Morphologic Alterations Resulting from Denervation of the Diaphragm in Rats

The morphological alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed...

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Autores principales:	Torrejais,M. M, Soares,J. C, Matheus,S. M. M, Francia-Farje,L. A. D, Mello,J. M, Vicente,E. J. D
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Diaphragm Muscle fiber Denervation Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300061
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spelling	oai:scielo:S0717-950220120003000612013-02-20Morphologic Alterations Resulting from Denervation of the Diaphragm in RatsTorrejais,M. MSoares,J. CMatheus,S. M. MFrancia-Farje,L. A. DMello,J. MVicente,E. J. D Diaphragm Muscle fiber Denervation Rat The morphological alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into two fragments, which were used for histological (H.E.) and histoenzymological (NADH-TR and myofibrillar ATPase). After 4 weeks, denervated muscle fibers showed important light microscopic alterations: atrophy with angular profiles in cross-sections, cytoplasm containing vacuoles, enlarged interstitial space with increased connective tissue, cellular infiltration, and muscle fibers without defined contours. The most marked alterations being observed for type IIb and IIa fibers. Eight and 12 weeks after denervation, the NADH-TR reaction showed that it was impossible to characterize the muscle fibers based on their metabolic profile.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.3 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300061en10.4067/S0717-95022012000300061
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Diaphragm Muscle fiber Denervation Rat
spellingShingle	Diaphragm Muscle fiber Denervation Rat Torrejais,M. M Soares,J. C Matheus,S. M. M Francia-Farje,L. A. D Mello,J. M Vicente,E. J. D Morphologic Alterations Resulting from Denervation of the Diaphragm in Rats
description	The morphological alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into two fragments, which were used for histological (H.E.) and histoenzymological (NADH-TR and myofibrillar ATPase). After 4 weeks, denervated muscle fibers showed important light microscopic alterations: atrophy with angular profiles in cross-sections, cytoplasm containing vacuoles, enlarged interstitial space with increased connective tissue, cellular infiltration, and muscle fibers without defined contours. The most marked alterations being observed for type IIb and IIa fibers. Eight and 12 weeks after denervation, the NADH-TR reaction showed that it was impossible to characterize the muscle fibers based on their metabolic profile.
author	Torrejais,M. M Soares,J. C Matheus,S. M. M Francia-Farje,L. A. D Mello,J. M Vicente,E. J. D
author_facet	Torrejais,M. M Soares,J. C Matheus,S. M. M Francia-Farje,L. A. D Mello,J. M Vicente,E. J. D
author_sort	Torrejais,M. M
title	Morphologic Alterations Resulting from Denervation of the Diaphragm in Rats
title_short	Morphologic Alterations Resulting from Denervation of the Diaphragm in Rats
title_full	Morphologic Alterations Resulting from Denervation of the Diaphragm in Rats
title_fullStr	Morphologic Alterations Resulting from Denervation of the Diaphragm in Rats
title_full_unstemmed	Morphologic Alterations Resulting from Denervation of the Diaphragm in Rats
title_sort	morphologic alterations resulting from denervation of the diaphragm in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300061
work_keys_str_mv	AT torrejaismm morphologicalterationsresultingfromdenervationofthediaphragminrats AT soaresjc morphologicalterationsresultingfromdenervationofthediaphragminrats AT matheussmm morphologicalterationsresultingfromdenervationofthediaphragminrats AT franciafarjelad morphologicalterationsresultingfromdenervationofthediaphragminrats AT mellojm morphologicalterationsresultingfromdenervationofthediaphragminrats AT vicenteejd morphologicalterationsresultingfromdenervationofthediaphragminrats
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Morphologic Alterations Resulting from Denervation of the Diaphragm in Rats

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