The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma

This study aimed to observe the regularity of liver cell apoptosis and expression of apoptosis related gene Bcl-2 and Bax in the induced cancer, and explore the relationship between apoptosis and the development of liver cancer. In this study, 84 rats were used, 72 rats as experimental group induced...

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Autores principales:	Hongfu,Liu, Luping,Zhang, Shaojie,Chen, Zengxian,Wang, Fei,Huang, Dong,Wang
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Primary hepatic carcinoma Apoptosis Bcl-2 Bax Rats
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400032
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spelling	oai:scielo:S0717-950220120004000322013-06-20The Role and Significance of Bcl-2 and Bax in the Hepatic CarcinomaHongfu,LiuLuping,ZhangShaojie,ChenZengxian,WangFei,HuangDong,Wang Primary hepatic carcinoma Apoptosis Bcl-2 Bax Rats This study aimed to observe the regularity of liver cell apoptosis and expression of apoptosis related gene Bcl-2 and Bax in the induced cancer, and explore the relationship between apoptosis and the development of liver cancer. In this study, 84 rats were used, 72 rats as experimental group induced by drinking water containing DEN, 12 rats as control group (CG). After laparotomy, the form, color, texture of the liver and metastatic tumor in both control and experimental groups were observed and recorded. The metastatic tumor and the liver tissue were sectioned and stained with hematoxylin-eosin staining to demonstrate the characteristic in morphological changes, the Hoechst 33342 fluorescent staining was applied to show and count the rate of apoptotic cell, and the in situ hybridization technique was used to detect the Bcl-2 and Bax expression. The results showed that the process of carcinogenesis can be divided into three periods: hepatic toxic lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis. The Hoechst 33342 fluorescent staining showed that the rate of apoptosis in the CG, hepatic toxic lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis were 6.7%,18.8%,17.4% and 51.2% c2=33.62, P<0.05 respectively. In situ hybridization showed the yellow positive reaction products of Bcl-2 and Bax located in the nucleus and cytoplasm of cell in diffusive distribution. The average optical densities of Bcl-2 and Bax expression were 0.1697±0.0101 and 0.1383±0.0093 in the control group, 0.1431±0.0072 and 0.1523±0.0103 in hepatic toxic lesion, 0.1261±0.0164 and 0.1639±0.0133 in hepatic proliferation/cirrhosis, 0.1034± 0.0124 and 0.1785±0.0191 in hepatic carcinogenesis, indicating that the expression of Bcl-2 decreased, but that of Bax increased with the development of liver cancer in experimental groups. In DEN induced liver cancer, the origination and development of liver cancer were accompanied with proliferation and abnormal apoptosis, which was related to the abnormal expression of gene Bcl-2 and Bax.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.4 20122012-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400032en10.4067/S0717-95022012000400032
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Primary hepatic carcinoma Apoptosis Bcl-2 Bax Rats
spellingShingle	Primary hepatic carcinoma Apoptosis Bcl-2 Bax Rats Hongfu,Liu Luping,Zhang Shaojie,Chen Zengxian,Wang Fei,Huang Dong,Wang The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma
description	This study aimed to observe the regularity of liver cell apoptosis and expression of apoptosis related gene Bcl-2 and Bax in the induced cancer, and explore the relationship between apoptosis and the development of liver cancer. In this study, 84 rats were used, 72 rats as experimental group induced by drinking water containing DEN, 12 rats as control group (CG). After laparotomy, the form, color, texture of the liver and metastatic tumor in both control and experimental groups were observed and recorded. The metastatic tumor and the liver tissue were sectioned and stained with hematoxylin-eosin staining to demonstrate the characteristic in morphological changes, the Hoechst 33342 fluorescent staining was applied to show and count the rate of apoptotic cell, and the in situ hybridization technique was used to detect the Bcl-2 and Bax expression. The results showed that the process of carcinogenesis can be divided into three periods: hepatic toxic lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis. The Hoechst 33342 fluorescent staining showed that the rate of apoptosis in the CG, hepatic toxic lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis were 6.7%,18.8%,17.4% and 51.2% c2=33.62, P<0.05 respectively. In situ hybridization showed the yellow positive reaction products of Bcl-2 and Bax located in the nucleus and cytoplasm of cell in diffusive distribution. The average optical densities of Bcl-2 and Bax expression were 0.1697±0.0101 and 0.1383±0.0093 in the control group, 0.1431±0.0072 and 0.1523±0.0103 in hepatic toxic lesion, 0.1261±0.0164 and 0.1639±0.0133 in hepatic proliferation/cirrhosis, 0.1034± 0.0124 and 0.1785±0.0191 in hepatic carcinogenesis, indicating that the expression of Bcl-2 decreased, but that of Bax increased with the development of liver cancer in experimental groups. In DEN induced liver cancer, the origination and development of liver cancer were accompanied with proliferation and abnormal apoptosis, which was related to the abnormal expression of gene Bcl-2 and Bax.
author	Hongfu,Liu Luping,Zhang Shaojie,Chen Zengxian,Wang Fei,Huang Dong,Wang
author_facet	Hongfu,Liu Luping,Zhang Shaojie,Chen Zengxian,Wang Fei,Huang Dong,Wang
author_sort	Hongfu,Liu
title	The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma
title_short	The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma
title_full	The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma
title_fullStr	The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma
title_full_unstemmed	The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma
title_sort	role and significance of bcl-2 and bax in the hepatic carcinoma
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400032
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The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma

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