Malathion Affects Spermatogenic Proliferation in Mouse

The restriction of the mechanisms of cell proliferation in murine seminiferous epithelium, in terms of induction of programmed cell death until recently has not been fully analyzed. The aim of this work was to assess the effect of Malathion (MP) on testicular morphology and function in mouse spermat...

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Autores principales:	Penna-Videau,S, Bustos-Obregón,E, Cermeño-Vivas,J. R, Chirino,D
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Malathion Testis mouse Apoptosis Morphology p53 Proliferation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400023
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spelling	oai:scielo:S0717-950220120004000232013-06-20Malathion Affects Spermatogenic Proliferation in MousePenna-Videau,SBustos-Obregón,ECermeño-Vivas,J. RChirino,D Malathion Testis mouse Apoptosis Morphology p53 Proliferation The restriction of the mechanisms of cell proliferation in murine seminiferous epithelium, in terms of induction of programmed cell death until recently has not been fully analyzed. The aim of this work was to assess the effect of Malathion (MP) on testicular morphology and function in mouse spermatogenesis. For the experiments, male albino mice of strain NMRI-IVIC, weighing between 30-40 g were used, and divided into control and experimental groups of 5 each. The animals of the experimental groups were injected with a single dose of MP: 241mg/kg weight (1/12 LD 50 ) resuspended in 0.9% saline, intraperitoneally. Animals were sacrificed at 8.3, 16.6 and 33.2 days post-injection (first, second and third spermatogenic cycles). Testicular samples were obtained for light microscopy (LM), transmission electron microscopy procedures, and to detect apoptosis and p53 antigen by immunohistochemical methods. Blood was collected to quantify testosterone and plasmatic cholinesterase activity. From 8.3 days, Sertoli cell vacuolization, karyolisis of pachytene spermatocytes and Leydig cells and a decreased in average of the diameter of seminiferous tubules was observed. No damage to inter-Sertoli cells junctions was detected. Percentage of seminiferous tubules showing germ cells apoptosis was increased from 8.3 days, plasmatic acetylcholinesterase activity was reduced in the group treated only 24 hours after administration of MP. Serum testosterone levels were low in treated animals at 16. 6 and 33.2 days. p53 was mostly expressed in pachytene spermatocytes from 8d. The findings of this study indicate that MP alters the testicular function affecting the DNA and interfering with spermatogenesis as well as steroidogenesis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.4 20122012-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400023en10.4067/S0717-95022012000400023
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Malathion Testis mouse Apoptosis Morphology p53 Proliferation
spellingShingle	Malathion Testis mouse Apoptosis Morphology p53 Proliferation Penna-Videau,S Bustos-Obregón,E Cermeño-Vivas,J. R Chirino,D Malathion Affects Spermatogenic Proliferation in Mouse
description	The restriction of the mechanisms of cell proliferation in murine seminiferous epithelium, in terms of induction of programmed cell death until recently has not been fully analyzed. The aim of this work was to assess the effect of Malathion (MP) on testicular morphology and function in mouse spermatogenesis. For the experiments, male albino mice of strain NMRI-IVIC, weighing between 30-40 g were used, and divided into control and experimental groups of 5 each. The animals of the experimental groups were injected with a single dose of MP: 241mg/kg weight (1/12 LD 50 ) resuspended in 0.9% saline, intraperitoneally. Animals were sacrificed at 8.3, 16.6 and 33.2 days post-injection (first, second and third spermatogenic cycles). Testicular samples were obtained for light microscopy (LM), transmission electron microscopy procedures, and to detect apoptosis and p53 antigen by immunohistochemical methods. Blood was collected to quantify testosterone and plasmatic cholinesterase activity. From 8.3 days, Sertoli cell vacuolization, karyolisis of pachytene spermatocytes and Leydig cells and a decreased in average of the diameter of seminiferous tubules was observed. No damage to inter-Sertoli cells junctions was detected. Percentage of seminiferous tubules showing germ cells apoptosis was increased from 8.3 days, plasmatic acetylcholinesterase activity was reduced in the group treated only 24 hours after administration of MP. Serum testosterone levels were low in treated animals at 16. 6 and 33.2 days. p53 was mostly expressed in pachytene spermatocytes from 8d. The findings of this study indicate that MP alters the testicular function affecting the DNA and interfering with spermatogenesis as well as steroidogenesis.
author	Penna-Videau,S Bustos-Obregón,E Cermeño-Vivas,J. R Chirino,D
author_facet	Penna-Videau,S Bustos-Obregón,E Cermeño-Vivas,J. R Chirino,D
author_sort	Penna-Videau,S
title	Malathion Affects Spermatogenic Proliferation in Mouse
title_short	Malathion Affects Spermatogenic Proliferation in Mouse
title_full	Malathion Affects Spermatogenic Proliferation in Mouse
title_fullStr	Malathion Affects Spermatogenic Proliferation in Mouse
title_full_unstemmed	Malathion Affects Spermatogenic Proliferation in Mouse
title_sort	malathion affects spermatogenic proliferation in mouse
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400023
work_keys_str_mv	AT pennavideaus malathionaffectsspermatogenicproliferationinmouse AT bustosobregone malathionaffectsspermatogenicproliferationinmouse AT cermenovivasjr malathionaffectsspermatogenicproliferationinmouse AT chirinod malathionaffectsspermatogenicproliferationinmouse
_version_	1718444802988572672

Malathion Affects Spermatogenic Proliferation in Mouse

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