen fibers was observed in villi, however increasing expression of thick fibers throughout pregnancy was detected in uterine stroma and myometrium. These results confirm the importance of osteopontin, fibrinogen and collagen in the support of uterine and placental structures and in the suitable maintenance of pregnancy.
author	Sanchis,E. G Cristofolini,A. L Taglialegna,A Merkis,C. I
author_facet	Sanchis,E. G Cristofolini,A. L Taglialegna,A Merkis,C. I
author_sort	Sanchis,E. G
title	Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy
title_short	Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy
title_full	Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy
title_fullStr	Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy
title_full_unstemmed	Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy
title_sort	porcine uterine and placental extracellular matrix molecules throughout pregnancy
publisher	Sociedad Chilena de Anatomía
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400062
work_keys_str_mv	AT sanchiseg porcineuterineandplacentalextracellularmatrixmoleculesthroughoutpregnancy AT cristofolinial porcineuterineandplacentalextracellularmatrixmoleculesthroughoutpregnancy AT taglialegnaa porcineuterineandplacentalextracellularmatrixmoleculesthroughoutpregnancy AT merkisci porcineuterineandplacentalextracellularmatrixmoleculesthroughoutpregnancy
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Porcine Uterine and Placental Extracellular Matrix Molecules Throughout Pregnancy

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