Effects of Zinc During Lactation on the Junctional Epithelium and Inserted Gum in Rats

The purpose of this study was to evaluate the effects of zinc, during lactation, on the junctional epithelium and inserted gum of the first upper molar of rats. The study used one-day old male rats, divided into two groups: those whose mother had been treated with 300 mg zinc chloride (ZnCl2) in the...

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Autores principales: Felippini,Ana Luiza de Carvalho, Sala,Miguel Angel, Lopes,Ruberval Armando, Silva,Gilberto André e
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2013
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100024
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spelling oai:scielo:S0717-950220130001000242014-09-09Effects of Zinc During Lactation on the Junctional Epithelium and Inserted Gum in RatsFelippini,Ana Luiza de CarvalhoSala,Miguel AngelLopes,Ruberval ArmandoSilva,Gilberto André e Zinc Lactation Junctional epithelium Inserted gum The purpose of this study was to evaluate the effects of zinc, during lactation, on the junctional epithelium and inserted gum of the first upper molar of rats. The study used one-day old male rats, divided into two groups: those whose mother had been treated with 300 mg zinc chloride (ZnCl2) in the drinker water (treated group), and those whose mothers did not receive ZnCl2 (control group). After 21 days, the rat pups were sacrificed. Using karyometrical techniques, the greater (D) and smaller (d) nuclear diameters of the different layers of the junctional and inserted gum epithelia were determined, and the mean geometric diameter, D/d ratio, perimeter, area, volume, volume/area ratio, eccentricity, shape coefficient, and the contour index were estimated. The 100-point Merz grid was used with the purpose of evaluating the citoplasmatic and celular volume, the nucleus/citoplasm relationship, number density, outer surface/basal layer ratio, the thickness of epithelial layers, and the surface density. The results were submitted to statistical analysis using the Wilcoxon-Mann-Whitney test. The nuclei of the studied structures were significantly smaller, and the stereological results demonstrated that there were smaller cells, hence meaning a greater number of cells per mm3 of tissue, in the treated group. Zinc caused changes on the studied epitheliums, according to morphometric and stereological evaluations.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.1 20132013-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100024en10.4067/S0717-95022013000100024
institution Scielo Chile
collection Scielo Chile
language English
topic Zinc
Lactation
Junctional epithelium
Inserted gum
spellingShingle Zinc
Lactation
Junctional epithelium
Inserted gum
Felippini,Ana Luiza de Carvalho
Sala,Miguel Angel
Lopes,Ruberval Armando
Silva,Gilberto André e
Effects of Zinc During Lactation on the Junctional Epithelium and Inserted Gum in Rats
description The purpose of this study was to evaluate the effects of zinc, during lactation, on the junctional epithelium and inserted gum of the first upper molar of rats. The study used one-day old male rats, divided into two groups: those whose mother had been treated with 300 mg zinc chloride (ZnCl2) in the drinker water (treated group), and those whose mothers did not receive ZnCl2 (control group). After 21 days, the rat pups were sacrificed. Using karyometrical techniques, the greater (D) and smaller (d) nuclear diameters of the different layers of the junctional and inserted gum epithelia were determined, and the mean geometric diameter, D/d ratio, perimeter, area, volume, volume/area ratio, eccentricity, shape coefficient, and the contour index were estimated. The 100-point Merz grid was used with the purpose of evaluating the citoplasmatic and celular volume, the nucleus/citoplasm relationship, number density, outer surface/basal layer ratio, the thickness of epithelial layers, and the surface density. The results were submitted to statistical analysis using the Wilcoxon-Mann-Whitney test. The nuclei of the studied structures were significantly smaller, and the stereological results demonstrated that there were smaller cells, hence meaning a greater number of cells per mm3 of tissue, in the treated group. Zinc caused changes on the studied epitheliums, according to morphometric and stereological evaluations.
author Felippini,Ana Luiza de Carvalho
Sala,Miguel Angel
Lopes,Ruberval Armando
Silva,Gilberto André e
author_facet Felippini,Ana Luiza de Carvalho
Sala,Miguel Angel
Lopes,Ruberval Armando
Silva,Gilberto André e
author_sort Felippini,Ana Luiza de Carvalho
title Effects of Zinc During Lactation on the Junctional Epithelium and Inserted Gum in Rats
title_short Effects of Zinc During Lactation on the Junctional Epithelium and Inserted Gum in Rats
title_full Effects of Zinc During Lactation on the Junctional Epithelium and Inserted Gum in Rats
title_fullStr Effects of Zinc During Lactation on the Junctional Epithelium and Inserted Gum in Rats
title_full_unstemmed Effects of Zinc During Lactation on the Junctional Epithelium and Inserted Gum in Rats
title_sort effects of zinc during lactation on the junctional epithelium and inserted gum in rats
publisher Sociedad Chilena de Anatomía
publishDate 2013
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100024
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AT lopesrubervalarmando effectsofzincduringlactationonthejunctionalepitheliumandinsertedguminrats
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