Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human Embryos

The role of bone morphogenetic proteins (BMP-s) in the development of the nervous system has been widely studied on avian and rodent embryos. Human embryos have rarely been available for detection of BMP expression. In this study 39 human embryos of Carnegie stages (CS) 10-20 were investigated. The...

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Autores principales:	Namm,Aimar, Arend,Andres, Aunapuu,Marina
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	Neural tube Human embryo BMP-2 BMP-4 Immunohistochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200018
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spelling	oai:scielo:S0717-950220130002000182014-09-09Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human EmbryosNamm,AimarArend,AndresAunapuu,Marina Neural tube Human embryo BMP-2 BMP-4 Immunohistochemistry The role of bone morphogenetic proteins (BMP-s) in the development of the nervous system has been widely studied on avian and rodent embryos. Human embryos have rarely been available for detection of BMP expression. In this study 39 human embryos of Carnegie stages (CS) 10-20 were investigated. The embryos were fixed in paraformaldehyde, embedded in paraffin and sectioned serially in transverse direction. BMP-2 and BMP-4 protein expression in the developing neural tube and the caudal spinal cord was determined by immunohistochemistry. Our data show that BMP-s tend to be more expressed in the neural tube in earlier stages; in particular, BMP-4 staining was found to be higher at CS10 compared to CS20. More detailed analysis was performed on embryos of CS14-18. Stronger BMP-2 and BMP-4 expression was found in the dorsal part than in the ventral part of the spinal cord. No differences were seen in the staining intensity of BMP-s in the spinal ganglia. Interestingly, in neural crest cells BMP-2 staining was stronger at CS16 and CS18 as compared to CS14, while no differences were found in BMP-4 staining. On the other hand, in the non-neural ectoderm BMP-4 staining was found to be stronger at CS16 than at CS14, while no differences were seen for BMP-2. In conclusion, expression of BMP-s in the developing neural tube and spinal cord of human embryos is generally in accordance with the findings made in rodents and birds.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.2 20132013-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200018en10.4067/S0717-95022013000200018
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Neural tube Human embryo BMP-2 BMP-4 Immunohistochemistry
spellingShingle	Neural tube Human embryo BMP-2 BMP-4 Immunohistochemistry Namm,Aimar Arend,Andres Aunapuu,Marina Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human Embryos
description	The role of bone morphogenetic proteins (BMP-s) in the development of the nervous system has been widely studied on avian and rodent embryos. Human embryos have rarely been available for detection of BMP expression. In this study 39 human embryos of Carnegie stages (CS) 10-20 were investigated. The embryos were fixed in paraformaldehyde, embedded in paraffin and sectioned serially in transverse direction. BMP-2 and BMP-4 protein expression in the developing neural tube and the caudal spinal cord was determined by immunohistochemistry. Our data show that BMP-s tend to be more expressed in the neural tube in earlier stages; in particular, BMP-4 staining was found to be higher at CS10 compared to CS20. More detailed analysis was performed on embryos of CS14-18. Stronger BMP-2 and BMP-4 expression was found in the dorsal part than in the ventral part of the spinal cord. No differences were seen in the staining intensity of BMP-s in the spinal ganglia. Interestingly, in neural crest cells BMP-2 staining was stronger at CS16 and CS18 as compared to CS14, while no differences were found in BMP-4 staining. On the other hand, in the non-neural ectoderm BMP-4 staining was found to be stronger at CS16 than at CS14, while no differences were seen for BMP-2. In conclusion, expression of BMP-s in the developing neural tube and spinal cord of human embryos is generally in accordance with the findings made in rodents and birds.
author	Namm,Aimar Arend,Andres Aunapuu,Marina
author_facet	Namm,Aimar Arend,Andres Aunapuu,Marina
author_sort	Namm,Aimar
title	Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human Embryos
title_short	Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human Embryos
title_full	Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human Embryos
title_fullStr	Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human Embryos
title_full_unstemmed	Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human Embryos
title_sort	immunohistochemical detection of bmp-2 and bmp-4 in the developing neural tube and spinal cord of human embryos
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200018
work_keys_str_mv	AT nammaimar immunohistochemicaldetectionofbmp2andbmp4inthedevelopingneuraltubeandspinalcordofhumanembryos AT arendandres immunohistochemicaldetectionofbmp2andbmp4inthedevelopingneuraltubeandspinalcordofhumanembryos AT aunapuumarina immunohistochemicaldetectionofbmp2andbmp4inthedevelopingneuraltubeandspinalcordofhumanembryos
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Immunohistochemical Detection of BMP-2 and BMP-4 in the Developing Neural Tube and Spinal Cord of Human Embryos

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