Analysis of the NMDA in Focal Cerebral Ischemia in Rats

NMDAR (N-methyl-D-aspartate receptor) is one subtype of ionotrophic glutamate receptor which is extensively distributed in the central nervous system (CNS). In the mammalian CNS, NMDAR serves prominent roles in the pathophysiologic process of cerebral ischemia. This study aimed to investigate the pa...

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Autores principales:	Tirapelli,D. P. C, Carlotti Jr,C. G, Leite,J. P, Lizarte,F. S. N, Tirapelli,L. F, Colli,B. O
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	NMDA expression Cerebral ischemia Neuroprotection hypothermia and ketoprofen
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300035
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spelling	oai:scielo:S0717-950220120003000352013-02-20Analysis of the NMDA in Focal Cerebral Ischemia in RatsTirapelli,D. P. CCarlotti Jr,C. GLeite,J. PLizarte,F. S. NTirapelli,L. FColli,B. O NMDA expression Cerebral ischemia Neuroprotection hypothermia and ketoprofen NMDAR (N-methyl-D-aspartate receptor) is one subtype of ionotrophic glutamate receptor which is extensively distributed in the central nervous system (CNS). In the mammalian CNS, NMDAR serves prominent roles in the pathophysiologic process of cerebral ischemia. This study aimed to investigate the pattern of expression of protein and gene of the excitatory neurotransmitter NMDAR in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. 120 rats were randomly divided into 6 groups (20 animals each): control - no surgery; sham - simulation of surgery; ischemic - focal ischemia for 1 hour, without reperfusion; ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. Ten animals from each experimental group were used to establish the volume of infarct. Transient focal cerebral ischemia was obtained in rats by occlusion of the middle cerebral artery with an intraluminal suture. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the protein and gene NMDA were evaluated by immunohistochemistry and quantitative real-time PCR, respectively. Increases in the protein and gene NMDA receptor in the ischemics areas were observed and these increases were reduced by hypothermia and ketoprofen. The increase in the NMDA receptor protein and gene expression observed in the ischemic animals was reduced by neuroprotection (hypothermia and ketoprofen). The NMDA receptor increases in the ischemic area suggests that the NMDA mediated neuroexcitotoxicity plays an important role in cell death and that the neuroprotective effect of both, hypothermia and ketoprofen is directly involved with the NMDA.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.3 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300035en10.4067/S0717-95022012000300035
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	NMDA expression Cerebral ischemia Neuroprotection hypothermia and ketoprofen
spellingShingle	NMDA expression Cerebral ischemia Neuroprotection hypothermia and ketoprofen Tirapelli,D. P. C Carlotti Jr,C. G Leite,J. P Lizarte,F. S. N Tirapelli,L. F Colli,B. O Analysis of the NMDA in Focal Cerebral Ischemia in Rats
description	NMDAR (N-methyl-D-aspartate receptor) is one subtype of ionotrophic glutamate receptor which is extensively distributed in the central nervous system (CNS). In the mammalian CNS, NMDAR serves prominent roles in the pathophysiologic process of cerebral ischemia. This study aimed to investigate the pattern of expression of protein and gene of the excitatory neurotransmitter NMDAR in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. 120 rats were randomly divided into 6 groups (20 animals each): control - no surgery; sham - simulation of surgery; ischemic - focal ischemia for 1 hour, without reperfusion; ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. Ten animals from each experimental group were used to establish the volume of infarct. Transient focal cerebral ischemia was obtained in rats by occlusion of the middle cerebral artery with an intraluminal suture. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the protein and gene NMDA were evaluated by immunohistochemistry and quantitative real-time PCR, respectively. Increases in the protein and gene NMDA receptor in the ischemics areas were observed and these increases were reduced by hypothermia and ketoprofen. The increase in the NMDA receptor protein and gene expression observed in the ischemic animals was reduced by neuroprotection (hypothermia and ketoprofen). The NMDA receptor increases in the ischemic area suggests that the NMDA mediated neuroexcitotoxicity plays an important role in cell death and that the neuroprotective effect of both, hypothermia and ketoprofen is directly involved with the NMDA.
author	Tirapelli,D. P. C Carlotti Jr,C. G Leite,J. P Lizarte,F. S. N Tirapelli,L. F Colli,B. O
author_facet	Tirapelli,D. P. C Carlotti Jr,C. G Leite,J. P Lizarte,F. S. N Tirapelli,L. F Colli,B. O
author_sort	Tirapelli,D. P. C
title	Analysis of the NMDA in Focal Cerebral Ischemia in Rats
title_short	Analysis of the NMDA in Focal Cerebral Ischemia in Rats
title_full	Analysis of the NMDA in Focal Cerebral Ischemia in Rats
title_fullStr	Analysis of the NMDA in Focal Cerebral Ischemia in Rats
title_full_unstemmed	Analysis of the NMDA in Focal Cerebral Ischemia in Rats
title_sort	analysis of the nmda in focal cerebral ischemia in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300035
work_keys_str_mv	AT tirapellidpc analysisofthenmdainfocalcerebralischemiainrats AT carlottijrcg analysisofthenmdainfocalcerebralischemiainrats AT leitejp analysisofthenmdainfocalcerebralischemiainrats AT lizartefsn analysisofthenmdainfocalcerebralischemiainrats AT tirapellilf analysisofthenmdainfocalcerebralischemiainrats AT collibo analysisofthenmdainfocalcerebralischemiainrats
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Analysis of the NMDA in Focal Cerebral Ischemia in Rats

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