Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?

After careful observation of immuno-electron microscopic findings of the renal glomerular podocyte slit so far published by various authors, it should be noted that no convincing evidence is present for localization of nephrin-immunoreactivity in the slit itself, but that nephrin-immunoreactivity is...

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Autores principales:	Hipkaeo,Wiphawi, Suzuki,Ryoji, Abe,Hiroshi, Chaisiwamongkol,Kowit, Kondo,Hisatake
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Glomerulus Slit Nephrin Localization Immunohistochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400016
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spelling	oai:scielo:S0717-950220120004000162013-06-20Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?Hipkaeo,WiphawiSuzuki,RyojiAbe,HiroshiChaisiwamongkol,KowitKondo,Hisatake Glomerulus Slit Nephrin Localization Immunohistochemistry After careful observation of immuno-electron microscopic findings of the renal glomerular podocyte slit so far published by various authors, it should be noted that no convincing evidence is present for localization of nephrin-immunoreactivity in the slit itself, but that nephrin-immunoreactivity is dominantly localized along the membranes of podocyte pedicles from their tip over a substantial extension. In addition, the notion that the slit diaphragm is composed of strands is compatible with the widely distributed view that the slit strands represent rod-shaped nephrin molecules. However, 3-D findings that the diaphragm is composed of not only strands but also membranous elements have recently demonstrated separately by embedment-free section electron microscopy as well as by frozen/deep-etched replica electron microscopy. It is therefore suggested that the molecular composition of the slit diaphragm remain to be further elucidated, and that it is also necessary to re-consider how nephrin is involved in the renal glomerular filtration based on the exact localization along the podocyte membrane, but not in the slit itself. The present author would welcome any criticism and opinion on his consideration.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.4 20122012-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400016en10.4067/S0717-95022012000400016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Glomerulus Slit Nephrin Localization Immunohistochemistry
spellingShingle	Glomerulus Slit Nephrin Localization Immunohistochemistry Hipkaeo,Wiphawi Suzuki,Ryoji Abe,Hiroshi Chaisiwamongkol,Kowit Kondo,Hisatake Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?
description	After careful observation of immuno-electron microscopic findings of the renal glomerular podocyte slit so far published by various authors, it should be noted that no convincing evidence is present for localization of nephrin-immunoreactivity in the slit itself, but that nephrin-immunoreactivity is dominantly localized along the membranes of podocyte pedicles from their tip over a substantial extension. In addition, the notion that the slit diaphragm is composed of strands is compatible with the widely distributed view that the slit strands represent rod-shaped nephrin molecules. However, 3-D findings that the diaphragm is composed of not only strands but also membranous elements have recently demonstrated separately by embedment-free section electron microscopy as well as by frozen/deep-etched replica electron microscopy. It is therefore suggested that the molecular composition of the slit diaphragm remain to be further elucidated, and that it is also necessary to re-consider how nephrin is involved in the renal glomerular filtration based on the exact localization along the podocyte membrane, but not in the slit itself. The present author would welcome any criticism and opinion on his consideration.
author	Hipkaeo,Wiphawi Suzuki,Ryoji Abe,Hiroshi Chaisiwamongkol,Kowit Kondo,Hisatake
author_facet	Hipkaeo,Wiphawi Suzuki,Ryoji Abe,Hiroshi Chaisiwamongkol,Kowit Kondo,Hisatake
author_sort	Hipkaeo,Wiphawi
title	Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?
title_short	Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?
title_full	Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?
title_fullStr	Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?
title_full_unstemmed	Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?
title_sort	commentary: is the evidence for localization of nephrin on the slit diaphragm rigid?
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400016
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Commentary: Is the Evidence for Localization of Nephrin on the Slit Diaphragm Rigid?

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