Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

Diseases caused by avian paramyxovirus (APMV) occur in commercial, captive and wild birds worldwide, demonstrating the significant economic and ecological importance of these agents. Paramyxoviruses belong to the Paramyxoviridae family, Paramyxovirinae subfamily and Avulavirus genus. During the peri...

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Autores principales:	Catroxo,M. H. B, Martins,A. M. C. P. F, Petrella,S, Milanelo,L, Aschar,M, Souza,F, Nastari,B.D.B, Souza,R.B
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Paramyxoviruses Avian Transmission Electron Microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200062
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spelling	oai:scielo:S0717-950220120002000622012-10-03Avian Paramyxoviruses: Detection by Transmission Electron Microscopy TechniquesCatroxo,M. H. BMartins,A. M. C. P. FPetrella,SMilanelo,LAschar,MSouza,FNastari,B.D.BSouza,R.B Paramyxoviruses Avian Transmission Electron Microscopy Diseases caused by avian paramyxovirus (APMV) occur in commercial, captive and wild birds worldwide, demonstrating the significant economic and ecological importance of these agents. Paramyxoviruses belong to the Paramyxoviridae family, Paramyxovirinae subfamily and Avulavirus genus. During the period 2000 to 2011, stool and small intestine samples of 1647 birds species were sent to the Laboratory of Electron Microscopy, Biological Institute of São Paulo, Brazil, for diagnosis of viral agents. The samples were processed by negative staining (rapid preparation) and resin embedding techniques. Under the transmission electron microscope by negative staining technique, in 294 (17.8%) samples of 1647 were visualized paramyxovirus particles pleomorphic, roughly spherical or filamentous, measuring 100 to 500 nm of diameter containing an envelope covered with spikes and characteristic helical herring-bone-like nucleocapsid measuring 15 to 20 nm in diameter. Ultrathin sections of the small intestine fragments revealed the presence of amorphous granular intracytoplasmic inclusions surrounded by membrane and containing viral nucleocapsid measuring 10-14 nm in diameter. Immature particles budding from cell membranes, pleomorphic, spherical and tubular particles containing viral nucleocapsid strands, and the complete particles measured up to 170 nm in diameter were seen in the cytoplasm. Intranuclear inclusions containing viral nucleocapsid were also visualized. Nuclei showed a marginalized chromatin.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.2 20122012-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200062en10.4067/S0717-95022012000200062
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Paramyxoviruses Avian Transmission Electron Microscopy
spellingShingle	Paramyxoviruses Avian Transmission Electron Microscopy Catroxo,M. H. B Martins,A. M. C. P. F Petrella,S Milanelo,L Aschar,M Souza,F Nastari,B.D.B Souza,R.B Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques
description	Diseases caused by avian paramyxovirus (APMV) occur in commercial, captive and wild birds worldwide, demonstrating the significant economic and ecological importance of these agents. Paramyxoviruses belong to the Paramyxoviridae family, Paramyxovirinae subfamily and Avulavirus genus. During the period 2000 to 2011, stool and small intestine samples of 1647 birds species were sent to the Laboratory of Electron Microscopy, Biological Institute of São Paulo, Brazil, for diagnosis of viral agents. The samples were processed by negative staining (rapid preparation) and resin embedding techniques. Under the transmission electron microscope by negative staining technique, in 294 (17.8%) samples of 1647 were visualized paramyxovirus particles pleomorphic, roughly spherical or filamentous, measuring 100 to 500 nm of diameter containing an envelope covered with spikes and characteristic helical herring-bone-like nucleocapsid measuring 15 to 20 nm in diameter. Ultrathin sections of the small intestine fragments revealed the presence of amorphous granular intracytoplasmic inclusions surrounded by membrane and containing viral nucleocapsid measuring 10-14 nm in diameter. Immature particles budding from cell membranes, pleomorphic, spherical and tubular particles containing viral nucleocapsid strands, and the complete particles measured up to 170 nm in diameter were seen in the cytoplasm. Intranuclear inclusions containing viral nucleocapsid were also visualized. Nuclei showed a marginalized chromatin.
author	Catroxo,M. H. B Martins,A. M. C. P. F Petrella,S Milanelo,L Aschar,M Souza,F Nastari,B.D.B Souza,R.B
author_facet	Catroxo,M. H. B Martins,A. M. C. P. F Petrella,S Milanelo,L Aschar,M Souza,F Nastari,B.D.B Souza,R.B
author_sort	Catroxo,M. H. B
title	Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques
title_short	Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques
title_full	Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques
title_fullStr	Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques
title_full_unstemmed	Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques
title_sort	avian paramyxoviruses: detection by transmission electron microscopy techniques
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200062
work_keys_str_mv	AT catroxomhb avianparamyxovirusesdetectionbytransmissionelectronmicroscopytechniques AT martinsamcpf avianparamyxovirusesdetectionbytransmissionelectronmicroscopytechniques AT petrellas avianparamyxovirusesdetectionbytransmissionelectronmicroscopytechniques AT milanelol avianparamyxovirusesdetectionbytransmissionelectronmicroscopytechniques AT ascharm avianparamyxovirusesdetectionbytransmissionelectronmicroscopytechniques AT souzaf avianparamyxovirusesdetectionbytransmissionelectronmicroscopytechniques AT nastaribdb avianparamyxovirusesdetectionbytransmissionelectronmicroscopytechniques AT souzarb avianparamyxovirusesdetectionbytransmissionelectronmicroscopytechniques
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Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

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