Quantitative Morphology Update: Image Analysis

Quantitative Morphology Update: Image Analysis

Quantitative morphology is a reliable tool in developmental, clinical and aging biology. Quantitative data of histological improvement and/or impairment due to dietary manipulations or pharmacological interventions draws the attention of countless researchers worldwide. Morphometry allows a wide ran...

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Autores principales: Fernandes-Santos,Caroline, Souza-Mello,Vanessa, Faria,Tatiane da Silva, Mandarim-de-Lacerda,Carlos Alberto
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2013
Materias:
Quantitative morphology
Image analysis
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100003
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spelling oai:scielo:S0717-950220130001000032014-09-09Quantitative Morphology Update: Image AnalysisFernandes-Santos,CarolineSouza-Mello,VanessaFaria,Tatiane da SilvaMandarim-de-Lacerda,Carlos Alberto Quantitative morphology Image analysis Quantitative morphology is a reliable tool in developmental, clinical and aging biology. Quantitative data of histological improvement and/or impairment due to dietary manipulations or pharmacological interventions draws the attention of countless researchers worldwide. Morphometry allows a wide range of two-dimension analysis to be performed, whereas image segmentation allows the measurement of a single structure or the determination of the intensity of its color after adequate immunostaining or even the area occupied by a specific sort of cell. When it comes to unbiased analysis, consideration should be given to suitable tissue perfusion, fixation and embedding, avoiding artifacts. Furthermore, tissue shrinkage should be taken into account. In order to ensure reproducibility, one of the most important principles in scientific research, and to allow different groups to be compared, image acquisition and image segmentation emerge as crucial steps and should be standardized. This paper aims to approach some important care for appropriate tissue processing and evaluation, and shows some practical examples of how morphometry and image segmentation can be applied to your experiment.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.1 20132013-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100003en10.4067/S0717-95022013000100003
institution Scielo Chile
collection Scielo Chile
language English
topic Quantitative morphology
Image analysis
spellingShingle Quantitative morphology
Image analysis
Fernandes-Santos,Caroline
Souza-Mello,Vanessa
Faria,Tatiane da Silva
Mandarim-de-Lacerda,Carlos Alberto
Quantitative Morphology Update: Image Analysis
description Quantitative morphology is a reliable tool in developmental, clinical and aging biology. Quantitative data of histological improvement and/or impairment due to dietary manipulations or pharmacological interventions draws the attention of countless researchers worldwide. Morphometry allows a wide range of two-dimension analysis to be performed, whereas image segmentation allows the measurement of a single structure or the determination of the intensity of its color after adequate immunostaining or even the area occupied by a specific sort of cell. When it comes to unbiased analysis, consideration should be given to suitable tissue perfusion, fixation and embedding, avoiding artifacts. Furthermore, tissue shrinkage should be taken into account. In order to ensure reproducibility, one of the most important principles in scientific research, and to allow different groups to be compared, image acquisition and image segmentation emerge as crucial steps and should be standardized. This paper aims to approach some important care for appropriate tissue processing and evaluation, and shows some practical examples of how morphometry and image segmentation can be applied to your experiment.
author Fernandes-Santos,Caroline
Souza-Mello,Vanessa
Faria,Tatiane da Silva
Mandarim-de-Lacerda,Carlos Alberto
author_facet Fernandes-Santos,Caroline
Souza-Mello,Vanessa
Faria,Tatiane da Silva
Mandarim-de-Lacerda,Carlos Alberto
author_sort Fernandes-Santos,Caroline
title Quantitative Morphology Update: Image Analysis
title_short Quantitative Morphology Update: Image Analysis
title_full Quantitative Morphology Update: Image Analysis
title_fullStr Quantitative Morphology Update: Image Analysis
title_full_unstemmed Quantitative Morphology Update: Image Analysis
title_sort quantitative morphology update: image analysis
publisher Sociedad Chilena de Anatomía
publishDate 2013
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100003
work_keys_str_mv AT fernandessantoscaroline quantitativemorphologyupdateimageanalysis
AT souzamellovanessa quantitativemorphologyupdateimageanalysis
AT fariatatianedasilva quantitativemorphologyupdateimageanalysis
AT mandarimdelacerdacarlosalberto quantitativemorphologyupdateimageanalysis
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