CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion

Many of Glycobyological aspects of human development remain still unclear, mainly in oral science, and this could be observed in a lack of literature with few and old papers about this subject. During tooth histo-morphogenesis changes occur in basement membrane composition, expression of signaling m...

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Autores principales:	Iglesias,Déborah Pitta Paraíso, Rego,Moacyr Jesus Barreto de Melo, Beltrão,Eduardo Isidoro Carneiro, Sobral,Ana Paula Veras
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Odontogenesis Carbohydrates Lectin histochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300056
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spelling	oai:scielo:S0717-950220120003000562013-02-20CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix SecretionIglesias,Déborah Pitta ParaísoRego,Moacyr Jesus Barreto de MeloBeltrão,Eduardo Isidoro CarneiroSobral,Ana Paula Veras Odontogenesis Carbohydrates Lectin histochemistry Many of Glycobyological aspects of human development remain still unclear, mainly in oral science, and this could be observed in a lack of literature with few and old papers about this subject. During tooth histo-morphogenesis changes occur in basement membrane composition, expression of signaling molecules and in localization of cell surface components, where glyco components could be involved. In this sense, this work aimed to analyze the ConA ligands commonly founded in glycoproteins cores and UEA-I ligands since fucolization is a Key event in many physiological and pathological processes. Therefore 15 jaws of human fetuses were ethically obtained, histologicaly processed and then submitted to lectin histochemistry with appropriated controls. The results showed that lectins staining increase their intensity during dental development; ConA only starts to recognize glucose/mannose residues on ectomesechymal cells in the crow phase revealing its ligands when the enamel matrix starts to be secreted. Interestingly, Con A ligands were not founded in the basement membrane of the stratum intermedium of the enamel differing from rodents models. The staining pattern of UEA-I was different, starting to be positive in the ectomesenchyma since the bud stage and shown variable expression in cell type and staining intensity, which appeared be directly proportional to the progress of odontogenesis. Thus, this work shows that Con A and UEA-I exhibit a growing staining directly proporcional to ameloblasts and odontoblasts cytodiferenciation and revels some glycan differences between human odontogenesis and rodents models.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.3 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300056en10.4067/S0717-95022012000300056
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Odontogenesis Carbohydrates Lectin histochemistry
spellingShingle	Odontogenesis Carbohydrates Lectin histochemistry Iglesias,Déborah Pitta Paraíso Rego,Moacyr Jesus Barreto de Melo Beltrão,Eduardo Isidoro Carneiro Sobral,Ana Paula Veras CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion
description	Many of Glycobyological aspects of human development remain still unclear, mainly in oral science, and this could be observed in a lack of literature with few and old papers about this subject. During tooth histo-morphogenesis changes occur in basement membrane composition, expression of signaling molecules and in localization of cell surface components, where glyco components could be involved. In this sense, this work aimed to analyze the ConA ligands commonly founded in glycoproteins cores and UEA-I ligands since fucolization is a Key event in many physiological and pathological processes. Therefore 15 jaws of human fetuses were ethically obtained, histologicaly processed and then submitted to lectin histochemistry with appropriated controls. The results showed that lectins staining increase their intensity during dental development; ConA only starts to recognize glucose/mannose residues on ectomesechymal cells in the crow phase revealing its ligands when the enamel matrix starts to be secreted. Interestingly, Con A ligands were not founded in the basement membrane of the stratum intermedium of the enamel differing from rodents models. The staining pattern of UEA-I was different, starting to be positive in the ectomesenchyma since the bud stage and shown variable expression in cell type and staining intensity, which appeared be directly proportional to the progress of odontogenesis. Thus, this work shows that Con A and UEA-I exhibit a growing staining directly proporcional to ameloblasts and odontoblasts cytodiferenciation and revels some glycan differences between human odontogenesis and rodents models.
author	Iglesias,Déborah Pitta Paraíso Rego,Moacyr Jesus Barreto de Melo Beltrão,Eduardo Isidoro Carneiro Sobral,Ana Paula Veras
author_facet	Iglesias,Déborah Pitta Paraíso Rego,Moacyr Jesus Barreto de Melo Beltrão,Eduardo Isidoro Carneiro Sobral,Ana Paula Veras
author_sort	Iglesias,Déborah Pitta Paraíso
title	CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion
title_short	CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion
title_full	CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion
title_fullStr	CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion
title_full_unstemmed	CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion
title_sort	cona and uea-i ligands during tooth development reveals differences between human and rodents models and shows the beginning of mineral extracelular matrix secretion
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300056
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CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion

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