Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques

In this study thirty shrimp samples from commercial marine shrimp (L. vannamei) farms of southern region of Brazil were obtained. Hepatopancreas and shell scrapings fragments collected in these animals were processed by transmission electron microscopy using negative staining (rapid preparation), im...

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Autores principales:	Hipolito,M, Catroxo,M. H. B, Martins,A. M. C. R. P. F, Melo,N.A, Pituco,E. M, Galleti,N.T.C, Ranzani-Paiva,M. J. T, Mouriño,J.L.P, Ferreira,C.M
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	White spot syndrome virus Negative staining Immunoelectron microscopy Immunocytochemistry Marine shrimp Litopenaeus vannamei
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200067
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spelling	oai:scielo:S0717-950220120002000672012-10-03Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry TechniquesHipolito,MCatroxo,M. H. BMartins,A. M. C. R. P. FMelo,N.APituco,E. MGalleti,N.T.CRanzani-Paiva,M. J. TMouriño,J.L.PFerreira,C.M White spot syndrome virus Negative staining Immunoelectron microscopy Immunocytochemistry Marine shrimp Litopenaeus vannamei In this study thirty shrimp samples from commercial marine shrimp (L. vannamei) farms of southern region of Brazil were obtained. Hepatopancreas and shell scrapings fragments collected in these animals were processed by transmission electron microscopy using negative staining (rapid preparation), immunoelectron microscopy and immunocytochemistry (immunolabelling with colloidal gold particles) techniques. On the transmission electron microscopy a great number of white spot virus particles, ovoid or bacilliform-to-ellipsoid, measured 230-290 nm in length and 80-160 nm in diameter with intra-nuclear projections were visualized by the negative staining technique in 27 (90%) out of 30 samples examined. Using immunoelectron microscopy technique, the anti-VP 664 serum agllutinated a large number of particles formed by antigen-antibody interaction. In the immunocytochemistry technique, the antigen-antibody reaction was styrongly marked by the particles of colloidal gold over the virus. Notably, this is the first report, to our knowledge, describing use of these microscopy techniques to study Brazilian L. vannamei marine shrimp samples; moreover, this methodology also appears to be a viable complementary tool for diagnosing the presence of the white spot virus within shrimp tissues. Importantly, these are the first photoelectron micrographs of the WSSV in Brazil.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.2 20122012-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200067en10.4067/S0717-95022012000200067
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	White spot syndrome virus Negative staining Immunoelectron microscopy Immunocytochemistry Marine shrimp Litopenaeus vannamei
spellingShingle	White spot syndrome virus Negative staining Immunoelectron microscopy Immunocytochemistry Marine shrimp Litopenaeus vannamei Hipolito,M Catroxo,M. H. B Martins,A. M. C. R. P. F Melo,N.A Pituco,E. M Galleti,N.T.C Ranzani-Paiva,M. J. T Mouriño,J.L.P Ferreira,C.M Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques
description	In this study thirty shrimp samples from commercial marine shrimp (L. vannamei) farms of southern region of Brazil were obtained. Hepatopancreas and shell scrapings fragments collected in these animals were processed by transmission electron microscopy using negative staining (rapid preparation), immunoelectron microscopy and immunocytochemistry (immunolabelling with colloidal gold particles) techniques. On the transmission electron microscopy a great number of white spot virus particles, ovoid or bacilliform-to-ellipsoid, measured 230-290 nm in length and 80-160 nm in diameter with intra-nuclear projections were visualized by the negative staining technique in 27 (90%) out of 30 samples examined. Using immunoelectron microscopy technique, the anti-VP 664 serum agllutinated a large number of particles formed by antigen-antibody interaction. In the immunocytochemistry technique, the antigen-antibody reaction was styrongly marked by the particles of colloidal gold over the virus. Notably, this is the first report, to our knowledge, describing use of these microscopy techniques to study Brazilian L. vannamei marine shrimp samples; moreover, this methodology also appears to be a viable complementary tool for diagnosing the presence of the white spot virus within shrimp tissues. Importantly, these are the first photoelectron micrographs of the WSSV in Brazil.
author	Hipolito,M Catroxo,M. H. B Martins,A. M. C. R. P. F Melo,N.A Pituco,E. M Galleti,N.T.C Ranzani-Paiva,M. J. T Mouriño,J.L.P Ferreira,C.M
author_facet	Hipolito,M Catroxo,M. H. B Martins,A. M. C. R. P. F Melo,N.A Pituco,E. M Galleti,N.T.C Ranzani-Paiva,M. J. T Mouriño,J.L.P Ferreira,C.M
author_sort	Hipolito,M
title	Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques
title_short	Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques
title_full	Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques
title_fullStr	Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques
title_full_unstemmed	Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques
title_sort	detection of white spot syndrome virus in brazil using negative staining, immunoelectron microscopy and immunocytochemistry techniques
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200067
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Detection of White Spot Syndrome Virus in Brazil using Negative Staining, Immunoelectron Microscopy and Immunocytochemistry Techniques

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