Morphological Study of Bone Cranial in Athymic Mice

The aim of our research was to create an osteogenic unit in the skulls of athymic mice; however, the first challenge we faced was to find sufficient and adequate data that would allow us to determine the morphological, immunohistochemical and microtopographical characteristics that could be used as...

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Autores principales:	Sesman,Ana, Ruvalcaba,Erika, Herrera,Arturo, Sánchez Guerrero,Sergio, Lecona,Hugo, Baena-Ocampo,Leticia, Solis,Lilia, Ávila,Hector, García de la Puente,Silvestre, Vargas,Betha, Guerrero,Xochitl, Velasquillo,Cristina
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	Osteogenesis Osteoinduction Osteoconductivity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100050
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spelling	oai:scielo:S0717-950220130001000502014-09-09Morphological Study of Bone Cranial in Athymic MiceSesman,AnaRuvalcaba,ErikaHerrera,ArturoSánchez Guerrero,SergioLecona,HugoBaena-Ocampo,LeticiaSolis,LiliaÁvila,HectorGarcía de la Puente,SilvestreVargas,BethaGuerrero,XochitlVelasquillo,Cristina Osteogenesis Osteoinduction Osteoconductivity The aim of our research was to create an osteogenic unit in the skulls of athymic mice; however, the first challenge we faced was to find sufficient and adequate data that would allow us to determine the morphological, immunohistochemical and microtopographical characteristics that could be used as normality standards in athymic mice skulls and, hence, a reference in the event of achieving the formation of de novo bone using the osteogenic unit we proposed. Knowing the normal bone morphology in the skull of athymic mice was a necessary precondition to develop subsequently an osteogenic unit possessing the Osteogenesis, Osteoinduction and Osteoconductivity that could be compared versus those in the normal bone during its formations and remodeling processes. Therefore, we conducted a pilot study to determine bone morphological characteristics in the skull of athymic mice by means of specific histological staining: hematoxylin-eosin and Von Kossa, for osteoid tissue and mineralized bone, and Masson Tri-chrome for ossified areas. We also use immunohistochemistry to detect bone formation markers: alkaline phosphatase resulting from osteoblastic activity stimulation, type 1 collagen a bonematrix structural protein; Osteopontine, a protein specifically synthesized by osteoblasts that favors cell proliferation and remodeling in bone defects; Osteocalcine, a peptide hormone produced by osteoblasts during bone formation; and, Runx 2, a transcription factor expressed by stem cells which stimulates bone differentiation. Likewise, we used electron microscopy on the newly formed tissue to determine the presence of organic deposits, such as calcium, phosphate and magnesium in bone tissue.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.1 20132013-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100050en10.4067/S0717-95022013000100050
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Osteogenesis Osteoinduction Osteoconductivity
spellingShingle	Osteogenesis Osteoinduction Osteoconductivity Sesman,Ana Ruvalcaba,Erika Herrera,Arturo Sánchez Guerrero,Sergio Lecona,Hugo Baena-Ocampo,Leticia Solis,Lilia Ávila,Hector García de la Puente,Silvestre Vargas,Betha Guerrero,Xochitl Velasquillo,Cristina Morphological Study of Bone Cranial in Athymic Mice
description	The aim of our research was to create an osteogenic unit in the skulls of athymic mice; however, the first challenge we faced was to find sufficient and adequate data that would allow us to determine the morphological, immunohistochemical and microtopographical characteristics that could be used as normality standards in athymic mice skulls and, hence, a reference in the event of achieving the formation of de novo bone using the osteogenic unit we proposed. Knowing the normal bone morphology in the skull of athymic mice was a necessary precondition to develop subsequently an osteogenic unit possessing the Osteogenesis, Osteoinduction and Osteoconductivity that could be compared versus those in the normal bone during its formations and remodeling processes. Therefore, we conducted a pilot study to determine bone morphological characteristics in the skull of athymic mice by means of specific histological staining: hematoxylin-eosin and Von Kossa, for osteoid tissue and mineralized bone, and Masson Tri-chrome for ossified areas. We also use immunohistochemistry to detect bone formation markers: alkaline phosphatase resulting from osteoblastic activity stimulation, type 1 collagen a bonematrix structural protein; Osteopontine, a protein specifically synthesized by osteoblasts that favors cell proliferation and remodeling in bone defects; Osteocalcine, a peptide hormone produced by osteoblasts during bone formation; and, Runx 2, a transcription factor expressed by stem cells which stimulates bone differentiation. Likewise, we used electron microscopy on the newly formed tissue to determine the presence of organic deposits, such as calcium, phosphate and magnesium in bone tissue.
author	Sesman,Ana Ruvalcaba,Erika Herrera,Arturo Sánchez Guerrero,Sergio Lecona,Hugo Baena-Ocampo,Leticia Solis,Lilia Ávila,Hector García de la Puente,Silvestre Vargas,Betha Guerrero,Xochitl Velasquillo,Cristina
author_facet	Sesman,Ana Ruvalcaba,Erika Herrera,Arturo Sánchez Guerrero,Sergio Lecona,Hugo Baena-Ocampo,Leticia Solis,Lilia Ávila,Hector García de la Puente,Silvestre Vargas,Betha Guerrero,Xochitl Velasquillo,Cristina
author_sort	Sesman,Ana
title	Morphological Study of Bone Cranial in Athymic Mice
title_short	Morphological Study of Bone Cranial in Athymic Mice
title_full	Morphological Study of Bone Cranial in Athymic Mice
title_fullStr	Morphological Study of Bone Cranial in Athymic Mice
title_full_unstemmed	Morphological Study of Bone Cranial in Athymic Mice
title_sort	morphological study of bone cranial in athymic mice
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100050
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Morphological Study of Bone Cranial in Athymic Mice

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