Contemporary Staining of Arterial System with Cadaver Fixation: An Experimental Study

Although many methods are investigated, cadavers have unique role for education of gross anatomy. This study was performed to make cadaveric based gross anatomy more enjoyable and decrease the time needed for achievement of arterial system for teachers and learners. The adult rabbits in control (n=3...

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Autores principales:	Ghorbani,Rostam, Tavakol,Zahra, Ghanbari,Ali
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Artery Cadaver Eosin Packed cells Staining
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300052
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spelling	oai:scielo:S0717-950220120003000522013-02-20Contemporary Staining of Arterial System with Cadaver Fixation: An Experimental StudyGhorbani,RostamTavakol,ZahraGhanbari,Ali Artery Cadaver Eosin Packed cells Staining Although many methods are investigated, cadavers have unique role for education of gross anatomy. This study was performed to make cadaveric based gross anatomy more enjoyable and decrease the time needed for achievement of arterial system for teachers and learners. The adult rabbits in control (n=3) and experimental groups (n=9) sacrificed following deep anesthesia. The samples were fixed by injection of fixative solution to the left ventricle and the corps dropped to the traditional fixative liquid for at least 1 month. The injected fixative solution of experimental group contained stained human red blood cells. The red blood cells stained by eosin (0.25% in saline) for 30 minutes and injected between two injections of fixative. After passing the period of fixation, the samples were dissected and arterial system in the Neck, Trunk and extremities were recognized. The degree of staining of the arteries categorized as low, medium and high. The data of staining of arteries were analyzed by chi square using SPSS 16.0 for Windows XP (SPSS Inc., Chicago, IL). P value< 0.05 was considered significant. In almost all of the experimental samples, axillary and femoral arteries were stained (P= 0.005). The arteries of the neck (carotids), the wall of thorax (Intercostals), arch of aorta, abdominal arteries (renal, mesenteric) and iliac arteries stained (p=0.000). This study presents inexpensive and readily used protocol for making easier learning and teaching of gross anatomy by differentiating arteries from their associated nerves and veins in cadavers.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.3 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300052en10.4067/S0717-95022012000300052
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Artery Cadaver Eosin Packed cells Staining
spellingShingle	Artery Cadaver Eosin Packed cells Staining Ghorbani,Rostam Tavakol,Zahra Ghanbari,Ali Contemporary Staining of Arterial System with Cadaver Fixation: An Experimental Study
description	Although many methods are investigated, cadavers have unique role for education of gross anatomy. This study was performed to make cadaveric based gross anatomy more enjoyable and decrease the time needed for achievement of arterial system for teachers and learners. The adult rabbits in control (n=3) and experimental groups (n=9) sacrificed following deep anesthesia. The samples were fixed by injection of fixative solution to the left ventricle and the corps dropped to the traditional fixative liquid for at least 1 month. The injected fixative solution of experimental group contained stained human red blood cells. The red blood cells stained by eosin (0.25% in saline) for 30 minutes and injected between two injections of fixative. After passing the period of fixation, the samples were dissected and arterial system in the Neck, Trunk and extremities were recognized. The degree of staining of the arteries categorized as low, medium and high. The data of staining of arteries were analyzed by chi square using SPSS 16.0 for Windows XP (SPSS Inc., Chicago, IL). P value< 0.05 was considered significant. In almost all of the experimental samples, axillary and femoral arteries were stained (P= 0.005). The arteries of the neck (carotids), the wall of thorax (Intercostals), arch of aorta, abdominal arteries (renal, mesenteric) and iliac arteries stained (p=0.000). This study presents inexpensive and readily used protocol for making easier learning and teaching of gross anatomy by differentiating arteries from their associated nerves and veins in cadavers.
author	Ghorbani,Rostam Tavakol,Zahra Ghanbari,Ali
author_facet	Ghorbani,Rostam Tavakol,Zahra Ghanbari,Ali
author_sort	Ghorbani,Rostam
title	Contemporary Staining of Arterial System with Cadaver Fixation: An Experimental Study
title_short	Contemporary Staining of Arterial System with Cadaver Fixation: An Experimental Study
title_full	Contemporary Staining of Arterial System with Cadaver Fixation: An Experimental Study
title_fullStr	Contemporary Staining of Arterial System with Cadaver Fixation: An Experimental Study
title_full_unstemmed	Contemporary Staining of Arterial System with Cadaver Fixation: An Experimental Study
title_sort	contemporary staining of arterial system with cadaver fixation: an experimental study
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300052
work_keys_str_mv	AT ghorbanirostam contemporarystainingofarterialsystemwithcadaverfixationanexperimentalstudy AT tavakolzahra contemporarystainingofarterialsystemwithcadaverfixationanexperimentalstudy AT ghanbariali contemporarystainingofarterialsystemwithcadaverfixationanexperimentalstudy
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Contemporary Staining of Arterial System with Cadaver Fixation: An Experimental Study

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