Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis

The aim was to analyze the protein expression of apoptotic genes caspase-3, caspase-8 and bcl-2 with the immunohistochemistry technique, correlating with tumor grade (I, II and III) and with the patient survival in order to understand the basic mechanism of tumoral transformation. The immunohistoche...

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Autores principales:	Novais,P. C, de Paula,A. A. P, Reis,R. B, Barione,D. F, Franqueiro,A. G, Rodrigues Jr,A. A, Lizarte,F. S. N, de Carvalho,C. A. M, Tirapelli,D. P. C, Peria,F. M, Saggioro,F. P, Tucci Jr,S, Cologna,A. J, Martins,A. C. P, Tirapelli,L. F
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	bcl-2 Caspase-3 Caspase-8 Penile cancer Apoptosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300043
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spelling	oai:scielo:S0717-950220120003000432013-02-20Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof PenisNovais,P. Cde Paula,A. A. PReis,R. BBarione,D. FFranqueiro,A. GRodrigues Jr,A. ALizarte,F. S. Nde Carvalho,C. A. MTirapelli,D. P. CPeria,F. MSaggioro,F. PTucci Jr,SCologna,A. JMartins,A. C. PTirapelli,L. F bcl-2 Caspase-3 Caspase-8 Penile cancer Apoptosis The aim was to analyze the protein expression of apoptotic genes caspase-3, caspase-8 and bcl-2 with the immunohistochemistry technique, correlating with tumor grade (I, II and III) and with the patient survival in order to understand the basic mechanism of tumoral transformation. The immunohistochemistry reactions on 50 samples of squamous cell carcinoma were carried out with the avidin-biotin immunoperoxidase method and antigen recovery. The analyses were made using the graduation method "in crosses" (0 to 4 crosses - no stain to more than 75% of positives cells) and in categories (low, intermediate, high) of the cytoplasm immunoreactivity of the epidermoid penile carcinoma cells. It was observed a statistically significant difference when the expression of caspase-3 were compared with the grades I and II of the tumor (p=0.0010) and when comparing the patient survival with the grades I and II of the tumor (p=0.0212). The protein bcl-2 was more expressed than caspase-3 and caspase-8 proteins, suggesting that the apoptotic rate in this carcinoma is low. The higher expression of the anti-apoptotic protein bcl-2 suggests a higher preservation of the tumoral cells.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.3 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300043en10.4067/S0717-95022012000300043
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bcl-2 Caspase-3 Caspase-8 Penile cancer Apoptosis
spellingShingle	bcl-2 Caspase-3 Caspase-8 Penile cancer Apoptosis Novais,P. C de Paula,A. A. P Reis,R. B Barione,D. F Franqueiro,A. G Rodrigues Jr,A. A Lizarte,F. S. N de Carvalho,C. A. M Tirapelli,D. P. C Peria,F. M Saggioro,F. P Tucci Jr,S Cologna,A. J Martins,A. C. P Tirapelli,L. F Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis
description	The aim was to analyze the protein expression of apoptotic genes caspase-3, caspase-8 and bcl-2 with the immunohistochemistry technique, correlating with tumor grade (I, II and III) and with the patient survival in order to understand the basic mechanism of tumoral transformation. The immunohistochemistry reactions on 50 samples of squamous cell carcinoma were carried out with the avidin-biotin immunoperoxidase method and antigen recovery. The analyses were made using the graduation method "in crosses" (0 to 4 crosses - no stain to more than 75% of positives cells) and in categories (low, intermediate, high) of the cytoplasm immunoreactivity of the epidermoid penile carcinoma cells. It was observed a statistically significant difference when the expression of caspase-3 were compared with the grades I and II of the tumor (p=0.0010) and when comparing the patient survival with the grades I and II of the tumor (p=0.0212). The protein bcl-2 was more expressed than caspase-3 and caspase-8 proteins, suggesting that the apoptotic rate in this carcinoma is low. The higher expression of the anti-apoptotic protein bcl-2 suggests a higher preservation of the tumoral cells.
author	Novais,P. C de Paula,A. A. P Reis,R. B Barione,D. F Franqueiro,A. G Rodrigues Jr,A. A Lizarte,F. S. N de Carvalho,C. A. M Tirapelli,D. P. C Peria,F. M Saggioro,F. P Tucci Jr,S Cologna,A. J Martins,A. C. P Tirapelli,L. F
author_facet	Novais,P. C de Paula,A. A. P Reis,R. B Barione,D. F Franqueiro,A. G Rodrigues Jr,A. A Lizarte,F. S. N de Carvalho,C. A. M Tirapelli,D. P. C Peria,F. M Saggioro,F. P Tucci Jr,S Cologna,A. J Martins,A. C. P Tirapelli,L. F
author_sort	Novais,P. C
title	Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis
title_short	Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis
title_full	Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis
title_fullStr	Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis
title_full_unstemmed	Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis
title_sort	immunohistochemistry analysis of proteins related with apoptosis as prognostic factor inepidermoid carcinomaof penis
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300043
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Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis

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