Repression of Ppargcla Gene in Liver of Hyperglycemic Rats Induced with High Fat Diet Combined with Streptozotocin

Type 2 diabetes mellitus implies deregulation of multiple metabolic processes, being the maintenance of glycemia one of the most important. Many genes are involved in the deregulation of this particular process. Therefore, the aim of this study was to evaluate gene expression of genes related to typ...

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Autores principales:	Hernández,Alfonso, Curi,Rui, Salazar,Luis A
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Gene Expression Hyperglycemia Ppargcla
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200047
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spelling	oai:scielo:S0717-950220120002000472012-10-03Repression of Ppargcla Gene in Liver of Hyperglycemic Rats Induced with High Fat Diet Combined with StreptozotocinHernández,AlfonsoCuri,RuiSalazar,Luis A Gene Expression Hyperglycemia Ppargcla Type 2 diabetes mellitus implies deregulation of multiple metabolic processes, being the maintenance of glycemia one of the most important. Many genes are involved in the deregulation of this particular process. Therefore, the aim of this study was to evaluate gene expression of genes related to type 2 diabetes mellitus, in the liver and pancreas of rats with hyperglycemia induced by high fat diet along with a low single dose of streptozotocin. Ahsg and Ppargc1a genes were studied in liver, whereas Kcnj11 and Slc2a2 genes were analyzed in pancreas. For this purpose, 210-240 g female rats were fed a high fat diet or a control diet for three weeks. At day 14, animals fed with high fat diet were injected with a single low dose of streptozotocin (35 mg/kg) and the control group rats were injected only with the vehicle. Plasmatic glucose, triglycerides and total cholesterol levels were measured at the beginning, day 14 and end of treatment. Body weight was also measured. Once the treatment was complete, rats were appropriately euthanized and then, pancreas and liver were surgically removed and frozen in liquid nitrogen. Total RNA was isolated using TRIzol reagent, treated with DNase I and reversely transcribed to cDNA. Gene expression analysis was performed using SYBR Green Real time PCR and comparative Cq method, using three reference genes. Rats fed with high fat diet and treated with streptozotocin showed higher values of plasmatic glucose (17.09 ± 0.43 vs. 5.91 ± 0.29 mmol/L, p < 0.01) and a minor expression of Ppargc1a versus the control group (2-fold less expressed, p < 0.05) in liver. We conclude that repression of Ppargc1a gene may be an important process in the establishment of chronic hyperglycemia, probably through deregulation of hepatic gluconeogenesis. However, further studies need to be performed in order to clarify the role of Ppargc1a deregulation in liver glucose homeostasis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.2 20122012-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200047en10.4067/S0717-95022012000200047
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gene Expression Hyperglycemia Ppargcla
spellingShingle	Gene Expression Hyperglycemia Ppargcla Hernández,Alfonso Curi,Rui Salazar,Luis A Repression of Ppargcla Gene in Liver of Hyperglycemic Rats Induced with High Fat Diet Combined with Streptozotocin
description	Type 2 diabetes mellitus implies deregulation of multiple metabolic processes, being the maintenance of glycemia one of the most important. Many genes are involved in the deregulation of this particular process. Therefore, the aim of this study was to evaluate gene expression of genes related to type 2 diabetes mellitus, in the liver and pancreas of rats with hyperglycemia induced by high fat diet along with a low single dose of streptozotocin. Ahsg and Ppargc1a genes were studied in liver, whereas Kcnj11 and Slc2a2 genes were analyzed in pancreas. For this purpose, 210-240 g female rats were fed a high fat diet or a control diet for three weeks. At day 14, animals fed with high fat diet were injected with a single low dose of streptozotocin (35 mg/kg) and the control group rats were injected only with the vehicle. Plasmatic glucose, triglycerides and total cholesterol levels were measured at the beginning, day 14 and end of treatment. Body weight was also measured. Once the treatment was complete, rats were appropriately euthanized and then, pancreas and liver were surgically removed and frozen in liquid nitrogen. Total RNA was isolated using TRIzol reagent, treated with DNase I and reversely transcribed to cDNA. Gene expression analysis was performed using SYBR Green Real time PCR and comparative Cq method, using three reference genes. Rats fed with high fat diet and treated with streptozotocin showed higher values of plasmatic glucose (17.09 ± 0.43 vs. 5.91 ± 0.29 mmol/L, p < 0.01) and a minor expression of Ppargc1a versus the control group (2-fold less expressed, p < 0.05) in liver. We conclude that repression of Ppargc1a gene may be an important process in the establishment of chronic hyperglycemia, probably through deregulation of hepatic gluconeogenesis. However, further studies need to be performed in order to clarify the role of Ppargc1a deregulation in liver glucose homeostasis.
author	Hernández,Alfonso Curi,Rui Salazar,Luis A
author_facet	Hernández,Alfonso Curi,Rui Salazar,Luis A
author_sort	Hernández,Alfonso
title	Repression of Ppargcla Gene in Liver of Hyperglycemic Rats Induced with High Fat Diet Combined with Streptozotocin
title_short	Repression of Ppargcla Gene in Liver of Hyperglycemic Rats Induced with High Fat Diet Combined with Streptozotocin
title_full	Repression of Ppargcla Gene in Liver of Hyperglycemic Rats Induced with High Fat Diet Combined with Streptozotocin
title_fullStr	Repression of Ppargcla Gene in Liver of Hyperglycemic Rats Induced with High Fat Diet Combined with Streptozotocin
title_full_unstemmed	Repression of Ppargcla Gene in Liver of Hyperglycemic Rats Induced with High Fat Diet Combined with Streptozotocin
title_sort	repression of ppargcla gene in liver of hyperglycemic rats induced with high fat diet combined with streptozotocin
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200047
work_keys_str_mv	AT hernandezalfonso repressionofppargclageneinliverofhyperglycemicratsinducedwithhighfatdietcombinedwithstreptozotocin AT curirui repressionofppargclageneinliverofhyperglycemicratsinducedwithhighfatdietcombinedwithstreptozotocin AT salazarluisa repressionofppargclageneinliverofhyperglycemicratsinducedwithhighfatdietcombinedwithstreptozotocin
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Repression of Ppargcla Gene in Liver of Hyperglycemic Rats Induced with High Fat Diet Combined with Streptozotocin

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