Role of Dilute Alcohol in the Removal of Fine Wrinkles from Paraffin Sections, a Histo-Technical Study

Histotechnology is concerned with processing and preparing of the body tissue in such a manner as to enable a satisfactory study of it. Section cutting is an integral part of histology and histopathology. It is an art by itself requiring skill and knowledge on the part of technician or the person wh...

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Autores principales:	Kumar,Naveen, Nayak B,Satheesha, Somayaji,S. N, Nayak,Sadananda
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Histological techniques Section cutting Microtomy Wrinkles Dilute alcohol
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100007
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spelling	oai:scielo:S0717-950220120001000072012-06-08Role of Dilute Alcohol in the Removal of Fine Wrinkles from Paraffin Sections, a Histo-Technical StudyKumar,NaveenNayak B,SatheeshaSomayaji,S. NNayak,Sadananda Histological techniques Section cutting Microtomy Wrinkles Dilute alcohol Histotechnology is concerned with processing and preparing of the body tissue in such a manner as to enable a satisfactory study of it. Section cutting is an integral part of histology and histopathology. It is an art by itself requiring skill and knowledge on the part of technician or the person who needs to do section cutting. In the routine method of preparing paraffin sections, it is often encountered by the presence of artefacts like fine wrinkles or folds. Attempts have been made to remove the wrinkles by floating the sections in the warm water bath. However this method has not been able to remove all the wrinkles from the sections. We have designed a simple and reliable method, in which the paraffin sections were floated over the ethyl alcohol diluted with water (1:15) before they were placed in the water bath. Through this method, we have been able to get the wrinkle free sections of superior quality. The advantage of our method is that, it is easy to prepare the dilute alcohol and is cost effective. This method can be used by the histology and pathology technicians and the researchers.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.1 20122012-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100007en10.4067/S0717-95022012000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Histological techniques Section cutting Microtomy Wrinkles Dilute alcohol
spellingShingle	Histological techniques Section cutting Microtomy Wrinkles Dilute alcohol Kumar,Naveen Nayak B,Satheesha Somayaji,S. N Nayak,Sadananda Role of Dilute Alcohol in the Removal of Fine Wrinkles from Paraffin Sections, a Histo-Technical Study
description	Histotechnology is concerned with processing and preparing of the body tissue in such a manner as to enable a satisfactory study of it. Section cutting is an integral part of histology and histopathology. It is an art by itself requiring skill and knowledge on the part of technician or the person who needs to do section cutting. In the routine method of preparing paraffin sections, it is often encountered by the presence of artefacts like fine wrinkles or folds. Attempts have been made to remove the wrinkles by floating the sections in the warm water bath. However this method has not been able to remove all the wrinkles from the sections. We have designed a simple and reliable method, in which the paraffin sections were floated over the ethyl alcohol diluted with water (1:15) before they were placed in the water bath. Through this method, we have been able to get the wrinkle free sections of superior quality. The advantage of our method is that, it is easy to prepare the dilute alcohol and is cost effective. This method can be used by the histology and pathology technicians and the researchers.
author	Kumar,Naveen Nayak B,Satheesha Somayaji,S. N Nayak,Sadananda
author_facet	Kumar,Naveen Nayak B,Satheesha Somayaji,S. N Nayak,Sadananda
author_sort	Kumar,Naveen
title	Role of Dilute Alcohol in the Removal of Fine Wrinkles from Paraffin Sections, a Histo-Technical Study
title_short	Role of Dilute Alcohol in the Removal of Fine Wrinkles from Paraffin Sections, a Histo-Technical Study
title_full	Role of Dilute Alcohol in the Removal of Fine Wrinkles from Paraffin Sections, a Histo-Technical Study
title_fullStr	Role of Dilute Alcohol in the Removal of Fine Wrinkles from Paraffin Sections, a Histo-Technical Study
title_full_unstemmed	Role of Dilute Alcohol in the Removal of Fine Wrinkles from Paraffin Sections, a Histo-Technical Study
title_sort	role of dilute alcohol in the removal of fine wrinkles from paraffin sections, a histo-technical study
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100007
work_keys_str_mv	AT kumarnaveen roleofdilutealcoholintheremovaloffinewrinklesfromparaffinsectionsahistotechnicalstudy AT nayakbsatheesha roleofdilutealcoholintheremovaloffinewrinklesfromparaffinsectionsahistotechnicalstudy AT somayajisn roleofdilutealcoholintheremovaloffinewrinklesfromparaffinsectionsahistotechnicalstudy AT nayaksadananda roleofdilutealcoholintheremovaloffinewrinklesfromparaffinsectionsahistotechnicalstudy
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Role of Dilute Alcohol in the Removal of Fine Wrinkles from Paraffin Sections, a Histo-Technical Study

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