Determination of the Species from Skeletal Remains Through Histomorphometric Evaluation and Discriminant Analysis
In situations where the skeletal remains found are too fragmented, it is necessary to assess the human origin of such remains; for this purpose, various parameters are used, both anatomical and histological. The objective of the present study is to evaluate the various histomorphometric parameters t...
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Sociedad Chilena de Anatomía
2012
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oai:scielo:S0717-950220120003000442013-02-20Determination of the Species from Skeletal Remains Through Histomorphometric Evaluation and Discriminant AnalysisMorales,Juan-PabloRoa H,IgnacioZavando,DanielaSuazo Galdames,Iván Bone Forensic analysis Determination of species Osteon compact bone Haversian canal In situations where the skeletal remains found are too fragmented, it is necessary to assess the human origin of such remains; for this purpose, various parameters are used, both anatomical and histological. The objective of the present study is to evaluate the various histomorphometric parameters to differentiate human from non-human bones, to further construct discriminatory functions that allow interspecies classification. Tibia bones sections from human, bovine, pig, hen, cat, and dog species were used, processed through conventional histological techniques and observed under the microscope with a 40x magnification, analyzing the Haversian Canal Density parameters by mm2, Diameter of the Haversian Canal and the Diameter of the Haversian System which were compared through one way ANOVA with Scheffé post test, p<0.05. Subsequently, the discriminatory functions were constructed for each species and the percentage of well-diagnosed cases was determined. Meaningful differences were found in the parameters analyzed; the discriminatory functions allowed to correctly classify 88.5% of the cases. Our results suggest that it is possible to differentiate human skeletal remains from non-human through the observation of their histological characteristics and histomorphometric parameters, but interspecies differentiation requires a more complex analysis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.3 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300044en10.4067/S0717-95022012000300044 |
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Scielo Chile |
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English |
topic |
Bone Forensic analysis Determination of species Osteon compact bone Haversian canal |
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Bone Forensic analysis Determination of species Osteon compact bone Haversian canal Morales,Juan-Pablo Roa H,Ignacio Zavando,Daniela Suazo Galdames,Iván Determination of the Species from Skeletal Remains Through Histomorphometric Evaluation and Discriminant Analysis |
description |
In situations where the skeletal remains found are too fragmented, it is necessary to assess the human origin of such remains; for this purpose, various parameters are used, both anatomical and histological. The objective of the present study is to evaluate the various histomorphometric parameters to differentiate human from non-human bones, to further construct discriminatory functions that allow interspecies classification. Tibia bones sections from human, bovine, pig, hen, cat, and dog species were used, processed through conventional histological techniques and observed under the microscope with a 40x magnification, analyzing the Haversian Canal Density parameters by mm2, Diameter of the Haversian Canal and the Diameter of the Haversian System which were compared through one way ANOVA with Scheffé post test, p<0.05. Subsequently, the discriminatory functions were constructed for each species and the percentage of well-diagnosed cases was determined. Meaningful differences were found in the parameters analyzed; the discriminatory functions allowed to correctly classify 88.5% of the cases. Our results suggest that it is possible to differentiate human skeletal remains from non-human through the observation of their histological characteristics and histomorphometric parameters, but interspecies differentiation requires a more complex analysis. |
author |
Morales,Juan-Pablo Roa H,Ignacio Zavando,Daniela Suazo Galdames,Iván |
author_facet |
Morales,Juan-Pablo Roa H,Ignacio Zavando,Daniela Suazo Galdames,Iván |
author_sort |
Morales,Juan-Pablo |
title |
Determination of the Species from Skeletal Remains Through Histomorphometric Evaluation and Discriminant Analysis |
title_short |
Determination of the Species from Skeletal Remains Through Histomorphometric Evaluation and Discriminant Analysis |
title_full |
Determination of the Species from Skeletal Remains Through Histomorphometric Evaluation and Discriminant Analysis |
title_fullStr |
Determination of the Species from Skeletal Remains Through Histomorphometric Evaluation and Discriminant Analysis |
title_full_unstemmed |
Determination of the Species from Skeletal Remains Through Histomorphometric Evaluation and Discriminant Analysis |
title_sort |
determination of the species from skeletal remains through histomorphometric evaluation and discriminant analysis |
publisher |
Sociedad Chilena de Anatomía |
publishDate |
2012 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300044 |
work_keys_str_mv |
AT moralesjuanpablo determinationofthespeciesfromskeletalremainsthroughhistomorphometricevaluationanddiscriminantanalysis AT roahignacio determinationofthespeciesfromskeletalremainsthroughhistomorphometricevaluationanddiscriminantanalysis AT zavandodaniela determinationofthespeciesfromskeletalremainsthroughhistomorphometricevaluationanddiscriminantanalysis AT suazogaldamesivan determinationofthespeciesfromskeletalremainsthroughhistomorphometricevaluationanddiscriminantanalysis |
_version_ |
1718444794568507392 |