Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements

The aim was to examine the morphology of spermatozoa with different staining methods and aimed to find the better staining methods for morphology of spermatozoa in our study. Randomized 67 patients taken for the study who were admitted to Assisted Reproductive Techniques Unit. In the first part of t...

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Autores principales:	Aksoy,Emine, Aktan,Tahsin Murad, Duman,Selcuk, Cuce,Gokhan
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Spermatozoa Morphology Histological stains
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400045
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spelling	oai:scielo:S0717-950220120004000452013-06-20Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric MeasurementsAksoy,EmineAktan,Tahsin MuradDuman,SelcukCuce,Gokhan Spermatozoa Morphology Histological stains The aim was to examine the morphology of spermatozoa with different staining methods and aimed to find the better staining methods for morphology of spermatozoa in our study. Randomized 67 patients taken for the study who were admitted to Assisted Reproductive Techniques Unit. In the first part of the study, smears were stained with Hematoxylin Eosin (HE), Toluidin Blue (TB), Giemsa, Wright, ferrous Weigert haematoxylin stain, Orange G, eosin-aniline blue dye, Shorr Method, Papanicolau, Berg Method, Light Green stain, Acridine Orange (AO) and Janus Green dyes. In the second part of the study, smear preparations of 10 patients with normozoospermic were stained with HE, Toluidin Blue (TB), Shorr Method and Papanicolau. Four measurements were made including the middle piece, head length- head width and tail length for 200 spermatozoa with normal morphology. Comparisons were made between the stains that which showed a better morphology. Condensation assessment was not possible in smears stained with Shorr, Berg, Method, AO. Better assessment of condensation could be made in other stains. In the second part the smallestvalues belonged to of TB stain according to measurements of head of the spermatozoa. There was a significant difference at the head length with TB stain. Although measurements of Shorr and Papanicolau areclose to each otherand the largestvalues belonged to Papanicolau dye. It was concluded that measurement values in human sperm morphology could alter with the used staining method.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.4 20122012-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400045en10.4067/S0717-95022012000400045
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Spermatozoa Morphology Histological stains
spellingShingle	Spermatozoa Morphology Histological stains Aksoy,Emine Aktan,Tahsin Murad Duman,Selcuk Cuce,Gokhan Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements
description	The aim was to examine the morphology of spermatozoa with different staining methods and aimed to find the better staining methods for morphology of spermatozoa in our study. Randomized 67 patients taken for the study who were admitted to Assisted Reproductive Techniques Unit. In the first part of the study, smears were stained with Hematoxylin Eosin (HE), Toluidin Blue (TB), Giemsa, Wright, ferrous Weigert haematoxylin stain, Orange G, eosin-aniline blue dye, Shorr Method, Papanicolau, Berg Method, Light Green stain, Acridine Orange (AO) and Janus Green dyes. In the second part of the study, smear preparations of 10 patients with normozoospermic were stained with HE, Toluidin Blue (TB), Shorr Method and Papanicolau. Four measurements were made including the middle piece, head length- head width and tail length for 200 spermatozoa with normal morphology. Comparisons were made between the stains that which showed a better morphology. Condensation assessment was not possible in smears stained with Shorr, Berg, Method, AO. Better assessment of condensation could be made in other stains. In the second part the smallestvalues belonged to of TB stain according to measurements of head of the spermatozoa. There was a significant difference at the head length with TB stain. Although measurements of Shorr and Papanicolau areclose to each otherand the largestvalues belonged to Papanicolau dye. It was concluded that measurement values in human sperm morphology could alter with the used staining method.
author	Aksoy,Emine Aktan,Tahsin Murad Duman,Selcuk Cuce,Gokhan
author_facet	Aksoy,Emine Aktan,Tahsin Murad Duman,Selcuk Cuce,Gokhan
author_sort	Aksoy,Emine
title	Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements
title_short	Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements
title_full	Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements
title_fullStr	Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements
title_full_unstemmed	Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements
title_sort	assessment of spermatozoa morphology under light microscopy with different histologic stains and comparison of morphometric measurements
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400045
work_keys_str_mv	AT aksoyemine assessmentofspermatozoamorphologyunderlightmicroscopywithdifferenthistologicstainsandcomparisonofmorphometricmeasurements AT aktantahsinmurad assessmentofspermatozoamorphologyunderlightmicroscopywithdifferenthistologicstainsandcomparisonofmorphometricmeasurements AT dumanselcuk assessmentofspermatozoamorphologyunderlightmicroscopywithdifferenthistologicstainsandcomparisonofmorphometricmeasurements AT cucegokhan assessmentofspermatozoamorphologyunderlightmicroscopywithdifferenthistologicstainsandcomparisonofmorphometricmeasurements
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Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements

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