Morphological Aspects of Spermatogenic Cells at Different Stagesof Sexual Maturation in Black Isogenic C57BL/6/Uni Mice

In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and...

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Autores principales: Martins,M. R. F. B, Silva,P. J. R, Martins,B. B, Zoca,S. M, Freitas,M. A, Batista,T. C
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2012
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200022
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spelling oai:scielo:S0717-950220120002000222012-10-03Morphological Aspects of Spermatogenic Cells at Different Stagesof Sexual Maturation in Black Isogenic C57BL/6/Uni MiceMartins,M. R. F. BSilva,P. J. RMartins,B. BZoca,S. MFreitas,M. ABatista,T. C Spermatogenesis Mouse Testes In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and leptotene was observed, whereas in the adluminal compartment Golgi phase spermatids we observed the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In Group III, 60 days old, it was found that seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. Therefore morphological evolution of germ cell testicular spermatids can be checked and recognized in its four phases: Golgi, cap, acrosome and maturation over the age of the animal.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.2 20122012-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200022en10.4067/S0717-95022012000200022
institution Scielo Chile
collection Scielo Chile
language English
topic Spermatogenesis
Mouse
Testes
spellingShingle Spermatogenesis
Mouse
Testes
Martins,M. R. F. B
Silva,P. J. R
Martins,B. B
Zoca,S. M
Freitas,M. A
Batista,T. C
Morphological Aspects of Spermatogenic Cells at Different Stagesof Sexual Maturation in Black Isogenic C57BL/6/Uni Mice
description In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and leptotene was observed, whereas in the adluminal compartment Golgi phase spermatids we observed the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In Group III, 60 days old, it was found that seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. Therefore morphological evolution of germ cell testicular spermatids can be checked and recognized in its four phases: Golgi, cap, acrosome and maturation over the age of the animal.
author Martins,M. R. F. B
Silva,P. J. R
Martins,B. B
Zoca,S. M
Freitas,M. A
Batista,T. C
author_facet Martins,M. R. F. B
Silva,P. J. R
Martins,B. B
Zoca,S. M
Freitas,M. A
Batista,T. C
author_sort Martins,M. R. F. B
title Morphological Aspects of Spermatogenic Cells at Different Stagesof Sexual Maturation in Black Isogenic C57BL/6/Uni Mice
title_short Morphological Aspects of Spermatogenic Cells at Different Stagesof Sexual Maturation in Black Isogenic C57BL/6/Uni Mice
title_full Morphological Aspects of Spermatogenic Cells at Different Stagesof Sexual Maturation in Black Isogenic C57BL/6/Uni Mice
title_fullStr Morphological Aspects of Spermatogenic Cells at Different Stagesof Sexual Maturation in Black Isogenic C57BL/6/Uni Mice
title_full_unstemmed Morphological Aspects of Spermatogenic Cells at Different Stagesof Sexual Maturation in Black Isogenic C57BL/6/Uni Mice
title_sort morphological aspects of spermatogenic cells at different stagesof sexual maturation in black isogenic c57bl/6/uni mice
publisher Sociedad Chilena de Anatomía
publishDate 2012
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200022
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