Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue

The aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different man...

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Autores principales:	Duman,Selcuk, Aktan,Tahsin Murad, Cuce,Gokhan, Cihantimur,Bulent, Tokac,Mehmet, Akbulut,Habip
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	Adipose tissue Stem cell Angiogenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100009
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spelling	oai:scielo:S0717-950220130001000092014-09-09Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose TissueDuman,SelcukAktan,Tahsin MuradCuce,GokhanCihantimur,BulentTokac,MehmetAkbulut,Habip Adipose tissue Stem cell Angiogenesis The aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fundamental stepin the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural properties after with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique. Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a micro filter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. These slides were stained with H&E and Toluidine Blue. Paraffin sections were immunohistochemically stained with CD34, von Willebrand Factor, CD73, CD90 and CD105. Smear preparations showed a continuous three dimensional plasma membrane appearance of adipocytes. C+ and C- showed expression of CD34, von Willebrand Factor, CD73, CD90, CD105. C+ seems to have more free cells expressing than C-. While passing the filter of Lipokit, large adipocytes and connective tissue parts disintegrate and thus increases the surface area of lipoaspirate. Lipokit® machine release the group cells which are necessary for angiogenesis and they become more freely to construct angiogenesis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.1 20132013-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100009en10.4067/S0717-95022013000100009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adipose tissue Stem cell Angiogenesis
spellingShingle	Adipose tissue Stem cell Angiogenesis Duman,Selcuk Aktan,Tahsin Murad Cuce,Gokhan Cihantimur,Bulent Tokac,Mehmet Akbulut,Habip Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue
description	The aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fundamental stepin the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural properties after with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique. Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a micro filter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. These slides were stained with H&E and Toluidine Blue. Paraffin sections were immunohistochemically stained with CD34, von Willebrand Factor, CD73, CD90 and CD105. Smear preparations showed a continuous three dimensional plasma membrane appearance of adipocytes. C+ and C- showed expression of CD34, von Willebrand Factor, CD73, CD90, CD105. C+ seems to have more free cells expressing than C-. While passing the filter of Lipokit, large adipocytes and connective tissue parts disintegrate and thus increases the surface area of lipoaspirate. Lipokit® machine release the group cells which are necessary for angiogenesis and they become more freely to construct angiogenesis.
author	Duman,Selcuk Aktan,Tahsin Murad Cuce,Gokhan Cihantimur,Bulent Tokac,Mehmet Akbulut,Habip
author_facet	Duman,Selcuk Aktan,Tahsin Murad Cuce,Gokhan Cihantimur,Bulent Tokac,Mehmet Akbulut,Habip
author_sort	Duman,Selcuk
title	Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue
title_short	Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue
title_full	Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue
title_fullStr	Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue
title_full_unstemmed	Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue
title_sort	effects of lipokit® centrifugation on morphology and resident cells of adipose tissue
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100009
work_keys_str_mv	AT dumanselcuk effectsoflipokitcentrifugationonmorphologyandresidentcellsofadiposetissue AT aktantahsinmurad effectsoflipokitcentrifugationonmorphologyandresidentcellsofadiposetissue AT cucegokhan effectsoflipokitcentrifugationonmorphologyandresidentcellsofadiposetissue AT cihantimurbulent effectsoflipokitcentrifugationonmorphologyandresidentcellsofadiposetissue AT tokacmehmet effectsoflipokitcentrifugationonmorphologyandresidentcellsofadiposetissue AT akbuluthabip effectsoflipokitcentrifugationonmorphologyandresidentcellsofadiposetissue
_version_	1718444810072752129

Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue

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